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1.发明授权
公开(公告)号：US3838011A
公开(公告)日：1974-09-24
申请号：US26176372
申请日：1972-06-12
发明人： HAGEN A , BERGMEYER H , GRUBER W , BEAUCAMP K , JAWOREK D
IPC分类号： G01N27/48 , B01L99/00 , C12Q1/00 , G01N33/487 , G01N31/14
CPC分类号： B01L3/0293 , C12Q1/00 , C12Q1/001 , Y10T137/86775 , Y10T436/117497
摘要： ENZYMATICALLY REACTIVE SUBSTANCES CONTAINED IN AQUEOUS SOLUTIONS ARE QUANTITATIVELY DETERMINED BY INTRODUCING A DEFINED AMOUNT OF A SOLUTION SAMPLE CONTAINING THE SUBSTANCE TO BE DETERMINED AND WHICH IS FREE OF AIR BUBBLES, INTO A STREAM OF BUFFER SOLUTION IN A BLOCK-LIKE FASHION, WHEREIN THE BUFFER SOLUTION CONTAINS THE REAGENTS NECESSARY FOR AN ENZYMATIC REACTION WITH SAID SUBSTANCES AND FLOWS WITH CONSTANT VELOCITY AND WHEREIN THE INTRODUCTION OF THE BLOCK SAMPLE DOES NOT SUBSTANTIALLY ALTER THE VELOCITY OF FLOW, CONTACTING THE RESULTING STREAM WITH THE APPROPRIATE ENZYME AND THEN PASSING THE STREAM OVER A DETECTOR TO INDICATE THE AMOUNT OF REACTION HAVING OCCURED, AND RELATING READINGS FROM THE DETECTOR TO THE AMOUNT OF THE SUBSTANCE TO BE DETERMINED CONTAINED IN THE SAMPLE.
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公开(公告)号：US3819487A
公开(公告)日：1974-06-25
申请号：US18164071
申请日：1971-09-17
发明人： BERNT E , BERGMEYER H , BEAUCAMP K , GRASSL M , GRUBER W
IPC分类号： B01D46/52 , C07H19/207 , C12Q1/00 , G01N31/14
CPC分类号： C12Q1/008 , C07H19/207
摘要： Storage stable reduced nicotinamide-adenine-dinucleotide (NADH) compositions, which do not form dehydrogenase inhibitors of prolonged time periods, are produced by forming a dilute aqueous solution of inhibitor-free NADH and maintaining this solution in a thin layer vacuum.
摘要翻译： 通过形成稀释的不含抑制剂的NADH的水溶液并将该溶液保持在薄层真空中来制备不延长时间段的脱氢酶抑制剂的储存稳定的还原型烟酰胺 - 腺嘌呤二核苷酸（NADH）组合物。
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公开(公告)号：US3721607A
公开(公告)日：1973-03-20
申请号：US3721607D
申请日：1971-08-11
发明人： GRUBER W , BERNT E , GAWEHN K , BERGMEYER H , WERNER W
CPC分类号： C12Q1/54 , C12Q1/28 , C12Q2326/30
摘要： Compositions comprising glucose oxidase, peroxidase, a chromogen, a buffer, an azide, and 2,2''-azino-di-(3ethylbenzothiazoline-6-sulfonic acid) provide remarkably stable test reagents for the enzymatic determination of glucose.
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公开(公告)号：US3775253A
公开(公告)日：1973-11-27
申请号：US3775253D
申请日：1972-06-15
发明人： JAWOREK D , GRUBER W , BERGMEYER H
IPC分类号： C08F220/00 , A61K39/44 , C08F8/00 , C08F20/52 , C08F20/56 , C08F22/00 , C08F220/04 , C08G81/00 , C08G81/02 , C12N11/08 , G01N33/545 , C07G7/00 , C07G7/02
CPC分类号： C12N11/08 , A61K39/44 , C08F8/00 , C08F20/56 , C08F22/00 , Y10S530/815 , Y10S530/816 , C08F220/56 , C08F222/00
摘要： Water insoluble biologically active proteins are bound on novel copolymers consisting of (a) acrylamide (b) ethylene-maleic acid or its anhydride and/or (c) maleic acid and/or its anhydride and (d) N,N''-methylene-bis-acrylamide or ethylene diacrylate in a weight ratio a:b:c:d of 3 : 0.5 - 1.5 : 0.05 - 4 : 0.075 - 0.9, the weight ratio a : (b + c) being not more than 4, to provide compositions which are outstanding in their ability to yield up the bound protein, and with which precise substrate reactions can be carried out.
摘要翻译： 水不溶性生物活性蛋白质结合在由（a）丙烯酰胺（b）乙烯 - 马来酸或其酸酐和/或（c）马来酸和/或其酸酐和（d）N，N'-亚甲基 - 双丙烯酰胺或乙烯二丙烯酸酯的重量比a:b:c:d为3:0.5-1.5:0.05-4:0.075-0.9，重量比a:（b + c）不大于4，以提供 在产生结合蛋白的能力方面是突出的，并且可以进行精确的底物反应的组合物。
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5.发明授权
公开(公告)号：US3762609A
公开(公告)日：1973-10-02
申请号：US3762609D
申请日：1972-06-12
发明人： HAGEN A , MEWES D , GRUBER W , BERGMEYER H
CPC分类号： G01N35/1097 , G05D11/001 , G05D11/003
摘要： A sample to be analyzed is injected into a stream of carrier liquid by coupled dual valves in two branches of the stream. One of the two valves is open and the other closed at any one time. The bore of the closed valve provides a metering cavity which is filled with the sample to be analyzed, and the sample is injected by simultaneously closing the previously open valve and opening the sample-bearing closed valve. The specific, dual valve arrangement disclosed has a single movable disc arranged between two stationary discs, the discs being provided with passages filled and emptied in the necessary sequence when the movable disc is rotated.
