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公开(公告)号：US08058410B2
公开(公告)日：2011-11-15
申请号：US11919258
申请日：2006-04-25
申请人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmüller , Philipp Wechner
发明人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmüller , Philipp Wechner
摘要： Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula X1X2X3X4, wherein X1 to X4 are amino acid residues and at least two of X1 to X4 is W, Y or F; b) peptides comprising the formula X5X6X7X8, wherein X5 to X8 are amino acid residues, at least one of X5 to X8 is W, and at least one of X5 to X8 is E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.
摘要翻译： 公开了包含固相和包含与该固相偶联的肽键的亲和配体的亲和基质，其中包含肽键的亲和配体选自以下配体组：a）包含式X1X2X3X4的肽，其中X1至X4 是氨基酸残基，X1至X4中的至少两个为W，Y或F; b）包含式X5X6X7X8的肽，其中X5至X8为氨基酸残基，X5至X8中的至少一个为W，X5至X8中的至少一个为E或D; 和c）由R，K，E和D组成的组的氨基酸单体和由Y，F和W组成的组的氨基酸单体组成的聚氨基酸，优选聚KY，聚-KF， poly-KW，poly-RY，poly-RF，poly-RW，poly-EY，poly-DY，poly-EF，poly-EW，poly-DF和poly-DW，条件是根据a） 和b）具有35个氨基酸残基的最大长度，并且根据c）的多氨基酸具有20个氨基酸残基的最小长度。
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公开(公告)号：US20090306343A1
公开(公告)日：2009-12-10
申请号：US11919258
申请日：2006-04-25
申请人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmuller , Philipp Wechner
发明人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmuller , Philipp Wechner
摘要： Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula X1X2X3X4, wherein X1 to X4 are amino acid residues and at least two of X1 to X4 is W, Y or F; b) peptides comprising the formula X5X6X7X8, wherein X5 to X8 are amino acid residues, at least one of X5 to X8 is W, and at least one of X5 to X8 is E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.
摘要翻译： 公开了包含固相和包含与该固相偶联的肽键的亲和配体的亲和基质，其中包含肽键的亲和配体选自以下配体组：a）包含式X1X2X3X4的肽，其中X1至X4 是氨基酸残基，X1至X4中的至少两个为W，Y或F; b）包含式X5X6X7X8的肽，其中X5至X8为氨基酸残基，X5至X8中的至少一个为W，X5至X8中的至少一个为E或D; 和c）由R，K，E和D组成的组的氨基酸单体和由Y，F和W组成的组的氨基酸单体组成的聚氨基酸，优选聚KY，聚-KF， poly-KW，poly-RY，poly-RF，poly-RW，poly-EY，poly-DY，poly-EF，poly-EW，poly-DF和poly-DW，条件是根据a） 和b）具有35个氨基酸残基的最大长度，并且根据c）的多氨基酸具有20个氨基酸残基的最小长度。
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3.发明授权Production of recombinant proteins by autoproteolytic cleavage of a fusion protein 有权通过自身蛋白水解切割融合蛋白来生产重组蛋白公开(公告)号：US08163890B2
公开(公告)日：2012-04-24
申请号：US11919257
申请日：2006-04-25
摘要： Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
摘要翻译： 公开了使用包含感兴趣的多肽的融合多肽和N末端具有表达自身蛋白水解功能的多肽来产生具有均质N末端的异源多肽的方法，所述方法包括以下步骤：a） 通过亲和层析系统以可溶性，自动蛋白水解无活性形式的融合多肽，b）融合多肽的折叠，从而激活融合多肽的自体蛋白水解功能，并导致感兴趣的异源多肽切割，以及c）随后将异源 多肽，其中所述步骤在一个亲和层析系统上进行。
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公开(公告)号：US20090203069A1
公开(公告)日：2009-08-13
申请号：US11919257
申请日：2006-04-25
摘要： Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
摘要翻译： 公开了使用包含感兴趣的多肽的融合多肽和N末端具有表达自身蛋白水解功能的多肽来产生具有均质N末端的异源多肽的方法，所述方法包括以下步骤：a） 通过亲和层析系统以可溶性，自动蛋白水解无活性形式的融合多肽，b）融合多肽的折叠，从而激活融合多肽的自体蛋白水解功能，并导致感兴趣的异源多肽切割，以及c）随后将异源 多肽，其中所述步骤在一个亲和层析系统上进行。
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5.发明授权Production of recombinant proteins by autoproteolytic cleavage of a fusion protein 有权通过自身蛋白水解切割融合蛋白来生产重组蛋白公开(公告)号：US08372959B2
公开(公告)日：2013-02-12
申请号：US13348607
申请日：2012-01-11
摘要： Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprises the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein the steps are conducted on one affinity chromatography system.
