公开(公告)号：US12090480B2

公开(公告)日：2024-09-17

申请号：US18362530

申请日：2023-07-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, Jr.

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  B01F25/00 ,  B01F25/23 ,  B01F33/302 ,  G01N35/08

CPC classification number: B01L3/50273 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/813 ,  B01L3/502 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  C12Q1/686 ,  B01F25/14 ,  B01F25/23 ,  B01F33/3021 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  G01N35/08

Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.

公开(公告)号：US11499181B2

公开(公告)日：2022-11-15

申请号：US16235996

申请日：2018-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/6858 ,  C12Q1/683 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686

Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.

公开(公告)号：US10512910B2

公开(公告)日：2019-12-24

申请号：US15707908

申请日：2017-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Billy W. Colston, Jr. ,  Benjamin J. Hindson ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: B01L3/00 ,  C12Q1/68 ,  C12Q1/686 ,  G01N35/08 ,  B01F13/00 ,  B01F3/08 ,  B01L9/00 ,  B01F13/10 ,  B01F5/00 ,  B01F5/02

Abstract: Droplet-based methods of analysis. In an exemplary method, a device having a port connected to a chamber may be selected. A sample-containing fluid may be placed into the port. A pressure differential may be created that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets. A two-dimensional monolayer of the droplets may be formed in the chamber. At least a portion of the monolayer may be imaged.

公开(公告)号：US10258988B2

公开(公告)日：2019-04-16

申请号：US15365883

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B29L31/00

Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

公开(公告)号：US10167509B2

公开(公告)日：2019-01-01

申请号：US14848311

申请日：2015-09-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

公开(公告)号：US10166522B2

公开(公告)日：2019-01-01

申请号：US14938470

申请日：2015-11-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: C12Q1/68 ,  B01J19/00 ,  G01N21/75 ,  C12Q1/6844 ,  G01N33/50 ,  B01F13/00 ,  B01L3/00 ,  G01N35/08 ,  B01L7/00 ,  G01N1/30 ,  G01N1/44 ,  C07H21/02 ,  C40B50/08 ,  C40B60/14

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

公开(公告)号：US11612892B2

公开(公告)日：2023-03-28

申请号：US17110095

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Donald A. Masquelier ,  Billy W. Colston, Jr. ,  Benjamin J. Hindson

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  C12Q1/686 ,  G01N21/64 ,  B29C45/00 ,  G01N21/49 ,  B01F101/23 ,  B29L31/00

Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.

公开(公告)号：US11130134B2

公开(公告)日：2021-09-28

申请号：US16404659

申请日：2019-05-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Billy W. Colston, Jr. ,  Benjamin J. Hindson ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F3/08 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B29L31/00

Abstract: Method of performing a droplet-based assay. The method may include obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer, performing a reaction in the droplets while packed closely together in the monolayer; and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.

公开(公告)号：US20160076099A1

公开(公告)日：2016-03-17

申请号：US14848311

申请日：2015-09-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2537/16 ,  C12Q2563/159 ,  C12Q2537/165

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以使得样品中核酸的拷贝数估计可以进行。 本文还提供了用于确定样品中靶核酸的两个或多个拷贝（例如两个或多个拷贝是在同一染色体上还是在不同染色体上）或用于定相等位基因的连接的方法，组合物和试剂盒。

公开(公告)号：US11633739B2

公开(公告)日：2023-04-25

申请号：US17137274

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Donald A. Masquelier ,  Billy W. Colston, Jr. ,  Benjamin J. Hindson

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  C12Q1/686 ,  G01N21/64 ,  B29C45/00 ,  G01N21/49 ,  B01F101/23 ,  B29L31/00

Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.