公开(公告)号：US20050030601A1

公开(公告)日：2005-02-10

申请号：US10868590

申请日：2004-06-14

申请人： David Smith ,  Gregory Loney ,  Nathan Weiner ,  Christopher Miles ,  Albert Bukys ,  Akim Lennhoff ,  Robert Kuimelis

发明人： David Smith ,  Gregory Loney ,  Nathan Weiner ,  Christopher Miles ,  Albert Bukys ,  Akim Lennhoff ,  Robert Kuimelis

IPC分类号： G01N21/27 ,  G01N21/64 ,  G06F15/00

CPC分类号： G01N21/6452 ,  G01N21/278

摘要： In one embodiment a method for reducing variation in a plurality of scanners is described. The method comprises directing an excitation beam at a calibration standard in each of the plurality of scanners, where one of the plurality of scanners is a designated scanner; detecting emission data for each of the plurality of scanners from a plurality of fluorescent molecules disposed on the calibration standard, where the emission data is responsive to the excitation beam; determining variation in the emission data of one or more of the plurality of scanners based, at least in part, upon the emission data of the designated scanner; and adjusting one or more parameters in one or more of the plurality of scanners based, at least in part, upon the determined variation.

摘要翻译： 在一个实施例中，描述了用于减少多个扫描器中的变化的方法。 该方法包括以多个扫描仪中的每一个的校准标准来引导激励光束，其中多个扫描仪中的一个是指定的扫描器; 从布置在校准标准上的多个荧光分子检测多个扫描器中的每一个的发射数据，其中发射数据响应于激发光束; 至少部分地基于所指定的扫描仪的发射数据来确定所述多个扫描仪中的一个或多个的发射数据的变化; 以及至少部分地基于所确定的变化来调整所述多个扫描器中的一个或多个中的一个或多个参数。

公开(公告)号：US20050001176A1

公开(公告)日：2005-01-06

申请号：US10846261

申请日：2004-05-13

申请人： Gregory Loney ,  Nathan Weiner

发明人： Gregory Loney ,  Nathan Weiner

IPC分类号： B01J20060101 ,  G01N21/64 ,  G01N33/58

CPC分类号： G01N21/6452 ,  G01N21/6428 ,  G01N33/58 ,  G01N2021/6419 ,  G01N2021/6471

摘要： A method for scanning a probe array is described. The method comprises tuning an excitation light to a plurality of wavelengths each within an excitation range of a fluorescent label associated with one or more target molecules; and directing the tuned excitation light of each wavelength at the probe array.

摘要翻译： 描述了扫描探针阵列的方法。 该方法包括将激发光调谐到与一个或多个靶分子相关联的荧光标记的激发范围内的多个波长; 并将每个波长的调谐激发光引导到探针阵列。

公开(公告)号：US08797396B2

公开(公告)日：2014-08-05

申请号：US13312322

申请日：2011-12-06

申请人： Lou Dietz ,  Kurt Kuhlmann ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney

发明人： Lou Dietz ,  Kurt Kuhlmann ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney

IPC分类号： H04N7/18 ,  G02B21/36

CPC分类号： G02B21/365 ,  G02B21/0016 ,  G02B21/367 ,  H04N7/18

摘要： Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.

公开(公告)号：US20050142664A1

公开(公告)日：2005-06-30

申请号：US11017095

申请日：2004-12-20

申请人： Gregory Loney

发明人： Gregory Loney

IPC分类号： B01F11/02 ,  B01F13/00 ,  B01L3/00 ,  C40B60/14 ,  G01N33/543 ,  G01N35/00 ,  G01N1/00

CPC分类号： B01F11/0266 ,  B01F11/004 ,  B01F13/0059 ,  B01J2219/00484 ,  B01J2219/00486 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01L3/50273 ,  B01L2300/022 ,  B01L2300/0636 ,  B01L2400/0484 ,  C40B60/14 ,  G01N33/54373 ,  G01N2035/00158 ,  G01N2035/00524 ,  Y10T436/25

摘要： In one embodiment a method of mixing fluid is described that comprises providing a vibration comprising a resonant frequency; sympathetically amplifying the vibration in response to the resonant frequency; and modulating the resonant frequency, to cause the amplified vibration to generate turbulent flow in a fluid that influences the likelihood of interaction between a target molecule in the fluid with a probe on a biological probe array.

