STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY
    2.
    发明申请
    STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 有权
    修改农杆菌菌株以增加植物转化频率

    公开(公告)号:US20150267213A1

    公开(公告)日:2015-09-24

    申请号:US13812469

    申请日:2011-07-29

    IPC分类号: C12N15/82

    摘要: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

    摘要翻译: 提供了在能够独立于农杆菌染色体,Ti质粒和植物转化双向载体复制的质粒上携带转化增强基因的土壤杆菌菌株,以及这些农杆菌菌株的用途。 另外,缺乏DNA重组功能的农杆菌菌株导致植物转化双向载体的不稳定或重排,并且能够独立于农杆菌染色体,Ti质粒和植物转化二元体的能够复制的质粒上转录增强基因 还提供了这些菌株的载体和用途。 还包括农杆菌菌株,其在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处将整合到农杆菌染色体中的转化增强基因并用于这些农杆菌菌株。 还描述了使用这些农杆菌菌株制备的植物。

    INSECT RESISTANCE MANAGEMENT WITH COMBINATIONS OF CRY1BE AND CRY1F PROTEINS
    5.
    发明申请
    INSECT RESISTANCE MANAGEMENT WITH COMBINATIONS OF CRY1BE AND CRY1F PROTEINS 有权
    CRY1BE和CRY1F蛋白质组合的耐药性管理

    公开(公告)号:US20120311746A1

    公开(公告)日:2012-12-06

    申请号:US13516629

    申请日:2010-12-16

    摘要: The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

    摘要翻译: 本发明部分涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。 如本文更详细讨论的那样,本发明的对象蛋白质是特别有利的组合,因为没有其他蛋白质对可以提供针对草地贪夜蛾(Spodoptera frugiperda)(FAW)和鸵鸟鸵鸟(Ostrinia nubilalis)的高水平的控制和非交叉抗性活性 ECB)昆虫。 这种双重非交叉抗性活性也是有利的,因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。 这可以减少或消除避难所面积的需要。 因此,本发明还通常涉及使用四个基因提供用于第一昆虫的非交叉耐药性控制的三种蛋白质和用于第二种昆虫的非交叉耐药性控制的三种蛋白质。 在优选的实施方式中,目标昆虫是FAW和ECB。