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8 条记录 (耗时 0.047 秒)

    Cell-free assay for compounds which activate or block inactivation of cell proliferation
    5.
    发明授权
    Cell-free assay for compounds which activate or block inactivation of cell proliferation 失效
    激活或阻断细胞增殖失活的化合物的无细胞测定

    公开(公告)号：US5607833A

    公开(公告)日：1997-03-04

    申请号：US420339

    申请日：1995-04-11

    申请人： Michael Z. Gilman ,  Henry B. Sadowski

    发明人： Michael Z. Gilman ,  Henry B. Sadowski

    IPC分类号： G01N33/74 ,  C12Q1/68

    CPC分类号： G01N33/74 ,  G01N2500/00 ,  Y10S436/813

    摘要： This invention pertains to a method of assessing the ability of a compound to block or activate cell proliferation by assessing its effect on a cellular signal transduction pathway whose activation results in cell proliferation. In the present method, a cell-free system is used to assess the ability of a compound to block or activate a signal transduction pathway which is activated by binding of a growth factor to a cell surface receptor with intrinsic tyrosine kinase activity and whose activation results in proliferation of the cells to which growth factor has bound. Cellular signal transduction involves multiple pathways, each consisting of one or more steps emanating from a specific protein bound to a single receptor which is specific for that protein and located on the cell surface or within the cell.

    摘要翻译： 本发明涉及通过评估化合物阻断或激活细胞增殖的能力来评估其对细胞信号转导途径的影响的方法，其活化导致细胞增殖。 在本方法中，使用无细胞系统来评估化合物阻断或激活信号转导途径的能力，其通过将生长因子与细胞表面受体与内在酪氨酸激酶活性结合而激活，并且其激活结果 在生长因子结合的细胞的增殖中。 细胞信号转导涉及多个途径，每个途径包含一个或多个步骤，从与该蛋白质特异性并位于细胞表面或细胞内的单个受体结合的特异性蛋白质发出。

    Heterologous transcription factors
    8.
    发明授权
    Heterologous transcription factors 失效
    异源转录因子

    公开(公告)号：US06306649B1

    公开(公告)日：2001-10-23

    申请号：US08672213

    申请日：1996-06-27

    申请人： Michael Z. Gilman ,  Sridaran Natesan

    发明人： Michael Z. Gilman ,  Sridaran Natesan

    IPC分类号： C12N500

    CPC分类号： C07K14/4702 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16622

    摘要： This invention provides novel materials and methods involving the heterologous expression of transcription factors which are useful for effecting transcription of target genes in genetically engineered cells or organisms containing them. Target gene constructs and other materials useful for practicing the invention are also disclosed.

    摘要翻译： 本发明提供涉及转录因子的异源表达的新型材料和方法，其可用于在遗传工程化的细胞或含有它们的生物体中实现靶基因的转录。 还公开了靶基因构建体和其他可用于实施本发明的材料。