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公开(公告)号：US09212203B2
公开(公告)日：2015-12-15
申请号：US14598719
申请日：2015-01-16
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Larry J. Cummings , Mark A. Snyder
IPC: C07K1/14 , G01N33/538 , B01D15/38 , B01D15/42 , B01J20/04 , B01J20/281 , B01J20/282 , C07K16/00
CPC classification number: C07K1/14 , B01D15/3847 , B01D15/424 , B01J20/048 , B01J20/281 , B01J20/282 , C07K16/00
Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
Abstract translation: 本发明公开了中和磷灰石表面的方法，例如在色谱法和蛋白质洗脱之前。
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公开(公告)号：US10099157B2
公开(公告)日：2018-10-16
申请号：US14747221
申请日：2015-06-23
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Mark A. Snyder , Kimberly C. Brisack
IPC: G01N30/50 , B01J20/281 , B01D15/20 , B01D15/36 , C01B25/32 , C01B25/455 , G01N30/26 , B01D15/38 , B01D15/42 , B01J20/04 , B01J20/30 , B01J20/34
Abstract: Disclosed are compositions and methods for regenerating or reducing the deterioration of an apatite solid surface during, or subsequent to, a chromatographic procedure for purifying a target molecule from a sample, by treating the apatite solid surface with a buffered calcium solution, followed by a phosphate buffered solution, followed by an alkaline hydroxide. The buffered calcium solution, phosphate buffered solution, and alkaline hydroxide can be applied subsequent to a bind and elute or flow through purification procedure. The methods provide an increase in resin mass and/or particle strength compared to prior methods.
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公开(公告)号：US20150367252A1
公开(公告)日：2015-12-24
申请号：US14747221
申请日：2015-06-23
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Mark A. Snyder , Kimberly C. Brisack
CPC classification number: B01D15/203 , B01D15/361 , B01D15/3847 , B01J20/048 , B01J20/282 , B01J20/3085 , B01J20/34 , B01J20/3433 , B01J20/3475 , C01B25/32 , C01B25/327 , C01B25/455 , G01N30/26 , G01N30/48 , G01N30/50
Abstract: Disclosed herein are methods of regenerating apatite surfaces, for example after purification of a target analyte.
Abstract translation: 本文公开的是再生磷灰石表面的方法，例如在纯化目标分析物之后。
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公开(公告)号：US20150191505A1
公开(公告)日：2015-07-09
申请号：US14598719
申请日：2015-01-16
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Larry J. Cummings , Mark A. Snyder
CPC classification number: C07K1/14 , B01D15/3847 , B01D15/424 , B01J20/048 , B01J20/281 , B01J20/282 , C07K16/00
Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
Abstract translation: 本发明公开了中和磷灰石表面的方法，例如在色谱法和蛋白质洗脱之前。
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公开(公告)号：US10676502B2
公开(公告)日：2020-06-09
申请号：US15879239
申请日：2018-01-24
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Larry J. Cummings , Mark A. Snyder
IPC: C07K16/00 , B01J20/04 , B01J20/281 , B01J20/282 , C07K1/14 , B01D15/42 , B01D15/38 , B01D15/34
Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
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公开(公告)号：US11841350B2
公开(公告)日：2023-12-12
申请号：US17695672
申请日：2022-03-15
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Christopher Belisle , Mark A. Snyder
CPC classification number: G01N30/52 , B01D15/22 , G01N30/6021 , G01N2030/522
Abstract: A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.
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公开(公告)号：US20180155390A1
公开(公告)日：2018-06-07
申请号：US15879239
申请日：2018-01-24
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Larry J. Cummings , Mark A. Snyder
IPC: C07K1/14 , C07K16/00 , B01J20/282 , B01D15/38 , B01J20/04 , B01D15/42 , B01J20/281
CPC classification number: C07K1/14 , B01D15/3847 , B01D15/424 , B01J20/048 , B01J20/281 , B01J20/282 , C07K16/00
Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
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公开(公告)号：US09914749B2
公开(公告)日：2018-03-13
申请号：US14932080
申请日：2015-11-04
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Larry J. Cummings , Mark A. Snyder
IPC: C07K1/14 , C07K16/00 , B01D15/38 , B01D15/42 , B01J20/04 , B01J20/281 , B01J20/282
CPC classification number: C07K1/14 , B01D15/3847 , B01D15/424 , B01J20/048 , B01J20/281 , B01J20/282 , C07K16/00
Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
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公开(公告)号：US20160052961A1
公开(公告)日：2016-02-25
申请号：US14932080
申请日：2015-11-04
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Larry J. Cummings , Mark A. Snyder
CPC classification number: C07K1/14 , B01D15/3847 , B01D15/424 , B01J20/048 , B01J20/281 , B01J20/282 , C07K16/00
Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
