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    Biologically active metal-coated proteins
    5.
    发明申请
    Biologically active metal-coated proteins 审中-公开
    生物活性金属包被的蛋白质

    公开(公告)号：US20090127112A1

    公开(公告)日：2009-05-21

    申请号：US11920689

    申请日：2006-05-18

    申请人： Amihay Freeman ,  Yosi Shacham-Diamand ,  Sefi Vernick ,  Hila Moscovich-Dagan

    发明人： Amihay Freeman ,  Yosi Shacham-Diamand ,  Sefi Vernick ,  Hila Moscovich-Dagan

    IPC分类号： G01N33/487 ,  C12N9/04 ,  A61K9/00 ,  A61K38/44

    CPC分类号： G01N33/54373 ,  A61B5/1486 ,  G01N33/532 ,  G01N33/68 ,  G01N33/84 ,  Y10T428/12493

    摘要： Metal-coated proteins, being dissolvable or suspendable in aqueous media and/or retaining a biological activity of the protein, a process and intermediates for preparing same are disclosed. Further disclosed are a pharmaceutical composition containing and a method of treating bacterial and fungal infections utilizing biologically active metal-coated proteins. Conductive elements, electronic circuits containing same, electrodes and biosensor systems utilizing same, and imaging probes, all containing the metal-coated proteins, are also disclosed.

    摘要翻译： 公开了金属包覆的蛋白质，其可溶于或悬浮于水性介质中和/或保留蛋白质的生物活性，制备方法和中间体。 还公开了一种药物组合物，其含有利用生物活性金属包被的蛋白质治疗细菌和真菌感染的方法。 还公开了导电元件，含有它们的电子电路，使用它们的电极和生物传感器系统，以及全部含有金属包被的蛋白质的成像探针。

    Thromboresistant surface treatment for biomaterials
    6.
    发明授权
    Thromboresistant surface treatment for biomaterials 失效
    生物材料的抗血栓表面处理

    公开(公告)号：US5578073A

    公开(公告)日：1996-11-26

    申请号：US37335

    申请日：1994-09-16

    申请人： Beatrice Haimovich ,  Amihay Freeman ,  Ralph Greco

    发明人： Beatrice Haimovich ,  Amihay Freeman ,  Ralph Greco

    IPC分类号： A61F2/06 ,  A61L33/06 ,  C09D105/08 ,  A61F2/16 ,  C08L5/08

    CPC分类号： C09D105/08 ,  A61F2/06 ,  A61L33/062

    摘要： A biomaterial with a thromboresistant surface and a method for forming same are provided. The thromboresistant surface is comprised of a distinct coating layer of a chitosan-based membrane and a biologically active material. The biologically active material is capable of converting the chitosan membrane coating from a highly thrombogenic to an essentially non-thrombogenic one. The biologically active material can be a polymeric substance, such as polyvinyl alcohol, forming a polymeric blend with the chitosan, or, can be a biological substance, such as serum albumin, embedded in or attached to the chitosan membrane which has been activated with a treatment of glutardialdehyde. The thromboresistant biomaterial is suitable for use in vascular grafts having an inside diameter of less than 6 millimeters.

    摘要翻译： 提供具有抗血栓表面的生物材料及其形成方法。 抗血栓表面由基于壳聚糖的膜和生物活性物质的不同的涂层组成。 生物活性物质能够将壳聚糖膜涂层从高度血栓形成转变为基本上非致血性的。 生物活性材料可以是聚合物质，例如聚乙烯醇，与壳聚糖形成聚合物共混物，或者可以是嵌入或连接到壳聚糖膜上的生物物质，例如血清白蛋白，其已经用 治疗戊二醛。 抗血栓生物材料适用于内径小于6毫米的血管移植物。

    Stabilized enzymes
    7.
    发明授权
    Stabilized enzymes 失效
    稳定酶

    公开(公告)号：US4904592A

    公开(公告)日：1990-02-27

    申请号：US219927

    申请日：1988-07-15

    申请人： Amihay Freeman ,  Ruth Tor

    发明人： Amihay Freeman ,  Ruth Tor

    IPC分类号： C12N9/96 ,  C12N11/08

    CPC分类号： C12N9/96 ,  C12N11/08

    摘要： Enzymes are stabilized by encagement in a double layer having a polyaldeh base coat linked to the amino groups of the enzyme, and an outer polymer coat crosslinked therewith. The outer coat is made of a polymer which in the unlinked state has free amino and/or acyl hydrazide groups.Optionally the stabilized enzyme is immobilized within a matrix.Processes for the preparation of the encaged and optionally immobilized enzymes are described.

