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    Methods of making active antibodies from biological fluids
    4.
    发明授权
    Methods of making active antibodies from biological fluids 有权

    公开(公告)号：US11067583B2

    公开(公告)日：2021-07-20

    申请号：US16302525

    申请日：2017-05-25

    申请人： University of Maryland, Baltimore ,  The United States of America as represented by the Department of Veterans Affairs

    发明人： Mohammad Sajadi ,  Anthony DeVico ,  George Lewis

    IPC分类号： C07K16/00 ,  C07H21/04 ,  C12P21/08 ,  A61K39/395 ,  C07K1/00 ,  G01N33/68 ,  C07K1/22 ,  C07K16/10 ,  H01J49/26

    摘要： The present invention provides a method of making an antibody by identifying a circulating antibody with activity from a subject comprising i) subjecting biological fluid selected from the group consisting of blood, plasma and serum and combinations thereof from the subject to one or more rounds of affinity chromatography to purify the circulating antibody; ii) optionally further subjecting the circulating antibody to isoelectric focusing to purify the circulating antibody based on charge; iii) testing the purified circulating antibody for activity; iv) digesting the purified circulating antibody from parts i) or ii) to create an antibody fragment; v) subjecting the antibody fragment to mass spectrometry to generate a mass assignment and a deduced amino acid sequence of the antibody fragment; vi) comparing the deduced amino acid sequence with an amino acid sequence of an antibody generated from the subject's B-cells to identify an antibody sequence that matches the deduced amino acid sequence; vii) generating an antibody comprising light chain and heavy chain CDR sequences of the B-cell antibody that matches the deducted amino acid sequence of party vi); and viii) testing the antibody of part vii) for activity.

    METHODS OF MAKING ACTIVE ANTIBODIES FROM BIOLOGICAL FLUIDS
    5.
    发明申请
    METHODS OF MAKING ACTIVE ANTIBODIES FROM BIOLOGICAL FLUIDS 审中-公开

    公开(公告)号：US20200182883A1

    公开(公告)日：2020-06-11

    申请号：US16302525

    申请日：2017-05-25

    申请人： University of Maryland, Baltimore ,  The United States of America as represented by the Department of Veterans Affairs

    发明人： Mohammad Sajadi ,  Anthony DeVico ,  George Lewis

    IPC分类号： G01N33/68 ,  C07K16/10 ,  C07K1/22

    摘要： The present invention provides a method of making an antibody by identifying a circulating antibody with activity from a subject comprising i) subjecting biological fluid selected from the group consisting of blood, plasma and serum and combinations thereof from the subject to one or more rounds of affinity chromatography to purify the circulating antibody; ii) optionally further subjecting the circulating antibody to isoelectric focusing to purify the circulating antibody based on charge; iii) testing the purified circulating antibody for activity; iv) digesting the purified circulating antibody from parts i) or ii) to create an antibody fragment; v) subjecting the antibody fragment to mass spectrometry to generate a mass assignment and a deduced amino acid sequence of the antibody fragment; vi) comparing the deduced amino acid sequence with an amino acid sequence of an antibody generated from the subject's B-cells to identify an antibody sequence that matches the deduced amino acid sequence; vii) generating an antibody comprising light chain and heavy chain CDR sequences of the B-cell antibody that matches the deducted amino acid sequence of party vi); and viii) testing the antibody of part vii) for activity.