Detection Methods for Target DNA
    3.
    发明申请
    Detection Methods for Target DNA 审中-公开
    靶DNA的检测方法

    公开(公告)号:US20150140554A1

    公开(公告)日:2015-05-21

    申请号:US13983683

    申请日:2012-02-01

    IPC分类号: C12Q1/68

    摘要: The invention pertains to a safe, quick and reliable method of detecting the presence of a target DNA sequence in a sample. The invention also pertains to a system for detecting presence of a target DNA sequence in a biological sample. The system includes an ITC template that includes a probe binding sequence that binds to a corresponding ITC probe and a first and second flanking primer recognizing sequence that binds to a first and second primer, respectively. The system also includes an ITC probe that binds to the ITC template probe binding sequence. The ITC probe includes a first marker molecule. The system also includes a first primer that binds to the first flanking primer sequence and a second primer that binds to said second primer sequence. The system further includes a target DNA sequence probe, wherein the target DNA sequence probe comprises a second marker molecule.

    摘要翻译: 本发明涉及一种检测样品中目标DNA序列的存在的安全,快速和可靠的方法。 本发明还涉及用于检测生物样品中目标DNA序列的存在的系统。 该系统包括ITC模板,其包括结合相应的ITC探针的探针结合序列和分别与第一和第二引物结合的第一和第二侧翼引物识别序列。 该系统还包括结合ITC模板探针结合序列的ITC探针。 ITC探针包括第一标记分子。 该系统还包括结合第一侧翼引物序列的第一引物和结合所述第二引物序列的第二引物。 该系统还包括靶DNA序列探针,其中靶DNA序列探针包含第二标记分子。

    Production of recombinant AAV virions
    4.
    发明授权
    Production of recombinant AAV virions 有权
    重组AAV病毒粒子的生产

    公开(公告)号:US07927585B2

    公开(公告)日:2011-04-19

    申请号:US12020395

    申请日:2008-01-25

    申请人: Richard O. Snyder

    发明人: Richard O. Snyder

    摘要: Stocks of infectious rAAV are generated using yeast strains, bacterial strains, and bacteriophages engineered to express the required AAV proteins and harboring rAAV vector sequences. Stocks of rAAV virions of all serotypes and pseudotypes can be generated in prokaryotic and eukaryotic cells using the methods described herein.

    摘要翻译: 使用酵母菌株,细菌菌株和噬菌体产生感染性rAAV的库存,其工程化以表达所需的AAV蛋白质并含有rAAV载体序列。 所有血清型和假型的rAAV病毒粒子的库存可以使用本文所述的方法在原核和真核细胞中产生。