公开(公告)号：US20160326601A1

公开(公告)日：2016-11-10

申请号：US14913343

申请日：2013-10-29

申请人： CHINA AGRICULTURE UNIVERSITY

发明人： Xiaoxiang HU ,  Xiaorong GU ,  Ying GUO ,  Zheya SHENG ,  Yanqiang WANG ,  Hao QU ,  Ning LI

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6888 ,  C12Q2600/124 ,  C12Q2600/156

摘要： The present invention provides a method for detecting a chicken Beard trait. Copy number variations of three DNA fragments were found to be present in the chromosomes of bearded chicken number 27, in the physical locations 1702269-1721521 bp, 4470331-4503417 bp, 3578409-3592890 bp; 3 molecular markers were determined, and 3 pairs of primers were designed for said molecular markers; the method of PCR is used for testing the chicken genome to be tested for the described 3 molecular markers; if the three target fragments of the PCR amplification reaction are 3200 bp, 501 bp, and 411 bp, then the detection result is positive; otherwise, the result is negative.

摘要翻译： 本发明提供了一种用于检测鸡胡子性状的方法。 发现三个DNA片段的拷贝数变异存在于27号胡子鸡的染色体中，物理位置1702269-1721521bp，4470331-4503417bp，3578409-3592890bp; 确定3个分子标记，为所述分子标记设计3对引物; PCR方法用于测试所测3个分子标记的鸡基因组; 如果PCR扩增反应的三个靶片段为3200bp，501bp和411bp，则检测结果为阳性; 否则，结果为负数。