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    Methods for detecting target nucleic acid sequences
    1.
    发明授权
    Methods for detecting target nucleic acid sequences 有权
    检测靶核酸序列的方法

    公开(公告)号：US06326145B1

    公开(公告)日：2001-12-04

    申请号：US09200232

    申请日：1998-11-25

    申请人： David Mark Whitcombe ,  Jane Theaker ,  Neil James Gibson ,  Stephen Little

    发明人： David Mark Whitcombe ,  Jane Theaker ,  Neil James Gibson ,  Stephen Little

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6853 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2531/113 ,  C12Q2525/186 ,  C12Q2525/161 ,  C12Q2565/1025 ,  C12Q2561/119 ,  C12Q2525/301

    摘要： A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerization thereof, under conditions such that the template binding region of the primer will hybridize to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product from the template whereupon the target binding region in the tail of the primer will hybridize to a sequence in the primer extension product corresponding to the target nucleic acid, and wherein any such target specific hybridization causes a detectable change in the signalling system, such that the presence or absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a detectable change in the signalling system.

    摘要翻译： 一种用于检测靶核酸的方法，所述方法包括使来自样品的模板核酸与（i）信号系统和（ii）具有模板结合区的尾部核酸引物接触，并且所述尾部包含接头和 在适当的核苷三磷酸和其聚合剂的存在下，在引物的模板结合区域将与模板核酸中的互补序列杂交并延伸以形成引物延伸产物的条件下， 将任何这样的产物与模板分离，因此引物尾部中的靶结合区域将与对应于靶核酸的引物延伸产物中的序列杂交，并且其中任何这样的靶特异性杂交引起信号系统中可检测的变化 ，使得通过参考前文检测样品中靶核酸的存在或不存在 信号系统中存在或不存在可检测的变化。

    Methods and primers for detecting target nucleic acid sequences
    2.
    发明授权
    Methods and primers for detecting target nucleic acid sequences 有权
    用于检测靶核酸序列的方法和引物

    公开(公告)号：US09273350B2

    公开(公告)日：2016-03-01

    申请号：US10862580

    申请日：2004-06-08

    申请人： David Mark Whitcombe ,  Jane Theaker ,  Neil James Gibson ,  Stephen Little

    发明人： David Mark Whitcombe ,  Jane Theaker ,  Neil James Gibson ,  Stephen Little

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6853 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2531/113 ,  C12Q2525/186 ,  C12Q2525/161 ,  C12Q2565/1025 ,  C12Q2561/119 ,  C12Q2525/301

    摘要： A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerisation thereof, under conditions such that the template binding region of the primer will hybridise to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product from the template whereupon the target binding region in the tail of the primer will hybridise to a sequence in the primer extension product corresponding to the target nucleic acid, and wherein any such target specific hybridisation causes a detectable change in the signalling system, such that the presence or absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a detectable change in the signalling system.

    摘要翻译： 一种用于检测靶核酸的方法，所述方法包括使来自样品的模板核酸与（i）信号系统和（ii）具有模板结合区的尾部核酸引物接触，并且所述尾部包含接头和 在适当的核苷三磷酸和其聚合剂的存在下，在引物的模板结合区域将与模板核酸中的互补序列杂交并延伸以形成引物延伸产物的条件下， 将任何这样的产物与模板分离，由此引物尾部中的靶结合区域将与对应于靶核酸的引物延伸产物中的序列杂交，并且其中任何这样的靶特异性杂交引起信号系统中可检测的变化 ，使得通过参考前文检测样品中靶核酸的存在或不存在 信号系统中存在或不存在可检测的变化。