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公开(公告)号：US10131958B1
公开(公告)日：2018-11-20
申请号：US16038887
申请日：2018-07-18
Applicant: Cellular Research, Inc.
Inventor： Christina Fan , Stephen P. A. Fodor , Glenn Fu , Geoffrey Richard Facer , Julie Wilhelmy
IPC: C12Q1/68 , C12Q1/6888 , C12Q1/6876 , C12Q1/6881 , C12Q1/6874
Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
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公开(公告)号：US09905005B2
公开(公告)日：2018-02-27
申请号：US15409355
申请日：2017-01-18
Applicant: Cellular Research, Inc.
Inventor： Glenn Fu , Roger Rudoff , David Stern , George T. Wu , Stephen P. A. Fodor , Geoffrey Facer
CPC classification number: G06T7/0012 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2201/129 , G06K9/6267 , G06T7/277 , G06T2207/10056 , G06T2207/10064 , G06T2207/30072 , G06T2207/30242
Abstract: Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.
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公开(公告)号：US09845502B2
公开(公告)日：2017-12-19
申请号：US14975441
申请日：2015-12-18
Applicant: Cellular Research, Inc.
Inventor： Stephen P. A. Fodor , Glenn K. Fu
CPC classification number: C12Q1/6874 , C12N15/1065 , C12Q1/6809 , C12Q1/6837 , C12Q1/6851 , C12Q1/686 , C12Q1/6869 , C12Q1/6876 , G06F19/20 , G06F19/22 , C12Q2565/102 , C12Q2537/157 , C12Q2525/191
Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
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公开(公告)号：US09727810B2
公开(公告)日：2017-08-08
申请号：US15055445
申请日：2016-02-26
Applicant: Cellular Research, Inc.
Inventor： Stephen P. A. Fodor , Christina Fan , Glenn Fu , Geoffrey Facer
CPC classification number: G06K19/06103 , C12Q1/6813 , C12Q1/6816 , C12Q2525/179 , C12Q2543/10 , C12Q2563/155
Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.
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公开(公告)号：US10927419B2
公开(公告)日：2021-02-23
申请号：US15987851
申请日：2018-05-23
Applicant: Cellular Research, Inc.
Inventor： Christina Fan , Stephen P. A. Fodor , Glenn Fu , Geoffrey Richard Facer , Julie Wilhelmy
IPC: C12Q1/68 , C12Q1/6888 , C12Q1/6874 , C12Q1/6876 , C12Q1/6881
Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
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Patent Agency Ranking
公开(公告)号：US09816137B2
公开(公告)日：2017-11-14
申请号：US14281706
申请日：2014-05-19
Applicant: Cellular Research, Inc.
Inventor： Stephen P. A. Fodor , Glenn K. Fu
CPC classification number: C12Q1/6874 , C12N15/1065 , C12Q1/6809 , C12Q1/6837 , C12Q1/6851 , C12Q1/686 , C12Q1/6869 , C12Q1/6876 , G06F19/20 , G06F19/22 , C12Q2565/102 , C12Q2537/157 , C12Q2525/191
Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
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公开(公告)号：US09598736B2
公开(公告)日：2017-03-21
申请号：US15167807
申请日：2016-05-27
Applicant: Cellular Research Inc.
Inventor： Christina Fan , Stephen P. A. Fodor , Glenn Fu , Geoffrey Richard Facer , Julie Wilhelmy
IPC: C12Q1/68
CPC classification number: C12Q1/6888 , C12Q1/6874 , C12Q1/6876 , C12Q1/6881 , C12Q2600/158 , C12Q2600/16 , C12Q2563/159 , C12Q2563/185
Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
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公开(公告)号：US09582877B2
公开(公告)日：2017-02-28
申请号：US14508911
申请日：2014-10-07
Applicant: Cellular Research, Inc.
Inventor： Glenn Fu , Roger Rudoff , David Stern , George T. Wu , Stephen P. A. Fodor , Geoffrey Facer
CPC classification number: G06T7/0012 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2201/129 , G06K9/6267 , G06T7/277 , G06T2207/10056 , G06T2207/10064 , G06T2207/30072 , G06T2207/30242
Abstract: Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.
Abstract translation: 描述了用于阵列的多个区域的数字成像的方法，系统和平台以及其上的标记特征的检测和计数。
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9.发明授权Digital counting of individual molecules by stochastic attachment of diverse labels 有权通过随机附着不同标签对个体分子的数字计数公开(公告)号：US09290809B2
公开(公告)日：2016-03-22
申请号：US14540018
申请日：2014-11-12
Applicant: Cellular Research, Inc.
Inventor： Stephen P. A. Fodor , Glenn K. Fu
CPC classification number: C12Q1/6874 , C12N15/1065 , C12Q1/6809 , C12Q1/6837 , C12Q1/6851 , C12Q1/686 , C12Q1/6869 , C12Q1/6876 , G06F19/20 , G06F19/22 , C12Q2565/102 , C12Q2537/157 , C12Q2525/191
Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
Abstract translation: 公开了组合物，方法和试剂盒，用于通过附加不同标签组来随机标记相同分子的集合来进行高灵敏度单分子数字计数。 分子的每个拷贝从不同标签的非消耗性储库中随机选择。 检测可以通过多种方法，包括基于杂交或测序。 否则在信息内容中相同的分子可以被标记以产生在集合中是独特的或大致唯一的可单独检测的产物。 这种随机变换放宽了从定位和识别相同分子之一计数分子的问题到检测是否存在预编程标签的一系列二进制数字问题。 该方法可以用于例如估计样品中给定类型或类型的单独分子的数目。
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公开(公告)号：US09290808B2
公开(公告)日：2016-03-22
申请号：US14540007
申请日：2014-11-12
Applicant: Cellular Research, Inc.
Inventor： Stephen P. A. Fodor , Glenn K. Fu
CPC classification number: C12Q1/6874 , C12N15/1065 , C12Q1/6809 , C12Q1/6837 , C12Q1/6851 , C12Q1/686 , C12Q1/6869 , C12Q1/6876 , G06F19/20 , G06F19/22 , C12Q2565/102 , C12Q2537/157 , C12Q2525/191
Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
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