公开(公告)号：US07132081B2

公开(公告)日：2006-11-07

申请号：US10296056

申请日：2001-04-27

申请人： Beate Diefenbach ,  Holger Deppe ,  Hanns Wurziger ,  Alexander Gross ,  Gregor Schlingloff ,  Andreas Schober ,  Dirk Tomandl

发明人： Beate Diefenbach ,  Holger Deppe ,  Hanns Wurziger ,  Alexander Gross ,  Gregor Schlingloff ,  Andreas Schober ,  Dirk Tomandl

IPC分类号： B01L3/00

CPC分类号： B01L3/50853 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00333 ,  B01J2219/00585 ,  B01L3/50857 ,  B01L2300/0819 ,  C40B60/14

摘要： Microwells, which are open on both sides and are arranged two-dimensionally in a microwell matrix (4), are hermetically closed by pressure on both sides using plugs (6) covered with a sealing sheet (11). A layer of elastic material (9), which is arranged between the plug (6) and the sealing sheet (11), guarantees uniform pressure on all the microwells of a microwell matrix (4) which is loosely held by a frame structure (1). Continuous peripheral elevations (2), which protrude in both directions at right angles to the plane of the microwell matrix (4), ensure that the deformable thin layer of elastic material (9) does not escape sideways between the plug (6) and the microwell matrix (4) during the pressing process. A plurality of microwell matrices (4) are held simultaneously by the frame structure (1) and are hermetically closed by a corresponding number of allocated plugs (6), which are fastened to a base plate (7) and a cover plate (8), respectively.

摘要翻译： 在两侧开口并且在微孔矩阵（4）中二维布置的微孔通过用密封片（11）覆盖的塞子（6）在两侧通过压力气密封闭。 布置在塞子（6）和密封片（11）之间的一层弹性材料（9）保证了均匀的压力，所述的微孔基体（4）的所有微孔被框架结构（1）松动地保持 ）。 在与微孔矩阵（4）的平面成直角的两个方向上突出的连续周边高度（2）确保弹性材料（9）的可变形薄层不会在插头（6）和 微孔基质（4）。 多个微孔矩阵（4）由框架结构（1）同时保持，并被相应数量的分配的塞子（6）气密地封闭，该插头固定到基板（7）和盖板（8）上， ， 分别。

公开(公告)号：US08703039B2

公开(公告)日：2014-04-22

申请号：US13497814

申请日：2010-02-24

申请人： Andreas Schober ,  Alexander Gross ,  Joerg Hampl

发明人： Andreas Schober ,  Alexander Gross ,  Joerg Hampl

IPC分类号： B29C51/14

CPC分类号： B29C49/0047 ,  B29C2791/006 ,  B29C2791/007 ,  B29K2027/12 ,  B29L2031/756

摘要： A microstructured composite component is provided including structures for carrying out fluid processes. A device is provided for producing microstructured composite components. A method provided includes a first step during which a first film is arranged above a second film between first and second mold parts. The first and/or second mold parts have microstructured cavities to be filled. In a second step, the first and second mold parts are heated in at least one region in which they contact one of the films. An excess pressure is then produced between the first film and the second film to force the first and/or second films into the cavities. In a further step, the first and second mold parts are pressed together by a pressing force. Once the first mold part, the second mold part, the first film and the second film are cooled, they form a microstrutured composite component.

摘要翻译： 提供了一种微结构化复合材料组件，包括用于进行流体过程的结构。 提供了用于生产微结构化复合材料组件的装置。 提供的方法包括第一步骤，在第一步骤中，第一薄膜布置在第一和第二模具部件之间的第二薄膜之上。 第一和/或第二模具部件具有要填充的微结构化空腔。 在第二步骤中，第一和第二模具部件在至少一个它们接触薄膜之一的区域中被加热。 然后在第一膜和第二膜之间产生过量的压力，以迫使第一和/或第二膜进入空腔。 在另一步骤中，第一和第二模具部件通过按压力被压在一起。 一旦第一模具部件，第二模具部件，第一薄膜和第二薄膜被冷却，它们形成微导体复合部件。

公开(公告)号：US20120231226A1

公开(公告)日：2012-09-13

申请号：US13497814

申请日：2010-02-24

申请人： Andreas Schober ,  Alexander Gross ,  Joerg Hampl

发明人： Andreas Schober ,  Alexander Gross ,  Joerg Hampl

IPC分类号： B32B3/10 ,  B32B37/10 ,  B32B37/06 ,  B32B37/02

CPC分类号： B29C49/0047 ,  B29C2791/006 ,  B29C2791/007 ,  B29K2027/12 ,  B29L2031/756

摘要： A microstructured composite component is provided including structures for carrying out fluid processes. A device is provided for producing microstructured composite components. A method provided includes a first step during which a first film is arranged above a second film between first and second mold parts. The first and/or second mold parts have microstructured cavities to be filled. In a second step, the first and second mold parts are heated in at least one region in which they contact one of the films. An excess pressure is then produced between the first film and the second film to force the first and/or second films into the cavities. In a further step, the first and second mold parts are pressed together by a pressing force. Once the first mold part, the second mold part, the first film and the second film are cooled, they form a microstrutured composite component.

