-
公开(公告)号：US08247192B2
公开(公告)日：2012-08-21
申请号：US12615139
申请日：2009-11-09
申请人： Behnaz Behrouzian , Anke Krebber , Emily Mundorff
发明人： Behnaz Behrouzian , Anke Krebber , Emily Mundorff
CPC分类号： C12N9/84 , C12P7/22 , C12P37/06 , C12P41/004
摘要： The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
摘要翻译： 本公开涉及具有改进性质的工程青霉素G酰基转移酶（PGA）酶，编码这些酶的多核苷酸，包含酶的组合物和使用酶的方法。
-
公开(公告)号：US08569013B2
公开(公告)日：2013-10-29
申请号：US13542835
申请日：2012-07-06
申请人： Behnaz Behrouzian , Anke Krebber , Emily Mundorff
发明人： Behnaz Behrouzian , Anke Krebber , Emily Mundorff
CPC分类号： C12N9/84 , C12P7/22 , C12P37/06 , C12P41/004
摘要： The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
摘要翻译： 本公开涉及具有改进性质的工程青霉素G酰基转移酶（PGA）酶，编码这些酶的多核苷酸，包含酶的组合物和使用酶的方法。
-
公开(公告)号：US20120270282A1
公开(公告)日：2012-10-25
申请号：US13542835
申请日：2012-07-06
申请人： Behnaz Behrouzian , Anke Krebber , Emily Mundorff
发明人： Behnaz Behrouzian , Anke Krebber , Emily Mundorff
CPC分类号： C12N9/84 , C12P7/22 , C12P37/06 , C12P41/004
摘要： The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
摘要翻译： 本公开涉及具有改进性质的工程青霉素G酰基转移酶（PGA）酶，编码这些酶的多核苷酸，包含酶的组合物和使用酶的方法。
-
公开(公告)号：US08614081B2
公开(公告)日：2013-12-24
申请号：US13381155
申请日：2010-07-22
申请人： Anke Krebber , Emily Mundorff , Marissa Mock , Spiros Kambourakis
发明人： Anke Krebber , Emily Mundorff , Marissa Mock , Spiros Kambourakis
CPC分类号： C12N9/78 , C12Y305/05001
摘要： The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
摘要翻译： 本公开涉及具有腈水解酶活性的多肽，编码多肽的多核苷酸以及使用该多肽的方法。
-
公开(公告)号：US08748143B2
公开(公告)日：2014-06-10
申请号：US12210195
申请日：2008-09-13
申请人： Jack Liang , Stephane J. Jenne , Emily Mundorff , Charlene Ching , John M. Gruber , Anke Krebber , Gjalt W. Huisman
发明人： Jack Liang , Stephane J. Jenne , Emily Mundorff , Charlene Ching , John M. Gruber , Anke Krebber , Gjalt W. Huisman
CPC分类号： C12N9/0006 , C12P7/22 , C12Y101/01 , C12Y101/01184 , Y02E50/17 , Y02P20/52
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
-
公开(公告)号：US20090191605A1
公开(公告)日：2009-07-30
申请号：US12210195
申请日：2008-09-13
申请人： Jack Liang , Stephane J. Jenne , Emily Mundorff , Charlene Ching , John M. Gruber , Anke Krebber , Gjalt W. Huisman
发明人： Jack Liang , Stephane J. Jenne , Emily Mundorff , Charlene Ching , John M. Gruber , Anke Krebber , Gjalt W. Huisman
CPC分类号： C12N9/0006 , C12P7/22 , C12Y101/01 , C12Y101/01184 , Y02E50/17 , Y02P20/52
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
摘要翻译： 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。
-
公开(公告)号：US20090118130A1
公开(公告)日：2009-05-07
申请号：US12069869
申请日：2008-02-12
CPC分类号： C12Q1/25 , C12N9/00 , C12N15/1093 , C12Q1/00 , C12Q1/26 , C40B10/00 , C40B30/04 , C40B40/02 , C40B40/10 , G01N33/6803 , G01N33/6845 , G16B20/00
摘要： The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
摘要翻译： 本公开涉及用于筛选多种多肽变体的组合物和方法。
-
公开(公告)号：US07833767B2
公开(公告)日：2010-11-16
申请号：US12576195
申请日：2009-10-08
CPC分类号： C12N9/0006
摘要： The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.
摘要翻译： 本发明涉及具有增强的酮还原酶活性和/或用于酮的立体特异性还原的热稳定性的变体多肽。 此外，本发明涉及编码酮还原酶多肽的多核苷酸，包括提供宿主细胞中增强表达的多核苷酸的密码子优化形式。 在另一方面，本发明涉及用本发明的多核苷酸转化的核苷酸构建体，载体和宿主细胞。
-
9.发明授权Process for the preparation of recombinant proteins in E. coli by high cell density fermentation 有权通过高细胞密度发酵在大肠杆菌中制备重组蛋白的方法公开(公告)号：US06410270B1
公开(公告)日：2002-06-25
申请号：US09077549
申请日：1998-11-16
申请人： Wolfgang Strittmatter , Siegfried Matzku , Dieter Riesenberg , Uwe Horn , Uwe Knüpeer , Marian Kujau , Rolf Wenderoth , Andreas Plückthun , Anke Krebber
发明人： Wolfgang Strittmatter , Siegfried Matzku , Dieter Riesenberg , Uwe Horn , Uwe Knüpeer , Marian Kujau , Rolf Wenderoth , Andreas Plückthun , Anke Krebber
IPC分类号： C12N1500
CPC分类号： C07K16/2863 , C12N15/72 , C12P21/02
摘要： The invention relates to a fed-batch fermentation process which uses special E. coli host/vector systems for the purpose of efficiently forming recombinant proteins, in particular recombinant antibody molecules, preferably antibody fragments such as miniantibodies. Under the given conditions, the E. coli cells are able to grow at a maximum specific growth rate up to very high cell densities. After the recombinant product formation has been switched on, it is only the formed product which restricts growth; there is no growth restriction due to substrates or metabolic by-products. High space-time yields of recombinant proteins can be achieved in this manner.
摘要翻译： 本发明涉及一种补料分批发酵方法，其使用特殊的大肠杆菌宿主/载体系统，以有效形成重组蛋白，特别是重组抗体分子，优选抗体片段，例如微型抗体。 在给定的条件下，大肠杆菌细胞能够以最大比生长速率生长直到非常高的细胞密度。 在重组产物形成开始后，只有限制生长的形成产物; 由于底物或代谢副产物没有生长限制。 可以以这种方式实现重时蛋白质的高空时产量。
-
公开(公告)号：US20100304459A1
公开(公告)日：2010-12-02
申请号：US12790784
申请日：2010-05-28
CPC分类号： C12N9/0006
摘要： The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
摘要翻译： 本发明涉及相对于骨架野生型葡萄糖脱氢酶多肽具有增强的GDH活性和/或热稳定性的葡萄糖脱氢酶（GDH）多肽。 此外，本发明涉及编码本发明的GDH多肽的多核苷酸，包含多核苷酸的核酸序列，包含与启动子有效连接的多核苷酸的表达载体，转化为表达GDH的宿主细胞 多肽，以及本发明的GDH多肽的制备方法。
-
-
-
-
-
-
-
-
-
搜索结果
20 条记录 (耗时 0.02 秒)