利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

20 条记录 (耗时 0.021 秒)

    DNA sequencing with pyrophosphatase
    2.
    发明授权
    DNA sequencing with pyrophosphatase 失效
    利用焦磷酸酶进行DNA测序

    公开(公告)号：US5498523A

    公开(公告)日：1996-03-12

    申请号：US275339

    申请日：1994-07-14

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12N9/16 ,  C12Q1/68 ,  C12N9/14 ,  C12P19/34 ,  G01N33/50

    CPC分类号： C12N9/16 ,  C12Q1/686 ,  Y10T436/143333

    摘要： A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

    摘要翻译： 一种用于延伸寡核苷酸引物的试剂盒或溶液，所述寡核苷酸引物在具有与第一单链区同源的第二单链区的模板分子上具有第一单链区，其包含能够引起第一 在反应混合物中在模板的第二单链区上的引物的单链区域，以及能够将反应混合物中的焦磷酸盐的量降低到在不存在第二次的情况下在扩展期间产生的量的第二试剂 代理商

    DNA sequencing
    5.
    发明授权
    DNA sequencing 失效
    DNA测序

    公开(公告)号：US5674716A

    公开(公告)日：1997-10-07

    申请号：US422147

    申请日：1995-04-13

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12Q1/42 ,  C12Q1/48 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/70 ,  C07K3/14

    CPC分类号： C12Q1/6869 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/808 ,  Y10T436/143333

    摘要： A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

    摘要翻译： 一种测序DNA链的方法，包括以下步骤：提供DNA链; 用能够与链杂交的引物退火链，得到退火混合物; 在聚合酶引导引物伸长的条件下，使DNA混合物与脱氧核糖核苷三磷酸，DNA聚合酶和链终止剂一起孵育以形成长链引物长度不同的一系列DNA产物，每个DNA产物具有链 终止剂在其细长端; 对于从1至20个碱基长度不同的基本上所有DNA产物，每个DNA产物的数量大致相同。

    Method for sequencing DNA using a T7-type DNA polymerase and short oligonucleotide primers
    6.
    发明授权
    Method for sequencing DNA using a T7-type DNA polymerase and short oligonucleotide primers 失效
    使用T7型DNA聚合酶和短寡核苷酸引物对DNA进行测序的方法

    公开(公告)号：US5639608A

    公开(公告)日：1997-06-17

    申请号：US196538

    申请日：1994-02-14

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6869

    摘要： This invention relates to methods for determining the nucleotide base sequence of a deoxyribose nucleic acid molecule comprising the steps of incubating the nucleic acid molecule with an oligonucleotide primer of 5 to 8 bases in length, a plurality of deoxynucleoside triphosphates, at least one chain terminating agent, and a T7-type DNA polymerase having less than 500 units of exonuclease activity under conditions in which the primer is extended until the chain terminating agent is incorporated, and separating the products of the incubating step according to size, whereby at least a part of the nucleotide base sequence of the nucleic acid molecule can be determined.

    摘要翻译： 本发明涉及确定脱氧核糖核酸分子的核苷酸碱基序列的方法，包括以下步骤：使核酸分子与长度为5-8个碱基的寡核苷酸引物一起孵育，多个脱氧核苷三磷酸，至少一个链终止剂 以及T7型DNA聚合酶，其中引物延伸直到链终止剂掺入的条件下具有小于500单位的外切核酸酶活性，并且根据大小分离孵育步骤的产物，由此至少部分 可以确定核酸分子的核苷酸碱基序列。

    Method for nucleic acid hybridization using single-stranded DNA binding protein
    8.
    发明授权
    Method for nucleic acid hybridization using single-stranded DNA binding protein 失效
    使用单链DNA结合蛋白进行核酸杂交的方法

    公开(公告)号：US5534407A

    公开(公告)日：1996-07-09

    申请号：US229396

    申请日：1994-04-18

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/6832 ,  C12Q1/6813

    摘要： Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

    摘要翻译： 使用核酸探针检测不同核酸序列群体中特定核酸序列的存在的核酸杂交方法。 核酸探针与特定核酸序列杂交，但不与群体中的其他核酸序列杂交。 该方法包括在杂交条件下，在单链DNA结合蛋白存在下将样品（可能包括核酸序列）与核酸探针接触，所述单链DNA结合蛋白以刺激稀溶液复性的量提供（即，其中 大于500倍（即，t1 / 2小于60分钟，优选小于5分钟，最优选约1分钟）的单链DNA的复性的t1 / 2长于3周） 缺乏三磷酸核苷酸。

    DNA Sequencing apparatus
    10.
    发明授权
    DNA Sequencing apparatus 失效
    DNA测序装置

    公开(公告)号：US5122345A

    公开(公告)日：1992-06-16

    申请号：US547870

    申请日：1990-07-03

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12Q1/42 ,  C12Q1/48 ,  C12Q1/68

    CPC分类号： C12Q1/6869 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/808 ,  Y10T436/143333

    摘要： An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position anThis invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.