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    COMPOSITIONS AND KITS FOR MOLECULAR COUNTING
    8.
    发明申请
    COMPOSITIONS AND KITS FOR MOLECULAR COUNTING 审中-公开
    分子计数的组合物和试剂盒

    公开(公告)号：US20150133319A1

    公开(公告)日：2015-05-14

    申请号：US14381488

    申请日：2013-02-27

    Applicant: CELLULAR RESEARCH, INC.

    Inventor： Glenn K. Fu ,  Stephen P.A. Fodor ,  Julie Wilhelmy

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1065 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6834 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

    Abstract: Methods, kits and systems are disclosed for analyzing one or more molecules in a sample. Analyzing the one or more molecules may comprise quantitation of the one or more molecules. Individual molecules may quantitated by PCR, arrays, beads, emulsions, droplets, or sequencing. Quantitation of individual molecules may further comprise stochastic labeling of the one or more molecules with a plurality of oligonucleotide tags to produce one or more stochastically labeled molecules. The methods may further comprise amplifying, sequencing, detecting, and/or quantifying the stochastically labeled molecules. The molecules may be DNA, RNA and/or proteins.

    Abstract translation: 公开了用于分析样品中的一种或多种分子的方法，试剂盒和系统。 分析一个或多个分子可以包括一个或多个分子的定量。 单个分子可以通过PCR，阵列，珠粒，乳液，液滴或测序来定量。 单个分子的定量可以进一步包括用多个寡核苷酸标签随机标记一个或多个分子以产生一个或多个随机标记的分子。 所述方法还可以包括放大，测序，检测和/或定量随机标记的分子。 分子可以是DNA，RNA和/或蛋白质。

    DIGITAL COUNTING OF INDIVIDUAL MOLECULES BY STOCHASTIC ATTACHMENT OF DIVERSE LABELS
    9.
    发明申请
    DIGITAL COUNTING OF INDIVIDUAL MOLECULES BY STOCHASTIC ATTACHMENT OF DIVERSE LABELS 有权
    个体分子数字数据通过多媒体标签的STOCHASTIC连接

    公开(公告)号：US20150099673A1

    公开(公告)日：2015-04-09

    申请号：US14540029

    申请日：2014-11-12

    Applicant: Cellular Research, Inc.

    Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

    IPC: C12N15/10 ,  G06F19/20

    CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

    Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

    Abstract translation: 公开了组合物，方法和试剂盒，用于通过附加不同标签组来随机标记相同分子的集合来进行高灵敏度单分子数字计数。 分子的每个拷贝从不同标签的非消耗性储库中随机选择。 检测可以通过多种方法，包括基于杂交或测序。 否则在信息内容中相同的分子可以被标记以产生在集合中是独特的或大致唯一的可单独检测的产物。 这种随机变换放宽了从定位和识别相同分子之一计数分子的问题到检测是否存在预编程标签的一系列二进制数字问题。 该方法可以用于例如估计样品中给定类型或类型的单独分子的数目。

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