公开(公告)号：US20030212259A1

公开(公告)日：2003-11-13

申请号：US10400630

申请日：2003-03-28

申请人： CHISSO CORPORATION

发明人： Satoshi Inouye

IPC分类号： G01N033/53 ,  C07H021/04 ,  C07K014/435 ,  C12P021/02 ,  C12N005/06

CPC分类号： C07K14/43595

摘要： This invention is to provide a photoprotein which binds with a ligand specific for a substance to be detected at a binding ratio of 1:1 such that the luminescence activity is not reduced by binding with the ligand, a conjugate comprising the luminescent photoprotein and ligand, and a substance detection method which employs the conjugate as a marker. A calcium-binding photoprotein is produced having cysteine residue introduced within the 4th amino acid residue from the amino-terminus. A conjugate is formed by binding a ligand specific for a substance to be detected to the calcium-binding photoprotein, in a binding ratio of 1:1, via the introduced cysteine residue. The conjugate may be utilized as a marker for a substance to be detected.

摘要翻译： 本发明提供一种光蛋白，其以与1:1配体比例的待测物质特异性的配体结合，使得通过与配体的结合不发生发光活性，包含发光光蛋白和配体的缀合物， 以及使用该共轭物作为标记物的物质检测方法。 产生钙结合光蛋白，其在氨基末端的第4个氨基酸残基内引入半胱氨酸残基。 通过引入的半胱氨酸残基以1：1的结合比例将待测物质特异性配体与钙结合光蛋白结合而形成缀合物。 缀合物可以用作待检测物质的标记物。

公开(公告)号：US20020102687A1

公开(公告)日：2002-08-01

申请号：US09842164

申请日：2001-04-26

申请人： CHISSO CORPORATION

发明人： Satoshi Inouye

IPC分类号： C12Q001/66 ,  C07H021/04 ,  C12N009/02

CPC分类号： C12N9/0069 ,  C07K16/40

摘要： The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.

摘要翻译： 本发明提供了编码Oplophorus萤光素酶的多核苷酸或多核苷酸，其由19kDa和35kDa蛋白质，或19kDa光蛋白，重组分泌性Oplophorus荧光素酶或由多核苷酸编码的19kDa光蛋白组成，含有 多核苷酸和用载体转化的宿主。 此外，本发明提供了生产重组Oplophorus萤光素酶或光蛋白的方法。 这些蛋白质可以通过使用重组表达载体培养宿主细胞或通过体外翻译系统重组产生。

公开(公告)号：US20040002127A1

公开(公告)日：2004-01-01

申请号：US10377714

申请日：2003-03-04

申请人： CHISSO CORPORATION

发明人： Satoshi Inouye

IPC分类号： C12Q001/66 ,  C07H021/04 ,  C12N009/06 ,  C12P021/02 ,  C12N005/06

CPC分类号： C12N9/0069 ,  C07K16/40

摘要： The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.

摘要翻译： 本发明提供了编码Oplophorus萤光素酶的多核苷酸或多核苷酸，其由19kDa和35kDa蛋白质，或19kDa光蛋白，重组分泌性Oplophorus荧光素酶或由多核苷酸编码的19kDa光蛋白组成，含有 多核苷酸和用载体转化的宿主。 此外，本发明提供了生产重组Oplophorus萤光素酶或光蛋白的方法。 这些蛋白质可以通过使用重组表达载体培养宿主细胞或通过体外翻译系统重组产生。