摘要翻译： 提供了一种微结构化复合材料组件，包括用于进行流体过程的结构。 提供了用于生产微结构化复合材料组件的装置。 提供的方法包括第一步骤，在第一步骤中，第一薄膜布置在第一和第二模具部件之间的第二薄膜之上。 第一和/或第二模具部件具有要填充的微结构化空腔。 在第二步骤中，第一和第二模具部件在至少一个它们接触薄膜之一的区域中被加热。 然后在第一膜和第二膜之间产生过量的压力，以迫使第一和/或第二膜进入空腔。 在另一步骤中，第一和第二模具部件通过按压力被压在一起。 一旦第一模具部件，第二模具部件，第一薄膜和第二薄膜被冷却，它们形成微导体复合部件。

公开(公告)号：US20120079895A1

公开(公告)日：2012-04-05

申请号：US13377632

申请日：2010-02-24

申请人： Andreas Schober ,  G. Alexander Gross ,  Michael Kohler

发明人： Andreas Schober ,  G. Alexander Gross ,  Michael Kohler

IPC分类号： G01N35/08 ,  G01N21/00

CPC分类号： G01N35/08

摘要： The invention relates to a device for generating and/or arranging sequences of a fluid sample in a carrier fluid. The invention comprises a microchannel having an inlet, an outlet and a nozzle opening therebetween leading into the microchannel. The invention further relates to a delivery unit, which pumps in the carrier fluid using a feed volume flow and suctions off the carrier fluid using a discharge volume flow. In a sample container, the nozzle opening is in contact with the fluid sample. By means of a control unit, the ratio between feed volume flow and discharge volume flow is varied. The cross-section of the nozzle opening is selected such that no carrier medium exits from the nozzle opening when the feed volume flow equals the discharge volume flow and that a fluid sample enters the nozzle opening when the discharge volume flow is greater than the feed volume flow. The invention further relates to a method for generating and/or arranging sequences of a fluid sample in a carrier fluid.

摘要翻译： 本发明涉及用于在载体流体中产生和/或排列流体样品序列的装置。 本发明包括具有进入微通道的入口，出口和喷嘴开口的微通道。 本发明还涉及一种输送单元，其使用进料体积流泵送在载体流体中，并且使用排出体积流吸收载体流体。 在样品容器中，喷嘴开口与流体样品接触。 通过控制单元，进料体积流量和排出体积流量之间的比率是变化的。 选择喷嘴开口的横截面，使得当进料体积流量等于排出体积流量时，没有载体介质从喷嘴开口离开，并且当排出体积流量大于进料体积时流体样品进入喷嘴开口 流。 本发明还涉及用于在载体流体中产生和/或排列流体样品序列的方法。

公开(公告)号：US4172117A

公开(公告)日：1979-10-23

申请号：US838332

申请日：1977-09-30

申请人： Andreas Schober

发明人： Andreas Schober

IPC分类号： G01N33/543 ,  G01N23/00 ,  G01N33/16 ,  G01T1/16

CPC分类号： G01N33/54306 ,  Y10S436/804 ,  Y10S436/819 ,  Y10S436/82

摘要： Antigens and their antibodies are measured in a combined radioimmunoassay by incubating a sample of an unknown fluid with a known quantity of an antibody, contacting the mixture obtained with the walls of a vessel having a coating of known antigen content, again incubating, then aspirating the mixture and washing the walls of the vessel, adding a pure radioactive tracer antigen, aspirating and washing again, and finally measuring the radioactivty of the coated parts conventionally, e.g., in a gamma counter known in the art.

