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公开(公告)号：US08288121B2
公开(公告)日：2012-10-16
申请号：US12165020
申请日：2008-06-30
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译： 本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。 组合物是微乳液，其可以包括水/油/表面活性剂微乳液，以及任选的辅助表面活性剂。 微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。
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公开(公告)号：US08512978B2
公开(公告)日：2013-08-20
申请号：US13644655
申请日：2012-10-04
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译： 本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。 组合物是微乳液，其可以包括水/油/表面活性剂微乳液，以及任选的辅助表面活性剂。 微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。
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公开(公告)号：US20130029376A1
公开(公告)日：2013-01-31
申请号：US13644655
申请日：2012-10-04
IPC分类号： G01N1/28
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译： 本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。组合物是微乳液，其可包括水/油/表面活性剂微乳液，以及任选的辅助表面活性剂。 微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。
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公开(公告)号：US20080261266A1
公开(公告)日：2008-10-23
申请号：US12165020
申请日：2008-06-30
IPC分类号： G01N1/28
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译： 本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。 组合物是微乳液，其可以包括水/油/表面活性剂微乳液，以及任选的助表面活性剂。 微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。
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5.发明申请LOW VOLATILITY HIGH TEMPERATURE TISSUE CONDITIONING CROSS-REFERENCE TO RELATED APPLICATION 审中-公开低挥发性高温组织调理相关申请的交叉参考公开(公告)号：US20090104654A1
公开(公告)日：2009-04-23
申请号：US11720705
申请日：2005-12-14
摘要： Solutions exhibiting little or no evaporative loss at elevated temperatures, i.e., in excess of 100° C., are employed in place of conventional aqueous-based antigen retrieval solutions.
摘要翻译： 使用在高温下（即超过100℃）几乎没有或没有蒸发损失的溶液代替常规的水性抗原检索溶液。
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公开(公告)号：US20090170152A1
公开(公告)日：2009-07-02
申请号：US12344564
申请日：2008-12-28
申请人： Ryan Reeser , Brian H. Kram , Vincent R. Rizzo , David Chafin , Jerome W. Kosmeder , Christopher Bieniarz
发明人： Ryan Reeser , Brian H. Kram , Vincent R. Rizzo , David Chafin , Jerome W. Kosmeder , Christopher Bieniarz
IPC分类号： G01N1/28
摘要： Solutions exhibiting little or no evaporative loss at elevated temperatures, i.e., in excess of 100° C., are employed in place of conventional aqueous-based antigen retrieval solutions.
摘要翻译： 使用在高温下（即超过100℃）几乎没有或没有蒸发损失的溶液代替常规的水性抗原检索溶液。
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7.发明授权Haptens, hapten conjugates, compositions thereof and method for their preparation and use 有权半抗原，半抗原共轭物，其组合物及其制备和使用方法公开(公告)号：US08846320B2
公开(公告)日：2014-09-30
申请号：US12660017
申请日：2010-02-17
申请人： Jerome W. Kosmeder , Mark Lefever , Donald Johnson , Michael Farrell , Zhanna Zhilina , Christopher Bieniarz
发明人： Jerome W. Kosmeder , Mark Lefever , Donald Johnson , Michael Farrell , Zhanna Zhilina , Christopher Bieniarz
IPC分类号： G01N33/53 , G01N33/532 , G01N33/58
CPC分类号： G01N33/5308 , C07D495/04 , C12Q1/6804 , C12Q1/6841 , C12Q1/6886 , G01N33/532 , G01N33/533 , G01N33/58 , G01N33/582 , G01N33/583 , Y10S435/961 , Y10S530/807
摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.
摘要翻译： 描述了用于执行多路诊断测定的方法，例如样品中两种或更多种不同靶标。 一个实施方案包括使样品与特异性结合两个或更多个不同靶标的两个或多个特异性结合部分接触。 两个或更多个特异性结合部分与不同的半抗原结合，至少一个半抗原是恶唑，吡唑，噻唑，除二硝基苯基以外的硝基芳基化合物，苯并呋咱，三萜烯，脲，硫脲， 旋叶，香豆素，环链烷醇，杂二芳基，偶氮芳基或苯并二氮杂。 将样品与两种或更多种可分别检测的不同的抗半抗原抗体接触。 两种或更多种不同的抗半抗原抗体可以缀合到不同的可检测标记上。
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8.发明申请SUBSTRATES FOR CHROMOGENIC DETECTION AND METHODS OF USE IN DETECTION ASSAYS AND KITS 有权用于染色体检测的基质和检测方法和试剂盒公开(公告)号：US20130115593A1
公开(公告)日：2013-05-09
申请号：US13809354
申请日：2011-08-12
CPC分类号： C07F9/65031 , G01N21/78
摘要： Embodiments of substrates and processes for chromogenic detection, and in particular pyrazolyl dihydrogen phosphate compounds, are disclosed.
