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    Methods, compositions, and automated systems for separating rare cells from fluid samples
    5.
    发明授权
    Methods, compositions, and automated systems for separating rare cells from fluid samples 有权
    用于从流体样品中分离稀有细胞的方法，组合物和自动化系统

    公开(公告)号：US07166443B2

    公开(公告)日：2007-01-23

    申请号：US10701684

    申请日：2003-11-04

    申请人： George Walker ,  Junquan Xu ,  Douglas Yamanishi ,  Paul Hujsak ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Sara Snyder ,  Charina Yap

    发明人： George Walker ,  Junquan Xu ,  Douglas Yamanishi ,  Paul Hujsak ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Sara Snyder ,  Charina Yap

    IPC分类号： C12Q1/04

    CPC分类号： B01L3/502761 ,  A61M1/36 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3679 ,  B01D57/02 ,  B01D61/18 ,  B01D67/0034 ,  B01D67/0062 ,  B01D71/02 ,  B01D71/027 ,  B01D71/04 ,  B01D2325/021 ,  B01D2325/04 ,  B01L3/502 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0644 ,  B01L2400/065 ,  G01N1/40 ,  G01N15/0272 ,  G01N15/05 ,  G01N2001/4016 ,  G01N2015/0073 ,  G01N2015/0288 ,  G01N2015/0294 ,  G01N2015/0681

    摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells from a complex fluid sample. In particular, the enrichment of fetal cells from maternal samples, such as maternal blood samples, can greatly aid in the detection of fetal abnormalities or a variety of genetic conditions. In addition, the present invention recognizes that the enrichment of rare malignant cells from patient samples, can aid in diagnosis, prognosis, and development of therapeutic modalities for patients. The invention includes microfabricated filters for filtering fluid samples and methods of enriching rare cells of fluid samples using microfabricated filters of the present invention. The invention also includes solutions for the selective sedimentation of red blood cells (RBCs) from a blood sample and methods of using selective RBC sedimentation solutions for enriching rare cells of a fluid sample. Yet another aspect of the invention is an automated system for processing a fluid sample that includes: at least one filtration chamber that includes a microfabricated filter; automated means for directing fluid flow through at least one filtration chamber of the automated system, and means for collecting enriched rare cells. The present invention also includes methods of using automated systems for separating rare cells from fluid samples. Preferred fluid samples are blood, effusion, or urine samples, and rare cells that can be enriched from such sample include nucleated red blood cells and cancer cells.

    摘要翻译： 本发明认识到许多病症的诊断和预后可以取决于来自复杂流体样品的稀有细胞的富集。 特别地，母体样品（例如母体血液样品）富集胎儿细胞可以大大有助于检测胎儿异常或各种遗传病症。 此外，本发明认识到，来自患者样品的稀有恶性细胞的富集可以帮助患者的治疗方式的诊断，预后和发展。 本发明包括用于过滤液体样品的微制造过滤器和使用本发明的微加工过滤器来富集稀有细胞液体样品的方法。 本发明还包括从血液样品中选择性沉降红细胞（RBC）的方案和使用选择性RBC沉降溶液富集流体样品稀有细胞的方法。 本发明的另一方面是一种用于处理流体样品的自动化系统，该系统包括：至少一个包括微加工过滤器的过滤室; 用于引导流体流过自动化系统的至少一个过滤室的自动装置，以及用于收集富集的稀有细胞的装置。 本发明还包括使用自动化系统从流体样品中分离稀有细胞的方法。 优选的流体样品是血液，渗出物或尿液样品，并且可以从这种样品中富集的稀有细胞包括成核红细胞和癌细胞。

    High-density ion transport measurement biochip devices and methods
    7.
    发明申请
    High-density ion transport measurement biochip devices and methods 审中-公开
    高密度离子传输测量生物芯片器件及方法

    公开(公告)号：US20050196746A1

    公开(公告)日：2005-09-08

    申请号：US11033015

    申请日：2005-01-10

    申请人： Jia Xu ,  Antonio Guia ,  George Walker ,  Mingxian Huang ,  Lei Wu ,  Sithiphong Khachonesin ,  Julian Yuan

    发明人： Jia Xu ,  Antonio Guia ,  George Walker ,  Mingxian Huang ,  Lei Wu ,  Sithiphong Khachonesin ,  Julian Yuan