摘要翻译： 通过将未知流体的样品与已知量的抗体一起孵育来测量抗原及其抗体，将获得的混合物与具有已知抗原含量的涂层的血管壁接触，再次孵育， 混合物并洗涤容器的壁，加入纯的放射性示踪剂抗原，再次抽吸和洗涤，最后通常例如在本领域已知的γ计数器中测量涂覆部件的放射性。

公开(公告)号：US20130197668A1

公开(公告)日：2013-08-01

申请号：US13877721

申请日：2011-09-30

申请人： Andreas Schober ,  Michael Gebinoga ,  Uta Fernekorn ,  Frank Weise ,  Jörg Hampl ,  Julia Katzmann ,  Thomas Klar

发明人： Andreas Schober ,  Michael Gebinoga ,  Uta Fernekorn ,  Frank Weise ,  Jörg Hampl ,  Julia Katzmann ,  Thomas Klar

IPC分类号： A61F2/02

CPC分类号： A61F2/02 ,  G05B19/4099 ,  G05B2219/49019 ,  G05B2219/49031 ,  G06F19/12 ,  G06F19/24

摘要： The present invention relates to a method for producing a structure modeled on a biological tissue. The invention also relates to a structure which can be produced using the method according to the invention. According to an embodiment of the invention, a precursor of a biopolymer is locally irradiated with electromagnetic radiation in a targeted manner, wherein the irradiation, in particular the selection of the areas to be irradiated, is effected according to data which describe a structural construction at least components of the extracellular matrix of the biological tissue. In this case, the electromagnetic radiation is such that two-photon or multi-photon absorption takes place in the irradiated areas of the precursor and results in the precursor being polymerized to form the biopolymer in the irradiated areas, with the result being that the precursor is at least partially solidified there.

摘要翻译： 本发明涉及一种用于生产以生物组织为模型的结构的方法。 本发明还涉及可以使用根据本发明的方法制造的结构。 根据本发明的一个实施方案，生物聚合物的前体以目标方式用电磁辐射局部照射，其中照射，特别是对待照射区域的选择，是根据描述在 生物组织的细胞外基质的最少组分。 在这种情况下，电磁辐射使得在前体的照射区域中发生双光子或多光子吸收，并导致前体聚合以在照射区域中形成生物聚合物，结果是前体 在那里至少部分固化。

公开(公告)号：US09375305B2

公开(公告)日：2016-06-28

申请号：US13877721

申请日：2011-09-30

申请人： Andreas Schober ,  Michael Gebinoga ,  Uta Fernekorn ,  Frank Weise ,  Jörg Hampl ,  Julia Katzmann ,  Thomas Klar

发明人： Andreas Schober ,  Michael Gebinoga ,  Uta Fernekorn ,  Frank Weise ,  Jörg Hampl ,  Julia Katzmann ,  Thomas Klar

IPC分类号： G01N33/48 ,  G01N33/50 ,  A61F2/02 ,  G05B19/4099 ,  G06F19/24 ,  G06F19/12

CPC分类号： A61F2/02 ,  G05B19/4099 ,  G05B2219/49019 ,  G05B2219/49031 ,  G06F19/12 ,  G06F19/24

摘要： The present invention relates to a method for producing a structure modeled on a biological tissue. The invention also relates to a structure which can be produced using the method according to the invention. According to an embodiment of the invention, a precursor of a biopolymer is locally irradiated with electromagnetic radiation in a targeted manner, wherein the irradiation, in particular the selection of the areas to be irradiated, is effected according to data which describe a structural construction at least components of the extracellular matrix of the biological tissue. In this case, the electromagnetic radiation is such that two-photon or multi-photon absorption takes place in the irradiated areas of the precursor and results in the precursor being polymerized to form the biopolymer in the irradiated areas, with the result being that the precursor is at least partially solidified there.

摘要翻译： 本发明涉及一种用于生产以生物组织为模型的结构的方法。 本发明还涉及可以使用根据本发明的方法制造的结构。 根据本发明的一个实施方案，生物聚合物的前体以目标方式用电磁辐射局部照射，其中照射，特别是对待照射区域的选择，是根据描述在 生物组织的细胞外基质的最少组分。 在这种情况下，电磁辐射使得在前体的照射区域中发生双光子或多光子吸收，并导致前体聚合以在照射区域中形成生物聚合物，结果是前体 在那里至少部分固化。

