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    Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses
    7.
    发明申请
    Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses 审中-公开
    提高细胞电穿孔效率的方法

    公开(公告)号：US20090000948A1

    公开(公告)日：2009-01-01

    申请号：US12097409

    申请日：2007-01-04

    申请人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

    发明人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

    IPC分类号： G01N27/26 ,  G01N27/00

    CPC分类号： C12N13/00 ,  C12M23/16 ,  C12M33/00 ,  C12M35/02

    摘要： The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.

    摘要翻译： 本发明提供使用介电电泳辅助细胞定位提高细胞电穿孔效率的方法及其在微流体生物芯片系统中的用途。 细胞首先进行介导电泳，并定位于电场强度足以使细胞电穿孔的区域。 本发明增强了传统微流体生物芯片上原位细胞电穿孔的效率。

    Method of operating a tubular string assembly within a wellbore
    8.
    发明授权
    Method of operating a tubular string assembly within a wellbore 有权

    公开(公告)号：US11365608B2

    公开(公告)日：2022-06-21

    申请号：US16148684

    申请日：2018-10-01

    申请人： Haining Zheng ,  Lei Wang ,  Michael W. Walker

    发明人： Haining Zheng ,  Lei Wang ,  Michael W. Walker

    IPC分类号： E21B41/00 ,  E21B47/00 ,  E21B44/00 ,  E21B21/08 ,  E21B21/06

    摘要： A method of moving a string assembly within a wellbore is disclosed. In some embodiments, the method comprises moving the string assembly within the wellbore; obtaining surface data regarding at least one parameter associated with moving the string assembly within the wellbore over a range of depths; modeling the at least one parameter over the range of depths for a plurality of assumed friction factors to obtain modeled data for each assumed friction factor; calculating a derivative of the surface data over the range of depths; calculating a derivative of the modeled data over the range of depths; comparing the derivative of the surface data to the derivative of the modeled data; determining one or more local friction factors for the range of depths based on the comparison; and adjusting at least one string assembly operating parameter based on the one or more local friction factors.

    Method of Operating a Tubular String Assembly within a Wellbore
    10.
    发明申请
    Method of Operating a Tubular String Assembly within a Wellbore 审中-公开

    公开(公告)号：US20190178059A1

    公开(公告)日：2019-06-13

    申请号：US16148684

    申请日：2018-10-01

    申请人： Haining Zheng ,  Lei Wang ,  Michael W. Walker

    发明人： Haining Zheng ,  Lei Wang ,  Michael W. Walker

    IPC分类号： E21B41/00 ,  E21B47/00

    摘要： A method of moving a string assembly within a wellbore is disclosed. In some embodiments, the method comprises moving the string assembly within the wellbore; obtaining surface data regarding at least one parameter associated with moving the string assembly within the wellbore over a range of depths; modeling the at least one parameter over the range of depths for a plurality of assumed friction factors to obtain modeled data for each assumed friction factor; calculating a derivative of the surface data over the range of depths; calculating a derivative of the modeled data over the range of depths; comparing the derivative of the surface data to the derivative of the modeled data; determining one or more local friction factors for the range of depths based on the comparison; and adjusting at least one string assembly operating parameter based on the one or more local friction factors.