摘要翻译： 待分析的样品通过在流的两个分支中的耦合双阀注入载液流中。 两个阀门中的一个是打开的，另一个在任何一个时间都关闭。 关闭阀的孔提供了一个填充有待分析样品的计量腔，并且通过同时关闭先前打开的阀并打开带样本的封闭阀注入样品。 所公开的具体的双阀装置具有设置在两个固定盘之间的单个可移动盘，当可移动盘旋转时，盘设置有以必要顺序填充和排空的通道。
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公开(公告)号：US3791931A
公开(公告)日：1974-02-12
申请号：US3791931D
申请日：1971-11-30
发明人： BERGMEYER H , MOLLERING H , WEIMANN G , NAHER G , GRUBER W , THUM W , WAHLEFELD A
CPC分类号： C12Q1/32 , C12Q1/26 , Y10S435/842
摘要： Lactate dehydrogenase is quickly and reliably determined by means of a color test by contacting the test composition with the reagent comprising serum albumin, pig''s heart diaphorase and nicotinamide-adenine-dinucleotide, wherein the diaphorase is preferably obtained from the protein fraction of pig''s heart insoluble in 1.6 to 2.8M ammonium sulphate by treatment with 0.1 to 0.3 percent weight/volume polyethyleneimine, heating at 70* to 80* C., adsorption on a weakly acidic cation exchanger and subsequent elution.
摘要翻译： 通过使测试组合物与包含血清白蛋白，猪心脏肌苷酸和烟酰胺 - 腺嘌呤二核苷酸的试剂接触，通过颜色测试快速可靠地测定乳酸脱氢酶，其中，心肌黄酶优选地从不溶于猪心脏的蛋白质级分中获得 1.6至2.8M硫酸铵，用0.1至0.3％重量/体积的聚乙烯亚胺处理，加热至70℃至80℃，在弱酸性阳离子交换剂上吸附并随后洗脱。
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公开(公告)号：US3778350A
公开(公告)日：1973-12-11
申请号：US3778350D
申请日：1970-08-05
发明人： BERGMEYER H , SCHMIDT F , STORK H , BERNT E , GRUBER W
CPC分类号： C12Q1/54
摘要： GLUCOSE IS DETERMINED ENZYMATICALLY WITH A STABLE DIAGNOSTIC AGENT MIXTURE COMPRISING HEXOLKINASE, GLUCOSE-6PHOSPHATE DEHYDROGENASE, ATP, MAGNESIUM SULFATE, NADP. AND A BUFFER CONTAINING AN AZIDE.
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公开(公告)号：US3754862A
公开(公告)日：1973-08-28
申请号：US3754862D
申请日：1972-02-29
发明人： WAHLEFELD A , BERNT E , GRUBER W , BERGMEYER H
CPC分类号： G01N33/728 , Y10S436/903 , Y10T436/146666
摘要： Total bilirubin is determined with a stable, accurate, widerange reagent composition comprising: A. AT LEAST ONE OF THE GROUP OF CHLOROPHENYL DIAZONIUM CHLORIDES CONSISTING OF 2,4-DICHLORO-, 2,5-DICHLORO- OR 2-CHLORO-4-NITROPHENYL-DIAZONIUM CHLORIDE AND, B. AN ALKYL-PHENOL POLYETHYLENE OXIDE; TO PRODUCE A COLOR WHICH IS THEN TAKEN AS A MEASURE OF BILIRUBIN CONTENT.
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公开(公告)号：US3838065A
公开(公告)日：1974-09-24
申请号：US27911672
申请日：1972-08-09
发明人： LIPPERT W , BERNT E , GRUBER W , BERGMEYER H
CPC分类号： G01N33/96 , Y10T436/10 , Y10T436/108331
摘要： A STABILIZED GLYCEROL STANDARD SOLUTION, CONSISTING ESSENTIALLY OF GLYCEROL IN 0.02 TO 0.2 N PERCHLORIC ACID, IS ADVANTAGEOUSLY USED TO PROVIDE A REFERENCE STANDARD IN ANALYTICAL METHODS FOR THE DETERMINATION OF GLYCEROL.
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10.发明授权Reagent composition and process for determining gamma-glutamyltranspeptidase 失效试剂组合物和测定GAMMA-谷氨酰胺磷酸酶的方法
公开(公告)号：US3773626A
公开(公告)日：1973-11-20
申请号：US3773626D
申请日：1971-08-11
发明人： BERNT E , BERGMEYER H , GRUBER W , LIPPERT W
CPC分类号： C12Q1/48
摘要： A composition comprising gamma -glutamyl-p-nitroaniline, glucyl-glycine, a buffer, a surface-active agent and polyvinylpyrrolidone is an exceptionally stable reagent for determining gamma -glutamyl-transpeptidase.
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