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公开(公告)号:US20070243303A1
公开(公告)日:2007-10-18
申请号:US10562601
申请日:2004-07-02
申请人: Christiaan Dan Hengst , Olivera Gajic , Oscar Kuipers , Jan Kok , Jan Sikkema , Johannes Geurts , Arjen Nauta
发明人: Christiaan Dan Hengst , Olivera Gajic , Oscar Kuipers , Jan Kok , Jan Sikkema , Johannes Geurts , Arjen Nauta
CPC分类号: C12N15/746 , A23C9/12 , A23C19/032 , A23C2220/202
摘要: The invention relates to methods and means for regulating gene expression and to CodY target sequences. CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. Also, CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus are disclosed. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.
摘要翻译: 本发明涉及调节基因表达和CodY靶序列的方法和手段。 CodY通过与相应基因上游的特定DNA序列结合来抑制其靶基因。 通过分析在DNA微阵列研究中鉴定的δ编码乳酸乳球菌MG1363衍生物中的去甲基化基因的上游序列来鉴定保守的靶位点。 此外,公开了来自其它革兰氏阳性菌如枯草芽孢杆菌和链球菌的CodY靶序列。 因此,本发明提供可用于不同应用中的抑制或解除基因表达的CodY靶序列。