摘要翻译： 公开了使用包含感兴趣的多肽的融合多肽和N末端具有表达自身蛋白水解功能的多肽来产生具有均质N末端的异源多肽的方法，所述方法包括以下步骤：a） 通过亲和层析系统以可溶性，自动蛋白水解无活性形式的融合多肽，b）融合多肽的折叠，从而激活融合多肽的自体蛋白水解功能，并导致感兴趣的异源多肽切割，以及c）随后将异源 多肽，其中所述步骤在一个亲和层析系统上进行。
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公开(公告)号：US06664305B2
公开(公告)日：2003-12-16
申请号：US10182768
申请日：2002-10-28
申请人： Alois Jungbauer , Rainer Hahn , Ales Podgornik , Milos Barut , Ales Strancar
发明人： Alois Jungbauer , Rainer Hahn , Ales Podgornik , Milos Barut , Ales Strancar
IPC分类号： C08J928
CPC分类号： G01N30/482 , B01D15/361 , B01D15/3804 , B01D15/3809 , B01J20/26 , B01J20/28042 , B01J20/285 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/327 , B01J20/3272 , B01J20/3282 , B01J2220/54 , B01J2220/82
摘要： A process of manufacturing of a chromatography material comprising the steps of (i) reacting a polymerizable at least bifunctional monomer A with a ligand also having a functional group which binds covalently with one of the functional groups of said polymerizable bifunctional monomer A, (ii) to form a compound B comprising at least one polymerizable functional moiety (iii) polymerizing the compound B essentially alone or with the polymerizable monomers in presence of porogenes to obtain a block of porous polymerizate which is self-supporting or (iv) reacting the ligand and a spacer via a covalent bond which is cleavable to form a ligand-spacer conjugate and binding the ligand-spacer conjugate to the surface of a chromatographic support or reacting the ligand-spacer conjugate via a covalent bond to the at least bi-functional monomer A and polymerizing it essentially alone or with the polymerizable monomers in presence of porogenes to obtain a block of porous polymerizate which is self-supporting.
摘要翻译： 一种制备色谱材料的方法，包括以下步骤：（i）使可聚合的至少双官能单体A与还具有与所述可聚合双官能单体A的一个官能团共价键合的官能团的配体反应，（ii） 以形成包含至少一个可聚合官能部分的化合物B（iii）在致孔剂存在下基本上单独或与可聚合单体聚合化合物B以获得自支撑的多孔聚合物嵌段，或（iv）使配体和 通过共价键的间隔物，其可切割以形成配体 - 间隔基缀合物并将配体 - 间隔物缀合物结合到色谱载体的表面或通过共价键与配体 - 间隔物缀合物反应至至少双官能单体A 并在致孔剂存在下基本上单独或与可聚合单体聚合，得到多孔聚合物嵌段，其为自支撑 锻炼。
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公开(公告)号：US20070243201A1
公开(公告)日：2007-10-18
申请号：US10559131
申请日：2004-06-02
申请人： Hans Loibner , Gottfried Himmler , Alois Jungbauer , Erich Wasserbauer , Manfred Schuster , Rainer Hahn , Astrid Durauer
发明人： Hans Loibner , Gottfried Himmler , Alois Jungbauer , Erich Wasserbauer , Manfred Schuster , Rainer Hahn , Astrid Durauer
CPC分类号： C07K16/3076 , A61K2039/505 , C07K14/4748
摘要： The invention relates to a method for the selection of epitopes for immunotherapy, peptides obtained by said method, the use of said peptides as vaccines and diagnostics and an immune serum obtainable by said method.
摘要翻译： 本发明涉及用于选择免疫治疗表位的方法，通过所述方法获得的肽，所述肽作为疫苗和诊断的用途以及通过所述方法可获得的免疫血清。
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公开(公告)号：US07060460B2
公开(公告)日：2006-06-13
申请号：US10261508
申请日：2002-10-02
IPC分类号： C12P21/06
CPC分类号： C07K1/1136 , C07K1/16
摘要： In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.