    IPC分类号： C12Q1/00 ,  G01N33/487

    CPC分类号： G01N33/48728

    摘要： The present invention provides novel biochips, biochip-based devices, and device configurations that can be used for ion transport measurement. The chips, devices, and designs of the present invention are particularly suited to high-throughput assays such as compound screening assays using patch clamping techniques. The invention includes high-density biochips made by novel methods and methods of making high density biochips, and also provides novel upper chamber configurations and fluidics designs for upper chambers of ion transport measurement devices that can be used in high throughput patch clamp assays. The present invention also includes methods of using ion transport measuring chips and devices of the present invention.

    摘要翻译： 本发明提供了可用于离子传输测量的新型生物芯片，基于生物芯片的器件和器件配置。 本发明的芯片，器件和设计特别适用于高通量测定，例如使用贴片钳技术的复合筛选测定法。 本发明包括通过制备高密度生物芯片的新方法和方法制备的高密度生物芯片，并且还提供了可用于高通量膜片钳测定的离子传输测量装置的上腔室的新型上室构型和流体学设计。 本发明还包括使用本发明的离子传输测量芯片和器件的方法。

    HIGH DENSITY INTEGRATED CIRCUIT APPARATUS, TEST PROBE AND METHODS OF USE THEREOF
    10.
    发明申请
    HIGH DENSITY INTEGRATED CIRCUIT APPARATUS, TEST PROBE AND METHODS OF USE THEREOF 审中-公开
    高密度集成电路设备，测试探针及其使用方法

    公开(公告)号：US20080106872A1

    公开(公告)日：2008-05-08

    申请号：US11930045

    申请日：2007-10-30

    申请人： Brian Beaman ,  Keith Fogel ,  Paul Lauro ,  Maurice Norcott ,  Da-Yuan Shih ,  George Walker

    发明人： Brian Beaman ,  Keith Fogel ,  Paul Lauro ,  Maurice Norcott ,  Da-Yuan Shih ,  George Walker

    IPC分类号： H05K1/14

    CPC分类号： G01R1/0735 ,  G01R1/06744 ,  G01R1/0675 ,  G01R1/07307 ,  G01R1/07378 ,  G01R3/00

    摘要： The present invention is directed to a high density test probe which provides a means for testing a high density and high performance integrated circuits in wafer form or as discrete chips. The test probe is formed from a dense array of elongated electrical conductors which are embedded in an compliant or high modulus elastomeric material. A standard packaging substrate, such as a ceramic integrated circuit chip packaging substrate is used to provide a space transformer Wires are bonded to an array of contact pads on the surface of the space transformer. The space transformer formed from a multilayer integrated circuit chip packaging substrate. The wires are as dense as the contact location array. A mold is disposed surrounding the array of outwardly projecting wires. A liquid elastomer is disposed in the mold to fill the spaces between the wires. The elastomer is cured and the mold is removed, leaving an array of wires disposed in the elastomer and in electrical contact with the space transformer The space transformer can have an array of pins which are on the opposite surface of the space transformer opposite to that on which the elongated conductors are bonded. The pins are inserted into a socket on a second space transformer, such as a printed circuit board to form a probe assembly. Alternatively, an interposer electrical connector can be disposed between the first and second space transformer.

    摘要翻译： 本发明涉及一种高密度测试探针，其提供用于测试晶片形式的高密度和高性能集成电路或作为离散芯片的装置。 测试探针由嵌入柔性或高模量弹性体材料的细长电导体的密集阵列形成。 陶瓷集成电路芯片封装基板等标准封装基板用于提供空间变压器引线与空间变压器表面的接触焊盘阵列接合。 空间变压器由多层集成电路芯片封装基板形成。 电线与接触位置阵列一样密集。 围绕着向外突出的线的阵列设置模具。 液体弹性体设置在模具中以填充电线之间的空间。 弹性体被固化并且模具被去除，留下布置在弹性体中并且与空间变压器电接触的线阵列。空间变压器可以具有一组引脚，这些引脚位于空间变压器的相反表面上， 其中细长导体被接合。 这些销插入第二空间变压器（例如印刷电路板）上的插座中以形成探针组件。 或者，插入器电连接器可以设置在第一和第二空间变压器之间。