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公开(公告)号：US20090075287A1
公开(公告)日：2009-03-19
申请号：US12208629
申请日：2008-09-11
申请人： Andre R. Abad , Ronald D. Flannagan , Bin Hu , Billy F. McCutchen , Xiaofeng Sean Yang , Cao Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Bin Hu , Billy F. McCutchen , Xiaofeng Sean Yang , Cao Guo Yu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/689 , C12Q2541/101
摘要： Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.
摘要翻译： 提供了鉴定新型杀虫基因同系物的方法和组合物。 具体地说，本发明的方法包括系统地设计针对目的杀虫基因特异性的寡核苷酸引物，并对来自微生物特别是苏云金芽孢杆菌属菌株的核酸材料进行连续循环的PCR扩增，以鉴定已知杀虫基因的新型同系物 。 进一步公开了可用于实施本方法的寡核苷酸引物。
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公开(公告)号：US07452700B2
公开(公告)日：2008-11-18
申请号：US11404741
申请日：2006-04-14
申请人： Andre R. Abad , Ronald D. Flannagan , Bin Hu , Billy F. McCutchen , Xiaofeng Sean Yang , Cao Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Bin Hu , Billy F. McCutchen , Xiaofeng Sean Yang , Cao Guo Yu
CPC分类号： C12Q1/6809 , C12Q1/689 , C12Q2541/101
摘要： Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.
摘要翻译： 提供了鉴定新型杀虫基因同系物的方法和组合物。 具体地说，本发明的方法包括系统地设计针对目的杀虫基因特异性的寡核苷酸引物，并对来自微生物特别是苏云金芽孢杆菌属菌株的核酸材料进行连续循环的PCR扩增，以鉴定已知杀虫基因的新型同系物 。 进一步公开了可用于实施本发明方法的寡核苷酸引物。
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公开(公告)号：US08071736B2
公开(公告)日：2011-12-06
申请号：US12512425
申请日：2009-07-30
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US07491536B2
公开(公告)日：2009-02-17
申请号：US11692967
申请日：2007-03-29
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
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公开(公告)号：US20070245430A1
公开(公告)日：2007-10-18
申请号：US11692973
申请日：2007-03-29
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了新型多核苷酸及其编码的杀虫多肽。 还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。 本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。
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公开(公告)号：US20070243614A1
公开(公告)日：2007-10-18
申请号：US11692967
申请日：2007-03-29
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
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公开(公告)号：US07629449B2
公开(公告)日：2009-12-08
申请号：US11949912
申请日：2007-12-04
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： In particular aspects of the invention, nucleic acid molecules encoding novel proteases identified in the midgut of Diabrotica virgfera virgfera (i.e., western corn rootworm) are provided. The nucleotide sequences encode novel cysteine proteases that belong to the cathepsin L-like subfamily of proteases. Variants and fragments of the nucleotide sequences that encode polypeptide sequences that possess proteolytic activity are further disclosed. Methods of using the nucleic acid sequences that encode novel proteases to protect a plant from an insect pest are provided. Particular embodiments also provide vectors, expression cassettes and transformed host cells comprising a protease nucleic acid of the invention.
摘要翻译： 在本发明的特定方面，提供了编码在维生素Diabrotica virgfera（即西方玉米根虫）的中肠中鉴定的新型蛋白酶的核酸分子。 核苷酸序列编码属于蛋白酶组织蛋白酶L样亚类的新型半胱氨酸蛋白酶。 还公开了编码具有蛋白水解活性的多肽序列的核苷酸序列的变体和片段。 提供了使用编码新型蛋白酶的核酸序列来保护植物免受害虫的方法。 具体实施方案还提供载体，表达盒和包含本发明的蛋白酶核酸的转化宿主细胞。
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公开(公告)号：US07329736B2
公开(公告)日：2008-02-12
申请号：US11404638
申请日：2006-04-14
IPC分类号： C07K14/415 , C07H21/04
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了新型多核苷酸及其编码的杀虫多肽。 还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。 本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。
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公开(公告)号：US07910807B2
公开(公告)日：2011-03-22
申请号：US11940002
申请日：2007-11-14
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US07803993B2
公开(公告)日：2010-09-28
申请号：US11853849
申请日：2007-09-12
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了新型多核苷酸及其编码的杀虫多肽。 还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。 本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。
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