摘要:
An immersion set for retrofitting an immersion objective comprises at least one immersion fluid tank, at least one pump fluidly connected to the immersion fluid tank, and a control electronics unit configured at least to control the pump. A fastening device is configured to realize the load-bearing fastening of the at least one immersion fluid tank, the at least one pump and the control electronics unit on the immersion objective or on a mounting adapter for the immersion objective.
摘要:
A method for wavelength-selective and high spatial resolving fluorescence microscopy. In a specimen fluorescence emitters are repeatedly excited and specimen frames are produced with a microscope. The fluorescence emitters are excited to emit fluorescence radiation such that at least a sub-set is isolated in each frame and the positions of the isolated fluorescence emitters are localized with a localization precision exceeding the optical resolution and a high-resolution complete image is produced. The imaging beam path of the microscope has a spectrally selective element which, during production of the frames, generates a spectrally-dependent rotational asymmetric point-spread function, such that images of isolated fluorescence emitters have a rotational asymmetry which depends on a wavelength at which the isolated fluorescence emitters fluoresce, and the images of the isolated fluorescence emitters are analysed with respect to rotational asymmetry and an indication of the wavelength of the isolated fluorescence emitters is derived therefrom.
摘要:
An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane and the illumination is implemented in the plane. A detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective has an illumination correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives being arranged both in the illumination beam path and in the detection beam path.
摘要:
The present invention relates to a digital microscope that includes an objective lens for enlarged optical imaging of a sample in an image plane. An image with an optical resolution may be represented in the image plane by means of the objective lens. The microscope also includes an image sensor for converting the image, depicted on the image sensor by the objective lens, into an electrical signal. The image sensor includes a matrix of pixels by means of which a maximum image resolution of the image sensor is determined, which is finer than the optical resolution of the objective lens. The objective lens has a maximum magnification factor of at most 40. The optical resolution of the objective lens is defined as a minimum distance between two structures that are distinguishable in the image. The maximum image resolution of the image sensor is defined by a pixel pitch. A quotient of the minimum distance between two structures that are distinguishable in the image and the pixel pitch defines a scanning factor that is at least 5.
摘要:
A microscope, in particular according to any of the preceding claims, consisting of an illuminating device, comprising an illumination light source and an illumination beam path for illuminating the specimen with a light sheet, a detection device for detecting light emitted by the specimen, and an imaging optical unit, which images the specimen via an imaging objective in an imaging beam path at least partly onto the detection device, wherein the light sheet is essentially planar at the focus of the imaging objective or in a defined plane in proximity of the geometrical focus of the imaging objective, and wherein the imaging objective has an optical axis, which intersects the plane of the light sheet at an angle that is different from zero, preferably perpendicularly, wherein an amplitude and/or phase filter is provided in the illumination beam path, said filter acting as a sine spatial filter in that it limits the illumination light in at least one spatial direction by filtering the spatial frequencies that occur with a sine function and/or in that the illumination light is limited with regard to the phase and amplitude thereof in at least one spatial direction by a sine filter function, and/or a combined amplitude and phase filter is provided in the illumination beam path, said filter shaping the light sheet by influencing the transmission profile of the illumination light distribution with a sine filter function.
摘要:
A device and a method for imaging a sample arranged in an object plane. The device includes an optical relay system that images an area of the sample from the object plane into an intermediate image plane. The device may also include an optical imaging system with an objective having an optical axis that lies perpendicularly on the intermediate image plan, and which is focused on the intermediate image plane, with the result that the object plane can be imaged undistorted onto a detector. The device also can include an illumination apparatus for illuminating the sample with a light sheet, wherein the light sheet lies essentially in the object plane and defines an illumination direction, and wherein the normal of the object plane defines a detection direction.
摘要:
An apparatus for imaging a sample arranged in a first medium in an object plane. The apparatus includes an optical transmission system which images the sample in the object plane in an intermediate image in an intermediate image plane. The object plane and the intermediate image plane form an angle not equal to 90° with an optical axis of the transmission system. The apparatus further comprises an optical imaging system having an objective. The object plane may be imaged on a detector without distortion. The optical transmission system is symmetrical with respect to a pupil plane, the object plane, and the intermediate image plane to satisfy the Scheimpflug condition. The intermediate image lies in a second medium having a refractive index virtually identical to that of the first medium. A lens group of a subsystem arranged closest to the sample or intermediate image comprises at least one catadioptric assembly.
摘要:
A method for high-resolution 3D fluorescence microscopy, wherein fluorescence emitters in a sample are repeatedly excited to emit fluorescence, and still images are produced of the sample by means of a microscope. The microscope has an imaging beam path with an optical resolution and a focal plane, wherein the fluorescence emitters are excited to emit fluorescence in such a manner that at least a subset of the fluorescence emitters is isolated in each still image so that the images of these fluorescence emitters can be separated in the still images within the optical resolution. The positions of the fluorescence emitters are localized in the generated still images, from the images of the isolated fluorescence emitters, with a location accuracy exceeding the optical resolution, and a high-resolution composite image is generated therefrom.
摘要:
The invention relates to a device and a method for imaging a sample (2) arranged in an object plane (1). Such a device comprises an optical relay system (3) which images an area of the sample (2) from the object plane (1) into an intermediate image plane (4). Here, the object plane (1) and the intermediate image plane (4) with an optical axis (5) of the relay system (3) include an angle different from 90°. The optical relay system (3) is composed of several lenses. The device also comprises an optical imaging system (6) with an objective, the optical axis (7) of which lies perpendicularly on the intermediate image plane (4) and which is focused on the intermediate image plane (4), with the result that the object plane (1) can be imaged undistorted onto a detector (8). Finally, the device also comprises an illumination apparatus (10) for illuminating the sample (2) with a light sheet (11), wherein the light sheet (11) lies essentially in the object plane (1) and defines an illumination direction, and wherein the normal of the object plane (1) defines a detection direction.In such a device, the object plane (1) with the optical axis (5) of the relay system (3) includes an angle, the value of which is smaller than the aperture angle of an object-side detection aperture cone (12) of the relay system (3), and the object plane (1) lies at least partially within the object-side detection aperture cone (12). The intermediate image plane (4) with the optical axis (5) of the relay system (3) also includes an angle, the value of which is smaller than the aperture angle of an intermediate image-side detection aperture cone (13) of the relay system (3), and the intermediate image plane (4) lies at least partially within the intermediate image-side detection aperture cone (13).
摘要:
A microscope including a sample carrier configured to support a sample. Excitation light illuminates the sample via an excitation beam path. Detection light from the sample is guided to detection means via a detection beam path. Through an objective arranged along the optical axis, excitation light is guided in direction of the sample carrier and detection light coming from the sample is guided in direction of the detection means. Beam-splitting means separate excitation light and detection light. Also provided are means for generating a light sheet from excitation light, and means for illuminating the sample with this light sheet. The light sheet lies in a plane at a nonzero angle to the optical axis. The means for illuminating the sample include an optical-deflecting device arranged on or at the sample carrier, which deflects excitation light from the objective into the plane of the light sheet via an optically active surface.