公开(公告)号：US5065154A

公开(公告)日：1991-11-12

申请号：US340981

申请日：1989-04-20

申请人： Winfried Kaiser ,  Andreas Schober ,  E.-Peter Salfeld

发明人： Winfried Kaiser ,  Andreas Schober ,  E.-Peter Salfeld

IPC分类号： G06F13/14 ,  G06F12/06 ,  H04Q9/14

CPC分类号： G06F12/0676

摘要： In a system comprising a plurality of digitally addressable electronic devices, all devices have the same construction and are designed for being connected in series to a common central monitoring station. The addressing of these devices is accomplished by a plurality of address lines which are connected to input contacts of the respective device. Within each device, a selection gate is connected to the input contacts which provides an enabling signal if the signal on said address lines has a predetermined bit pattern. Each device has output contacts connected to its input contacts to which the input contacts of a next device are connected. The address lines are connected from the input contacts to the output contacts of the device in such a way that the contacts are interchanged, and an inverter is inserted in one of said lines so that the bit pattern forming the address provided at the output contacts is different from the bit pattern received at the input contacts. Thus, along the system of series connected devices a plurality of different bit patterns is produced so that the position of the correct bit pattern to which the device selection means responds, is shifted along the series connected devices in order to address the device which is in a predetermined position. A preferred application of such devices is in medical surveillance systems in which a plurality of racks are connected serially together and to a center monitoring station, each rack being allocated to a different task and including the units which are needed to monitor the vital functions of a patient required for that task.

公开(公告)号：US20120319705A1

公开(公告)日：2012-12-20

申请号：US13581060

申请日：2011-02-23

申请人： Andreas Schober ,  Jörg Hampl ,  Uta Fernekorn ,  Peter Husar ,  Michael Fischer ,  Daniel Laqua ,  Katharina Lilienthal

发明人： Andreas Schober ,  Jörg Hampl ,  Uta Fernekorn ,  Peter Husar ,  Michael Fischer ,  Daniel Laqua ,  Katharina Lilienthal

IPC分类号： G01R19/00 ,  G01R27/26

CPC分类号： A61N1/05 ,  A61B5/04001 ,  A61B5/685 ,  A61B2562/028 ,  A61B2562/046 ,  A61N1/0529

摘要： The invention relates to a hybrid three-dimensional sensor array, in particular for measuring biological cell assemblies. The sensor array has a plurality of microstructured sensor plates, each having one carrier section on which a plurality of sensor needles are arranged in a comb-like manner, which carry a plurality of electrode surfaces. Furthermore, a plurality of spacer elements are provided, which are fastened between the sensor plates so that both the carrier sections and the sensor needles of adjacent sensor plates are at a distance from each other. The invention further relates to a measuring assembly for measuring electrical activities of biological cell assemblies using such a sensor array.

摘要翻译： 本发明涉及一种混合三维传感器阵列，特别是用于测量生物细胞组件。 传感器阵列具有多个微结构传感器板，每个具有一个载体部分，多个传感器针以梳状布置在该载体部分上，该传感器带有多个电极表面。 此外，设置有多个间隔元件，其被固定在传感器板之间，使得相邻传感器板的载体部分和传感器针头彼此相隔一定距离。 本发明还涉及一种用于使用这种传感器阵列测量生物细胞组件的电活动的测量组件。

公开(公告)号：US20110130310A1

公开(公告)日：2011-06-02

申请号：US12675225

申请日：2009-03-31

申请人： Andreas Schober ,  Caroline Augspurger ,  Frank Weise ,  Uta Fernekorn ,  Christian Hildmann ,  Jorg Hampl

发明人： Andreas Schober ,  Caroline Augspurger ,  Frank Weise ,  Uta Fernekorn ,  Christian Hildmann ,  Jorg Hampl

IPC分类号： C40B60/12 ,  C12M3/00 ,  C12M1/34

CPC分类号： F04B43/046 ,  B01L3/502715 ,  B01L3/50857 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2400/0487 ,  B01L2400/0638 ,  C12M23/16

摘要： The present invention relates to a microbioreactor comprising a sample support (7) for holding cells; a bypass (3) with a capillary opening for feeding test reagent and/or for aeration and ventilation of the microbioreactor; a pump module (5) for perfusion of the microbioreactor with test reagent and/or gas feeding and purging of the microbioreactor; a transparent viewing window (2); and fluid channels (4) that form a circuit. The invention further relates to a bioreactor-microtiter plate comprising a plurality of such microbioreactors.

摘要翻译： 本发明涉及包含用于保持细胞的样品支持体（7）的微生物反应器; 旁路（3），其具有用于供给测试试剂的毛细管孔和/或用于微生物反应器的通气和通风的旁路（3）; 泵组件（5），用于用微生物反应器的测试试剂和/或气体供给和清洗灌注微生物反应器; 透明的观察窗（2）; 和形成电路的流体通道（4）。 本发明还涉及包含多个这样的微生物反应器的生物反应器 - 微量滴定板。