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公开(公告)号：US3806416A
公开(公告)日：1974-04-23
申请号：US24958972
申请日：1972-05-02
发明人： MOLLERING H , BEAUCAMP K , NELBOECK HOCHSTETTER M , BERGMEYER H
CPC分类号： C12Q1/34 , C12N9/78 , C12N9/86 , C12Q1/50 , G01N2333/9123 , G01N2333/978 , G01N2333/986 , Y10S435/815 , Y10S435/816 , Y10S435/824 , Y10S435/829 , Y10S435/933
摘要： CREATININE AMIDOHYDROLASE AND CREATINE AMIDINOHYDROLASE ARE PREPARED BY A PROCESS COMPRISING CULTURING A MICROORGANISM IN A CREATININE-CONTAINING MEDIUM, DIGESTING SAME, OBTAINING THE CREATININE AMIDOHYDROLASE IN SUBSTANTIALLY PURE FORM FROM THE WATER-SOLUBLE DIGESTION FRACTION AND, OPTIONALLY SEPARATING BY ION EXCHANGE CHROMATOGRAPHY ANY CREATINE AMIDINOHYDROLASE PRESENT
摘要翻译： 肌酸酐通过使用新的酶，肌酸酐酰胺水解酶来确定，肌酸酐酰胺水解酶将含肌酸酐样品中的肌酸酐转化为肌酸，后者以常规方式作为肌酸酐原始存在的量度测定。 该酶将肌酸酐转化为肌酸的能力也可用于监测浓缩过程对收集酶的功效，通过对该过程的每个步骤的产物进行取样，并确定其将加入其中的肌酸酐转化为肌酸的能力，如 这种产品中存在的酶的活性的量度。
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2.发明授权
公开(公告)号：US3838011A
公开(公告)日：1974-09-24
申请号：US26176372
申请日：1972-06-12
发明人： HAGEN A , BERGMEYER H , GRUBER W , BEAUCAMP K , JAWOREK D
IPC分类号： G01N27/48 , B01L99/00 , C12Q1/00 , G01N33/487 , G01N31/14
CPC分类号： B01L3/0293 , C12Q1/00 , C12Q1/001 , Y10T137/86775 , Y10T436/117497
摘要： ENZYMATICALLY REACTIVE SUBSTANCES CONTAINED IN AQUEOUS SOLUTIONS ARE QUANTITATIVELY DETERMINED BY INTRODUCING A DEFINED AMOUNT OF A SOLUTION SAMPLE CONTAINING THE SUBSTANCE TO BE DETERMINED AND WHICH IS FREE OF AIR BUBBLES, INTO A STREAM OF BUFFER SOLUTION IN A BLOCK-LIKE FASHION, WHEREIN THE BUFFER SOLUTION CONTAINS THE REAGENTS NECESSARY FOR AN ENZYMATIC REACTION WITH SAID SUBSTANCES AND FLOWS WITH CONSTANT VELOCITY AND WHEREIN THE INTRODUCTION OF THE BLOCK SAMPLE DOES NOT SUBSTANTIALLY ALTER THE VELOCITY OF FLOW, CONTACTING THE RESULTING STREAM WITH THE APPROPRIATE ENZYME AND THEN PASSING THE STREAM OVER A DETECTOR TO INDICATE THE AMOUNT OF REACTION HAVING OCCURED, AND RELATING READINGS FROM THE DETECTOR TO THE AMOUNT OF THE SUBSTANCE TO BE DETERMINED CONTAINED IN THE SAMPLE.
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公开(公告)号：US3749709A
公开(公告)日：1973-07-31
申请号：US3749709D
申请日：1969-03-11
发明人： NELBOECK HOCHSTETTER M , BEAUCAMP K
CPC分类号： C07H19/16
摘要： PROCESS FOR THE CHROMATOGRAPHIC PURIFICATION OF REDUCED PYRIDINE COENZYMES WHICH COMPRISES UTILIZING COLUMNS CHARGED WITH WEAKLY BASIC ANION EXCHANGE RESINS WHICH ARE NOT PRESENT IN THE HYDROXYL FORM AND CARRYING OUT THE ELUTION WITH AN ALKALINE ELUTION AGENT HAVING A PH GREATER THAN 7.5.
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公开(公告)号：US3819487A
公开(公告)日：1974-06-25
申请号：US18164071
申请日：1971-09-17
发明人： BERNT E , BERGMEYER H , BEAUCAMP K , GRASSL M , GRUBER W
IPC分类号： B01D46/52 , C07H19/207 , C12Q1/00 , G01N31/14
CPC分类号： C12Q1/008 , C07H19/207
摘要： Storage stable reduced nicotinamide-adenine-dinucleotide (NADH) compositions, which do not form dehydrogenase inhibitors of prolonged time periods, are produced by forming a dilute aqueous solution of inhibitor-free NADH and maintaining this solution in a thin layer vacuum.
摘要翻译： 通过形成稀释的不含抑制剂的NADH的水溶液并将该溶液保持在薄层真空中来制备不延长时间段的脱氢酶抑制剂的储存稳定的还原型烟酰胺 - 腺嘌呤二核苷酸（NADH）组合物。
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5.发明授权Carrier-bound enzyme prepared by reacting an enzyme with a compound containing an epoxy group and an unsaturated double bond and then polymerizing with an olefinic monomer 失效通过用包含环氧基团和不饱和双键的化合物反应酶，然后与烯烃单体聚合制备的载体环化酶
公开(公告)号：US3806417A
公开(公告)日：1974-04-23
申请号：US25896872
申请日：1972-06-02
发明人： NELBOECK HOCHSTETTER M , BEAUCAMP K , BERGMEYER H , BOTSCH K , JAWOREK D
IPC分类号： C08H1/00 , C08F289/00 , C12N11/08 , C07G7/02
CPC分类号： C08F289/00 , C12N11/08 , Y10S530/815
摘要： CARRIER-BOUND PROTEINS ARE PREPARED BY REACTING A PROTEIN IN AQUEOUS SOLUTION WITH A COMPOUND HAVING AT LEAST ONE EPOXY GROUP AND AT LEAST ONE ADDITIONAL FUNCTIONAL GROUP CAPABLE OF PRODUCING A BOND WITH A CARRIER MATERIAL, AND REACTING THE RESULTING PRODUCT WITH A CARRIER MATERIAL, OPTIONALLY FORMING THE CARRIER MATERIAL IN SITU BY POLYMERIZATION OF A MONOMER OR MONOMER MIXTURE.
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