    Controlled enzymatic removal and retrieval of cells
    9.
    发明授权
    Controlled enzymatic removal and retrieval of cells 有权
    控制细胞的酶促去除和回收

    公开(公告)号：US07364565B2

    公开(公告)日：2008-04-29

    申请号：US10768749

    申请日：2004-01-27

    申请人： Amihay Freeman

    发明人： Amihay Freeman

    IPC分类号： A61N1/30

    CPC分类号： A61M3/0258 ,  A61B10/0035 ,  A61B10/02 ,  A61B17/545 ,  A61B2017/00761 ,  A61B2017/00769 ,  A61B2017/00774 ,  A61B2017/22082 ,  A61B2017/3411 ,  A61K38/48 ,  A61K38/4886 ,  A61M1/0088 ,  A61M3/0241 ,  A61M3/0283 ,  A61M3/0287 ,  A61M27/00 ,  C12M45/09 ,  C12N5/0629 ,  C12N2509/00

    摘要： Method and device, including a uniquely operative applicator, and pharmaceutical compositions, for the controlled, non-surgical removal and retrieval of cells from a variety of skin lesions and tissue surfaces are disclosed. A synergistic effect of proteolytic digestion of the intracellular matrix and “stripping” flow is achieved by treating a defined area with a controlled, continuous stream of proteolytic enzyme solution, causing gentle but effective tissue erosion. Isolated cells from the skin lesion and/or tissue surface may be collected from the protease solution stream for histological analysis and/or cell culture, affording a method of “enzymatic biopsy”. The protease solution may be supplemented with anesthetics, coagulants, anticoagulants and antibiotics to decrease the discomfort, erythema, bleeding, risk of infection and scarring traditionally associated with surgical treatment of skin lesions. Delivery of precise levels of catalytic activity is ensured by controlled activation of stable, inactivated enzyme stock solutions and powders shortly prior to application.

    摘要翻译： 公开了包括独特操作的施用器和药物组合物的方法和装置，用于从各种皮肤损伤和组织表面控制，非手术移除和取回细胞。 通过用受控的连续的蛋白水解酶溶液处理限定区域来实现细胞内基质的蛋白水解消化和“剥离”流的协同效应，引起温和但有效的组织侵蚀。 可以从蛋白酶溶液流中收集来自皮肤病变和/或组织表面的分离的细胞用于组织学分析和/或细胞培养，提供“酶活检”的方法。 蛋白酶溶液可以用麻醉剂，凝结剂，抗凝剂和抗生素补充，以减少传统上与皮肤损伤手术治疗相关的不适，红斑，出血，感染风险和瘢痕。 通过在施用前不久稳定的灭活酶储备溶液和粉末的控制活化来确保提供精确水平的催化活性。

    Dihaloisocyanide derivatives of polymers for coupling nucleophiles
    10.
    发明授权
    Dihaloisocyanide derivatives of polymers for coupling nucleophiles 失效
    用于偶联亲核体的聚合物的二卤代吖啶衍生物

    公开(公告)号：US4440903A

    公开(公告)日：1984-04-03

    申请号：US921055

    申请日：1978-06-30

    申请人： Leon Golstein ,  Mordechai Sokolovsky ,  Amihay Freeman

    发明人： Leon Golstein ,  Mordechai Sokolovsky ,  Amihay Freeman

    IPC分类号： C08G12/40 ,  C08G63/685 ,  C08G63/91 ,  C08G85/00 ,  C12N11/08 ,  C07C143/00 ,  C07G7/00 ,  C12N11/06

    CPC分类号： C08G63/91 ,  C08G12/40 ,  C08G63/685 ,  C08G85/004 ,  C12N11/08 ,  Y10S525/921

    摘要： The present invention relates to novel key intermediates for the production of biologically active compounds coupled to polymers, of the general formula P--NCX.sub.2, wherein X designates a halogen atom selected from chlorine and bromine, and wherein P designates the polymer backbone of a polymer of the polyamide, polyester and ureaformaldehyde type. The invention further relates to compounds obtained by the reaction of the above compound P--NCX.sub.2 with a bifunctional or polyfunctional nucleophile. Suitable nucleophiles are hydrazides of dicarboxylic acids and amongst these there may be mentioned polyacrylamides partially substituted with acylhydrazide groups; polyfunctional amines selected from alkylamines, aralkylamines, arylamines and macromolecular compounds containing amino groups. The key intermediate PNCX.sub.2 may be coupled directly to biologically active macromolecules and amongst the preferred compounds of this type there are the various biologically active proteins and enzymes. The coupling of the biologically active macromolecules may also be effected via hydrophilic polymer moieties first grafted onto the polymer P via said dihaloisocyanide groups.

    摘要翻译： 本发明涉及用于生产与通式为P-NCX2的聚合物偶联的生物活性化合物的新型关键中间体，其中X表示选自氯和溴的卤素原子，其中P表示聚合物的聚合物主链 聚酰胺，聚酯和脲甲醛型。 本发明还涉及通过上述化合物P-NCX2与双官能或多官能亲核试剂的反应获得的化合物。 合适的亲核试剂是二羧酸的酰肼，其中可以提及部分被酰基酰肼基团取代的聚丙烯酰胺; 选自烷基胺，芳烷基胺，芳基胺和含有氨基的高分子化合物的多官能胺。 关键的中间体PNCX2可以直接与生物活性大分子偶联，并且在这种类型的优选化合物中存在各种生物活性蛋白质和酶。 生物活性大分子的偶联也可以通过首先通过所述二卤代吖啶基接枝到聚合物P上的亲水性聚合物部分来实现。