摘要翻译： 公开了用于显色检测的底物和方法，特别是吡唑基二氢磷酸酯化合物的实施方案。
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公开(公告)号：US20130109019A1
公开(公告)日：2013-05-02
申请号：US13805978
申请日：2011-07-01
申请人： Adrian E. Murillo , Jerome W. Kosmeder , Eric May , William Day , Mark Lefever , Anne M. Pedata , Christopher Bieniarz , Phillip Miller
发明人： Adrian E. Murillo , Jerome W. Kosmeder , Eric May , William Day , Mark Lefever , Anne M. Pedata , Christopher Bieniarz , Phillip Miller
IPC分类号： C07D277/54 , C07C237/20 , C07D271/12 , C07D233/90 , C07D243/00 , C07D241/52
CPC分类号： C07D277/54 , A61K47/51 , C07C235/34 , C07C237/20 , C07C311/37 , C07D233/90 , C07D241/52 , C07D243/00 , C07D271/12 , G01N33/581 , G01N2333/908
摘要： Embodiments of hapten conjugates including a hapten, an optional linker, and a peroxidase-activatable aryl moiety are disclosed. In some embodiments, the peroxidase-activatable aryl moiety is tyramine or a tyramine derivative. Embodiments of methods for making and using the hapten conjugates also are disclosed. In particular embodiments, the hapten conjugates are used in a signal amplification assay. In certain embodiments, the hapten is an oxazole, a pyrazole, a thiazole, a benzofurazan, a triterpene, a urea, a thiourea other than a rhodamine thiourea, a nitroaryl other than dinitrophenyl or trinitrophenyl, a rotenoid, a cyclolignan, a heterobiaryl, an azoaryl, a benzodiazepine, or 7-diethylamino-3-carboxycoumarin. The hapten is coupled to the peroxidase-activatable aryl moiety directly or indirectly via a linker. In certain embodiments, the hapten conjugates are used in multiplexed assays.
摘要翻译： 公开了包含半抗原，任选接头和过氧化物酶活化的芳基部分的半抗原共轭物的实施方案。 在一些实施方案中，过氧化物酶可活化的芳基部分是酪胺或酪胺衍生物。 还公开了制备和使用半抗原共轭物的方法的实施方案。 在具体实施方案中，半抗原共轭物用于信号放大测定。 在某些实施方案中，半抗原是恶唑，吡唑，噻唑，苯并呋咱，三萜，脲，除罗丹明硫脲以外的硫脲，除二硝基苯基或三硝基苯基以外的硝基芳基，旋环体，环链烷基，异二芳基， 偶氮二芳基，苯并二氮杂或7-二乙基氨基-3-羧基香豆素。 半抗原通过接头直接或间接地与过氧化物酶活化的芳基部分偶联。 在某些实施方案中，半抗原共轭物用于多重测定。
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10.发明申请Antibody-Nanoparticle Conjugates and Methods for Making and Using Such Conjugates 有权抗体纳米颗粒结合体及其制备和使用方法公开(公告)号：US20130034854A1
公开(公告)日：2013-02-07
申请号：US13640944
申请日：2011-04-27
申请人： Julia Ashworth-Sharpe , Chol Steven Yun , Zhanna Zhilina , Adrian E. Murillo , Donald D. Johnson , Michael Farrell , Jerome W. Kosmeder , Christopher Bieniarz
发明人： Julia Ashworth-Sharpe , Chol Steven Yun , Zhanna Zhilina , Adrian E. Murillo , Donald D. Johnson , Michael Farrell , Jerome W. Kosmeder , Christopher Bieniarz
IPC分类号： C07K17/14 , G01N33/566 , G01N33/573 , G01N33/553 , C12Q1/68 , B82Y5/00
CPC分类号： G01N33/54346 , C07F9/12 , C07F9/5004 , C07F9/5022 , C07F9/5045 , C07F9/5728 , C07F9/65515 , C07F9/65517 , C07F9/65522 , G01N33/531 , G01N33/553 , G01N33/587 , G01N33/6854 , G01N2333/916
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.
摘要翻译： 本文公开了抗体 - 纳米颗粒缀合物，其包括直接连接到抗体或其片段的两个或更多个纳米颗粒（例如金，钯，铂，银，铜，镍，钴，铱或其两种或多种的合金） 金属硫醇键。 制备本文公开的抗体 - 纳米颗粒缀合物的方法包括使芳基膦 - 纳米颗粒复合物与还原抗体反应以产生抗体 - 纳米颗粒缀合物。 本文还公开了用于检测样品中靶分子的方法，其包括使用抗体 - 纳米颗粒缀合物（例如本文所述的抗体 - 纳米颗粒缀合物）和利用本文公开的方法检测靶分子的试剂盒。
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