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9.发明授权Method for sterilizing liquid chromatography resins highly resistant to oxidation and a sterilization solution for use therein 失效用于对高度耐氧化的液相色谱树脂灭菌的方法和用于其中的灭菌溶液
公开(公告)号：US5676837A
公开(公告)日：1997-10-14
申请号：US662262
申请日：1996-06-07
IPC分类号： G01N30/50 , A01N37/16 , A61L2/18 , B01D15/08 , B01J20/281
CPC分类号： B01J20/281 , A01N37/16 , A61L2/18 , G01N30/48 , B01J2220/54 , G01N30/50
摘要： A method for sterilizing liquid chromatography resins that are highly resistant to oxidation by strongly oxidizing agents and a sterilization solution for use therein. In a preferred embodiment, the invention comprises contacting an oxidant-resistant chromatographic resin with an aqueous solution of peracetic acid and up to 40% ethanol. In particular, where the solution is to be used to sterilize a positively charged ion-exchange resin, the solution preferably additionally includes an acetate buffer of a sufficient ionic strength to prevent adsorption of the peracetic acid ions on the resin.
摘要翻译： 一种对强氧化剂高度耐氧化的液相色谱树脂和用于其中的杀菌溶液进行灭菌的方法。 在优选的实施方案中，本发明包括使抗氧化剂的色谱树脂与过乙酸的水溶液和高达40％的乙醇接触。 特别是在将溶液用于对带正电荷的离子交换树脂进行灭菌的情况下，溶液优选另外包括具有足够的离子强度的乙酸盐缓冲液，以防止过乙酸离子吸附在树脂上。
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公开(公告)号：US20090182028A1
公开(公告)日：2009-07-16
申请号：US12295285
申请日：2007-03-29
IPC分类号： A61K31/404 , C07D405/06 , A61P35/00 , A61P25/28
CPC分类号： A61K31/404 , A23L33/105 , A23V2002/00 , A61K31/352 , A61K31/353 , A61K31/366 , A61K36/31 , A23V2250/21 , A23V2200/308
摘要： The present invention provides a medicament comprising a chemical compound according to formula A: wherein R1 is hydrogen, fluorine, chlorine, bromine, hydroxy, mercapto, methoxy, ethoxy, acetoxy, methyl, ethyl, propyl, isopropyl, t-butyl, nitro, amin, N,N-dimethylaminoyl, N,N-diethyl-aminoyl; R2 is hydrogen, a C1-8-alkyl e.g. methyl, ethyl, propyl, isopropyl, butyl, t-butyl; an C1-8-acyl e.g. acetyl, propionyl; R3 is a C5-12-heterocyclic ring, e.g. furanyl, pyranyl, pyrrolyl, pyridinyl, pyrazolyl, thienyl, thiazolyl, indolyl or -0R4 ; R4 is a C5-C12-aromatic ring e.g. phenyl; unsubstituted or substituted in any position with fluorine, chlorine, bromine, methoxy, ethoxy, C1-8-carboxy; Z is OH or 0; and n is 0, 1, 2 or 3, and m is 0, 1, 2 or 3, and its use for the activation of the aryl hydro carbon receptor.
摘要翻译： 本发明提供一种药物，其包含式A化合物：其中R 1是氢，氟，氯，溴，羟基，巯基，甲氧基，乙氧基，乙酰氧基，甲基，乙基，丙基，异丙基，叔丁基，硝基， 氨基，N，N-二甲基氨基，N，N-二乙基 - 氨基; R 2是氢，C 1-8 - 烷基， 甲基，乙基，丙基，异丙基，丁基，叔丁基; 例如C 1-8酰基。 乙酰基，丙酰基; R3是C5-12-杂环，例如 呋喃基，吡喃基，吡咯基，吡啶基，吡唑基，噻吩基，噻唑基，吲哚基或-OR4; R4是C5-C12芳族环。 苯基; 在任何位置用氟，氯，溴，甲氧基，乙氧基，C1-8-羧基取代或取代; Z是OH或0; 并且n为0,1,2或3，m为0,1,2或3，并且其用于活化芳基氢碳受体。
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