公开(公告)号：US5962292A

公开(公告)日：1999-10-05

申请号：US708856

申请日：1996-09-09

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

IPC分类号： C12N9/02 ,  C12P13/24 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.

摘要翻译： 用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。 一种新的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用转化体产生L-脯氨酸-3-羟化酶的方法。

公开(公告)号：US07112432B2

公开(公告)日：2006-09-26

申请号：US10864423

申请日：2004-06-10

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

IPC分类号： C12N1/20 ,  C12N15/75 ,  C12N15/76 ,  C07H21/04

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/832 ,  Y10S435/886

摘要： An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

公开(公告)号：US06767726B2

公开(公告)日：2004-07-27

申请号：US10138632

申请日：2002-05-06

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

IPC分类号： C12N902

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

摘要： An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

摘要翻译： 分离的基因编码具有SEQ ID NO：1,2,15,16和17的氨基酸序列的L-脯氨酸-3-羟化酶，或编码具有酶活性的蛋白质，以使L-脯氨酸的3-位羟基化 并在2-酮戊二酸和二价铁离子存在下作用于游离的L-脯氨酸以产生顺式-3-羟基-L-脯氨酸。 将该基因插入载体中，并将​​所得的重组DNA用于产生转化体。 通过在培养基中培养转化体产生L-脯氨酸-3-羟化酶。

公开(公告)号：US6010891A

公开(公告)日：2000-01-04

申请号：US287375

申请日：1999-04-07

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

IPC分类号： C12N9/02 ,  C12P13/24 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant, is also provided.

摘要翻译： 用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。 一种新型的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用该转化体产生L-脯氨酸-3-羟化酶的方法， 也提供。

公开(公告)号：US06413748B1

公开(公告)日：2002-07-02

申请号：US09455406

申请日：1999-12-06

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

IPC分类号： C12P1324

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.

摘要翻译： 用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。 一种新的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用转化体产生L-脯氨酸-3-羟化酶的方法。

公开(公告)号：US5854040A

公开(公告)日：1998-12-29

申请号：US709874

申请日：1996-09-09

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

IPC分类号： C12N9/02 ,  C12P13/24

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

摘要： Isolated and purified L-proline-4-hydroxylase which catalyzes hydroxylation of L-proline at the 4-position of L-proline in the presence of 2-ketoglutaric acid and a divalent iron ion to produce trans-4-hydroxy-L-proline. The L-proline-4-hydroxylase may be isolated from a microorganism selected from the group consisting of Dactylosporanlium sp. RHI deposited with the National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology, Japan, under the designation PERM BP-4400 and Amycolatopsis sp. RH2 deposited as FERM BP-4581.

摘要翻译： 分离和纯化的L-脯氨酸-4-羟化酶，其在2-酮戊二酸和二价铁离子的存在下催化L-脯氨酸的4-位的L-脯氨酸的羟化作用，以产生反式-4-羟基-L-脯氨酸 。 L-脯氨酸-4-羟化酶可以从选自Dactylosporanium sp。 RHI保藏于日本工业科学与技术国家生物科学与人类技术研究所，名称为PERM BP-4400和Amycolatopsis sp。 RH2沉积为FERM BP-4581。

公开(公告)号：US20090263885A1

公开(公告)日：2009-10-22

申请号：US11797687

申请日：2007-05-07

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

IPC分类号： C12N1/21

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

摘要： The present invention is directed to a process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. In addition, the present invention is directed to a process for producing trans-4-hydroxy-L-proline, wherein the L-proline biosynthesis activity of the host cell of the transformant is reinforced.

摘要翻译： 本发明涉及一种生产反式-4-羟基-L-脯氨酸的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于Dactylosporangium，Amycolatopsis或Streptomyces属的微生物的酶源的存在下转化成反式-4-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化 在水性介质中转化成反式-4-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，从水性介质中收集生成的反式-4-羟基-L-脯氨酸。 此外，本发明涉及一种生产反式-4-羟基-L-脯氨酸的方法，其中转化体宿主细胞的L-脯氨酸生物合成活性得到增强。

公开(公告)号：US06242231B1

公开(公告)日：2001-06-05

申请号：US09104382

申请日：1998-06-25

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

IPC分类号： C12N902

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

摘要： An industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity.

摘要翻译： 用于生产反式-4-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品的添加剂。 在此过程中，L-脯氨酸在衍生自属于Dactylosporangium，Amycolatopsis或Streptomyces属的微生物的酶源的存在下转化成反式-4-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化 在水性介质中转化成反式-4-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，从水性介质中收集生成的反式-4-羟基-L-脯氨酸。 还提供了可用于该方法的新型L-脯氨酸-4-羟化酶，可用于该方法的L-脯氨酸-4-羟化酶的基因，含有该基因的转化体的基因， 脯氨酸-4-羟化酶。 此外，提供了使用含有该基因并具有增强的脯氨酸生物合成活性的转化体生产L-脯氨酸-4-羟化酶的方法。

公开(公告)号：US07238501B2

公开(公告)日：2007-07-03

申请号：US10610561

申请日：2003-07-02

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

IPC分类号： C12P13/24

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

摘要： The present invention is directed to a process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. In addition, the present invention is directed to a process for producing trans-4-hydroxy-L-proline, wherein the L-proline biosynthesis activity of the host cell of the transformant is reinforced.

摘要翻译： 本发明涉及一种生产反式-4-羟基-L-脯氨酸的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于Dactylosporangium，Amycolatopsis或Streptomyces属的微生物的酶源的存在下转化成反式-4-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化 在水性介质中转化成反式-4-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，从水性介质中收集生成的反式-4-羟基-L-脯氨酸。 此外，本发明涉及一种生产反式-4-羟基-L-脯氨酸的方法，其中转化体宿主细胞的L-脯氨酸生物合成活性得到增强。

公开(公告)号：US06617140B2

公开(公告)日：2003-09-09

申请号：US09833555

申请日：2001-04-13

申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

IPC分类号： C12P1324

CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

摘要： Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity.