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1.发明授权Nucleotide sequence of the mycoplasma genitalium genome, fragments thereof, and uses thereof 失效支原体生殖器基因组的核苷酸序列，其片段及其用途公开(公告)号：US06537773B1
公开(公告)日：2003-03-25
申请号：US08545528
申请日：1995-10-19
申请人： Claire M. Fraser , Mark D. Adams , Jeannine D. Gocayne , Clyde A. Hutchison, III , Hamilton O. Smith , J. Craig Venter , Owen White
发明人： Claire M. Fraser , Mark D. Adams , Jeannine D. Gocayne , Clyde A. Hutchison, III , Hamilton O. Smith , J. Craig Venter , Owen White
IPC分类号： C12Q168
摘要： The present invention provides the nucleotide sequence of the entire genome of Mycoplasma genitalium, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies protein encoding fragments of the genome, and identifies, by position relative to two (2) genes known to flank the origin of replication, any regulatory elements which modulate the expression of the protein encoding fragments of the Mycoplasma genitalium genome.
摘要翻译： 本发明提供生殖生殖支原体（SEQ ID NO：1）的全基因组的核苷酸序列。 本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的蛋白质编码片段，并且通过相对于已知侧翼于复制起点的两个（2）基因的位置来鉴定调节编码蛋白质的蛋白质表达的任何调节元件 生殖支原体基因组的片段。
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2.发明授权Nucleotide sequence of the Haemophilus influenzae Rd genome, fragments thereof, and uses thereof 失效流感嗜血杆菌Rd基因组的核苷酸序列，其片段及其用途公开(公告)号：US06846651B2
公开(公告)日：2005-01-25
申请号：US10158865
申请日：2002-06-03
IPC分类号： C12N15/09 , A61K39/102 , A61P31/04 , A61P31/10 , A61P31/12 , C07K14/285 , C12N1/20 , C12N1/21 , C12N15/31 , C12N15/63
CPC分类号： C07K14/285 , Y10S435/851
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。 本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组
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3.发明授权公开(公告)号：US06528289B1
公开(公告)日：2003-03-04
申请号：US09643990
申请日：2000-08-23
IPC分类号： C12N1564
CPC分类号： C07K14/285
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
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公开(公告)号：US06468765B1
公开(公告)日：2002-10-22
申请号：US08487429
申请日：1995-06-07
IPC分类号： G12P2102
CPC分类号： C07K14/285
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。 本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组
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5.发明授权Nucleotide sequence of the Haemophilus influenzae Rd genome, fragments thereof, and uses thereof 失效流感嗜血杆菌Rd基因组的核苷酸序列，其片段及其用途公开(公告)号：US06506581B1
公开(公告)日：2003-01-14
申请号：US09557884
申请日：2000-04-25
IPC分类号： C12N121
CPC分类号： C07K14/285 , Y10S435/851
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。 本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组
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6.发明授权Computer readable genomic sequence of Haemophilus influenzae Rd, fragments thereof, and uses thereof 失效流感嗜血杆菌Rd的计算机可读基因组序列及其用途及其用途公开(公告)号：US06355450B1
公开(公告)日：2002-03-12
申请号：US08476102
申请日：1995-06-07
IPC分类号： C12P2106
CPC分类号： C07K14/285 , Y10S435/851
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO: 1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。本发明还提供存储在计算机可读介质上的序列信息以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组
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公开(公告)号：US09593329B2
公开(公告)日：2017-03-14
申请号：US12247126
申请日：2008-10-07
申请人： Daniel G. Gibson , Lei Young , John I. Glass , Gwynedd A. Benders , J. Craig Venter , Clyde A. Hutchison, III , Hamilton O. Smith
发明人： Daniel G. Gibson , Lei Young , John I. Glass , Gwynedd A. Benders , J. Craig Venter , Clyde A. Hutchison, III , Hamilton O. Smith
CPC分类号： C12N15/1093 , C12N15/81
摘要： A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.
摘要翻译： 描述了通过在体外组合盒来组装任何所需核酸分子以形成在体内进一步组合的组装或通过在酵母培养物中通过重组组装大量DNA片段以获得所需的相当大小的DNA分子来组装任何所需核酸分子的方法。
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公开(公告)号：US20110053272A1
公开(公告)日：2011-03-03
申请号：US12718911
申请日：2010-03-05
申请人： Gwynedd A. Benders , John I. Glass , Clyde A. Hutchison, III , Carole Lartigue , Sanjay Vashee , Mikkel A. Algire , Hamilton O. Smith , Charles E. Merryman , Vladimir N. Noskov , Ray-Yuan Chuang , Daniel G. Gibson , J. Craig Venter
发明人： Gwynedd A. Benders , John I. Glass , Clyde A. Hutchison, III , Carole Lartigue , Sanjay Vashee , Mikkel A. Algire , Hamilton O. Smith , Charles E. Merryman , Vladimir N. Noskov , Ray-Yuan Chuang , Daniel G. Gibson , J. Craig Venter
CPC分类号： C12N15/905 , C12N15/10 , C12N15/1031 , C12N15/1079 , C12N15/1093 , C12N15/81
摘要： Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
摘要翻译： 本文公开了用于在异源宿主细胞中克隆供体基因组的组合物和方法。 在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。 修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。 本文公开的方法可用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。
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公开(公告)号：US20090275086A1
公开(公告)日：2009-11-05
申请号：US12247126
申请日：2008-10-07
申请人： Daniel G. Gibson , Lei Young , John I. Glass , Gwynedd A. Benders , J. Craig Venter , Clyde A. Hutchison, III , Hamilton O. Smith
发明人： Daniel G. Gibson , Lei Young , John I. Glass , Gwynedd A. Benders , J. Craig Venter , Clyde A. Hutchison, III , Hamilton O. Smith
CPC分类号： C12N15/1093 , C12N15/81
摘要： A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.
摘要翻译： 描述了通过在体外组合盒来组装任何所需核酸分子以形成在体内进一步组合的组装或通过在酵母培养物中通过重组组装大量DNA片段以获得所需的相当大小的DNA分子来组装任何所需核酸分子的方法。
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公开(公告)号：US10041060B2
公开(公告)日：2018-08-07
申请号：US11635355
申请日：2006-12-06
摘要： Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.
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