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公开(公告)号：US20230372935A1
公开(公告)日：2023-11-23
申请号：US18362530
申请日：2023-07-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz , Erin R. Chia , Billy W. Colston , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC classification number: B01L3/50273 , B01F23/41 , B01F33/813 , B01F33/3011 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/23
Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US11612892B2
公开(公告)日：2023-03-28
申请号：US17110095
申请日：2020-12-02
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. Ness , Donald A. Masquelier , Billy W. Colston, Jr. , Benjamin J. Hindson
IPC: B01L3/00 , B01L3/02 , B01L7/00 , G01N21/3563 , B01F23/41 , B01F33/3011 , B01F35/10 , C12Q1/686 , G01N21/64 , B29C45/00 , G01N21/49 , B01F101/23 , B29L31/00
Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.
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公开(公告)号：US11130134B2
公开(公告)日：2021-09-28
申请号：US16404659
申请日：2019-05-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Billy W. Colston, Jr. , Benjamin J. Hindson , Kevin D. Ness , Donald A. Masquelier
IPC: B01L3/00 , B01F13/00 , B01L3/02 , B01L7/00 , G01N21/3563 , B01F3/08 , C12Q1/686 , G01N21/64 , B01F15/00 , B29C45/00 , G01N21/49 , B29L31/00
Abstract: Method of performing a droplet-based assay. The method may include obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer, performing a reaction in the droplets while packed closely together in the monolayer; and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.
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公开(公告)号：US09822393B2
公开(公告)日：2017-11-21
申请号：US14201752
申请日：2014-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
CPC classification number: C12Q1/6804 , B01L3/502784 , B01L7/52 , C12Q1/6844 , C12Q1/6851 , C12Q2527/125 , C12Q2563/159 , C12Q2537/143
Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US09758535B2
公开(公告)日：2017-09-12
申请号：US14216006
申请日：2014-03-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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Patent Agency Ranking
公开(公告)号：US20170056884A1
公开(公告)日：2017-03-02
申请号：US15351331
申请日：2016-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well.
Abstract translation: 用于形成和浓缩乳液的系统，包括方法，装置和试剂盒。 一个示例性系统可以包括一个装置，该装置包括一个样本阱，其被配置成接收含有样品的流体，一个连续相阱，被配置成接收连续相流体，一个液滴阱以及一个使阱连接的通道网络。 该系统还可以包括被配置为可操作地接收该装置并且产生（i）第一压差以产生在液滴阱中收集的乳液的仪器，和（ii）第二压力差以减小连续相流体的体积分数 在乳液中，在乳液已经收集在液滴中之后，通过相对于含有样品的液滴从液滴中选择性驱动连续相流体。
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公开(公告)号：US20160076099A1
公开(公告)日：2016-03-17
申请号：US14848311
申请日：2015-09-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/68
CPC classification number: C12Q1/6883 , C12Q1/683 , C12Q1/6858 , C12Q2600/158 , C12Q2600/172 , C12Q2537/16 , C12Q2563/159 , C12Q2537/165
Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.
Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以使得样品中核酸的拷贝数估计可以进行。 本文还提供了用于确定样品中靶核酸的两个或多个拷贝（例如两个或多个拷贝是在同一染色体上还是在不同染色体上）或用于定相等位基因的连接的方法，组合物和试剂盒。
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公开(公告)号：US20140200164A1
公开(公告)日：2014-07-17
申请号：US14159410
申请日：2014-01-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Anthony J. Makarewicz, JR. , Amy L. Hiddessen , Kevin D. Ness , Donald. A. Masquelier , Billy W. Colston, JR. , Benjamin J. Hindson , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
IPC: C12Q1/68
CPC classification number: B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
Abstract: System, including methods and apparatus, for detection of spaced droplets.
Abstract translation: 用于检测间隔液滴的系统，包括方法和装置。
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公开(公告)号：US20220008928A1
公开(公告)日：2022-01-13
申请号：US17486804
申请日：2021-09-27
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Billy W. Colston, JR. , Benjamin J. Hindson , Kevin D. Ness , Donald A. Masquelier
IPC: B01L3/00 , B01F13/00 , B01L3/02 , B01L7/00 , G01N21/3563 , B01F3/08 , C12Q1/686 , G01N21/64 , B01F15/00 , B29C45/00 , G01N21/49
Abstract: Method of analysis. In the method, a microfluidic device defining a flow path extending from an inlet to an outlet may be selected. A sample-containing fluid may be introduced into the flow path via the inlet. Volumes of the sample-containing fluid may be isolated from one another on the flow path. A two-dimensional monolayer of the volumes may be imaged. The two-dimensional monolayer may be formed along the flow path between the inlet and the outlet.
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公开(公告)号：US10512910B2
公开(公告)日：2019-12-24
申请号：US15707908
申请日：2017-09-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Billy W. Colston, Jr. , Benjamin J. Hindson , Kevin D. Ness , Donald A. Masquelier
IPC: B01L3/00 , C12Q1/68 , C12Q1/686 , G01N35/08 , B01F13/00 , B01F3/08 , B01L9/00 , B01F13/10 , B01F5/00 , B01F5/02
Abstract: Droplet-based methods of analysis. In an exemplary method, a device having a port connected to a chamber may be selected. A sample-containing fluid may be placed into the port. A pressure differential may be created that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets. A two-dimensional monolayer of the droplets may be formed in the chamber. At least a portion of the monolayer may be imaged.
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