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公开(公告)号：US12090480B2
公开(公告)日：2024-09-17
申请号：US18362530
申请日：2023-07-31
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC分类号： B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
摘要： Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US11499181B2
公开(公告)日：2022-11-15
申请号：US16235996
申请日：2018-12-28
发明人： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC分类号： C12Q1/6858 , C12Q1/683 , C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/686
摘要： Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US20190241947A1
公开(公告)日：2019-08-08
申请号：US16235996
申请日：2018-12-28
发明人： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, JR.
IPC分类号： C12Q1/6858 , C12Q1/6806 , C12Q1/686 , C12Q1/6827
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/683 , C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q2535/125 , C12Q2600/158 , C12Q2600/172 , C12Q2537/16 , C12Q2563/159 , C12Q2537/165
摘要： Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US10272432B2
公开(公告)日：2019-04-30
申请号：US16160783
申请日：2018-10-15
IPC分类号： B01L3/02 , B01L3/00 , B01F3/08 , B01F13/00 , C12Q1/6806 , G01N35/08 , G01N35/10 , G01N35/00 , B01L7/00
摘要： A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
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公开(公告)号：US20170151558A1
公开(公告)日：2017-06-01
申请号：US15351354
申请日：2016-11-14
发明人： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC分类号： B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
摘要： System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.
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公开(公告)号：US09649635B2
公开(公告)日：2017-05-16
申请号：US15351331
申请日：2016-11-14
CPC分类号： B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
摘要： System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well.
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公开(公告)号：US09636682B2
公开(公告)日：2017-05-02
申请号：US15351335
申请日：2016-11-14
CPC分类号： B01L9/527 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/0241 , B01L3/502 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L7/525 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/0684 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/088 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1006 , G01N2015/1415
摘要： System, including methods, apparatus, and kits, for forming emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well. The device also may include a channel network having a first channel, a second channel, and a third channel that meet one another in a droplet-generation region. The system also may comprise a holder for the device. The system further may comprise an instrument configured to operatively receive an assembly including the device and the holder and to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel.
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公开(公告)号：US20150307919A1
公开(公告)日：2015-10-29
申请号：US14791111
申请日：2015-07-02
CPC分类号： B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , Y02A90/26
摘要： Method of performing a multiplexed digital assay. The method may include (1) partitioning a sample containing at least two distinct targets into droplets; (2) amplifying the at least two distinct targets within the droplets; (3) detecting light from the droplets indicative of the presence or absence of each distinct target in each droplet; and (4) calculating a level of each distinct target based on the detected light.
摘要翻译： 执行复用数字测定的方法。 该方法可以包括（1）将含有至少两个不同目标的样品分成液滴; （2）在液滴内放大至少两个不同的目标; （3）检测来自液滴的光，指示每个液滴中每个不同目标的存在或不存在; 和（4）基于检测到的光来计算每个不同目标的水平。
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公开(公告)号：US12097495B2
公开(公告)日：2024-09-24
申请号：US17750195
申请日：2022-05-20
发明人： Benjamin J. Hindson , Serge Saxonov , Phillip Belgrader , Kevin D. Ness , Michael Y. Lucero , Billy W. Colston, Jr. , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC分类号： B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
摘要： The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
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公开(公告)号：US10099219B2
公开(公告)日：2018-10-16
申请号：US15357840
申请日：2016-11-21
摘要： A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
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