摘要翻译： 在一个实施例中，描述了混合流体的方法，其包括提供包括谐振频率的振动; 响应谐振频率同时放大振动; 并调制谐振频率，使放大的振动在流体中产生湍流，这影响流体中的目标分子与生物探针阵列上的探针之间的相互作用的可能性。

公开(公告)号：US20120092481A1

公开(公告)日：2012-04-19

申请号：US13312322

申请日：2011-12-06

申请人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

发明人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

IPC分类号： H04N7/18

CPC分类号： G02B21/365 ,  G02B21/0016 ,  G02B21/367 ,  H04N7/18

摘要： Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.

摘要翻译： 本文提供了系统方法，其包括用于数字病理应用的显微镜​​幻灯片扫描器的设计，其以低成本提供高质量图像和自动批量模式操作。 仪器架构有利地基于高性能但是低成本的计算技术，接口和软件标准的融合，以非常低的成本实现高质量数字显微镜。 还提供了一种部分地基于缝合方法的方法，其允许将图像划分成多个重叠的瓦片并且以放大率重新构建图像而不会显着损失精度。 扫描仪用于捕获快照图像。 该方法允许重叠在连续快照中拍摄的图像。

公开(公告)号：US20120076391A1

公开(公告)日：2012-03-29

申请号：US13312223

申请日：2011-12-06

申请人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

发明人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

IPC分类号： G06K9/46

CPC分类号： G02B21/365 ,  G02B21/0016 ,  G02B21/367 ,  H04N7/18

摘要： Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.

公开(公告)号：US20120076411A1

公开(公告)日：2012-03-29

申请号：US13312425

申请日：2011-12-06

申请人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

发明人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

IPC分类号： G06K9/34

CPC分类号： G02B21/365 ,  G02B21/0016 ,  G02B21/367 ,  H04N7/18

摘要： Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.

公开(公告)号：US20080240613A1

公开(公告)日：2008-10-02

申请号：US12054309

申请日：2008-03-24

申请人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

发明人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

IPC分类号： G06K9/36

CPC分类号： G02B21/365 ,  G02B21/0016 ,  G02B21/367 ,  H04N7/18

摘要： Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.

摘要翻译： 本文提供了系统方法，其包括用于数字病理应用的显微镜​​幻灯片扫描器的设计，其以低成本提供高质量图像和自动批量模式操作。 仪器架构有利地基于高性能但是低成本的计算技术，接口和软件标准的融合，以非常低的成本实现高质量数字显微镜。 还提供了一种部分地基于缝合方法的方法，其允许将图像划分成多个重叠的瓦片并且以放大率重新构建图像而不会显着损失精度。 扫描仪用于捕获快照图像。 该方法允许重叠在连续快照中拍摄的图像。

公开(公告)号：US20120076436A1

公开(公告)日：2012-03-29

申请号：US13313112

申请日：2011-12-07

申请人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

发明人： Lou Dietz ,  Kurt Kuhlmann ,  Lokesh Tatke ,  Suraj Somwanshi ,  Christopher Todd ,  Glenn Stark ,  Barry Rodriguez ,  Gregory Loney ,  Bikash Sabata ,  Ronald L. Allen ,  Suhas Patil ,  Sujit Chivate ,  Prashanth Ravindran ,  Sanford Barsky

IPC分类号： G06K9/36

CPC分类号： G02B21/365 ,  G02B21/0016 ,  G02B21/367 ,  H04N7/18

摘要： Provided herein are systems methods including a design of a microscope slide scanner for digital pathology applications which provides high quality images and automated batch-mode operation at low cost. The instrument architecture is advantageously based on a convergence of high performance, yet low cost, computing technologies, interfaces and software standards to enable high quality digital microscopy at very low cost. Also provided is a method based in part on a stitching method that allows for dividing an image into a number of overlapping tiles and reconstituting the image with a magnification without substantial loss of accuracy. A scanner is employed in capturing snapshot images. The method allows for overlapping images captured in consecutive snapshots.