公开(公告)号：US08084212B2

公开(公告)日：2011-12-27

申请号：US12887403

申请日：2010-09-21

申请人： Edgar Ong ,  Maurice Exner

发明人： Edgar Ong ,  Maurice Exner

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H19/34

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the IS1245 gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as IS1245 and DT1 genes and as probes as well as kits containing the oligonucleotides.

摘要翻译： 公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。 特别地，优选在扩增后检测鸟分枝杆菌的IS1245基因和胞内分枝杆菌的DT1基因。 此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。 还描述了可用作引物扩增靶基因如IS1245和DT1基因以及作为探针的寡核苷酸以及含有寡核苷酸的试剂盒。

公开(公告)号：US09797018B2

公开(公告)日：2017-10-24

申请号：US13296046

申请日：2011-11-14

申请人： Edgar Ong ,  Maurice Exner

发明人： Edgar Ong ,  Maurice Exner

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotide.

公开(公告)号：US20110027792A1

公开(公告)日：2011-02-03

申请号：US12887403

申请日：2010-09-21

申请人： Edgar Ong ,  Maurice Exner

发明人： Edgar Ong ,  Maurice Exner

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.

摘要翻译： 公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。 特别地，优选在扩增后检测鸟分枝杆菌的mig基因和胞内分枝杆菌的DT1基因。 此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。 还描述了可用作扩增靶基因如mig和DT1基因以及作为探针以及含有寡核苷酸的试剂盒的寡核苷酸。

公开(公告)号：US07824858B2

公开(公告)日：2010-11-02

申请号：US11338431

申请日：2006-01-23

申请人： Edgar Ong ,  Maurice Exner

发明人： Edgar Ong ,  Maurice Exner

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.

摘要翻译： 公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。 特别地，优选在扩增后检测鸟分枝杆菌的mig基因和胞内分枝杆菌的DT1基因。 此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。 还描述了可用作扩增靶基因如mig和DT1基因以及作为探针以及含有寡核苷酸的试剂盒的寡核苷酸。

公开(公告)号：US20120088242A1

公开(公告)日：2012-04-12

申请号：US13296046

申请日：2011-11-14

申请人： Edgar Ong ,  Maurice Exner

发明人： Edgar Ong ,  Maurice Exner

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotide

摘要翻译： 公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。 特别地，优选在扩增后检测鸟分枝杆菌的mig基因和胞内分枝杆菌的DT1基因。 此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。 还描述了可用作扩增靶基因如mig和DT1基因以及作为探针的寡核苷酸以及含有寡核苷酸的试剂盒

公开(公告)号：US20070172838A1

公开(公告)日：2007-07-26

申请号：US11338431

申请日：2006-01-23

申请人： Edgar Ong ,  Maurice Exner

发明人： Edgar Ong ,  Maurice Exner

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M avium and M intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.

摘要翻译： 公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。 特别地，优选在扩增后检测M avium的mig基因和胞内分枝杆菌的DT1基因。 此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。 还描述了可用作扩增靶基因如mig和DT1基因以及作为探针以及含有寡核苷酸的试剂盒的寡核苷酸。

公开(公告)号：US20070141559A1

公开(公告)日：2007-06-21

申请号：US11314202

申请日：2005-12-20

申请人： Maurice Exner

发明人： Maurice Exner

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/705

摘要： Detailed herein are methods of detecting herpes simplex virus and differentiating between types 1 and 2 by simultaneous detection of type-specific gene sequences. In particular aspects, individuals infected with HSV-1 can be distinguished from those infected with HSV-2 by amplification and detection of the HSV-1 glycoprotein B gene or the HSV-2 UL-8 gene. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided.

摘要翻译： 本文详细描述了通过同时检测类型特异性基因序列来检测单纯疱疹病毒并区分类型1和2之间的方法。 在特定方面，通过HSV-1糖蛋白B基因或HSV-2 UL-8基因的扩增和检测可以将感染HSV-1的个体与感染HSV-2的个体区分开。 提供用于HSV-1和HSV-2的差异检测的引物和探针。

公开(公告)号：US20140234830A1

公开(公告)日：2014-08-21

申请号：US14130705

申请日：2012-07-06

申请人： Maurice Exner ,  Luca Jacky ,  Yin-Peng Chen ,  Huong Mai ,  Michelle M. Tabb ,  Michael Aye

发明人： Maurice Exner ,  Luca Jacky ,  Yin-Peng Chen ,  Huong Mai ,  Michelle M. Tabb ,  Michael Aye

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/701 ,  C12Q1/68 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Q1/689 ,  C12Q1/70 ,  C12Q2527/101 ,  C12Q2527/125

摘要： Provided are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Further provided are reagent mixtures that allow for direct amplification of a sample, without the step of nucleic acid extraction.

摘要翻译： 提供的是使用直接扩增从微生物鉴定靶核酸的存在或不存在的方法，而不提取核酸的步骤，但是保留测定提取的核酸的方法的基本相同的特异性和灵敏度。 还提供允许直接扩增样品的试剂混合物，而不需要提取核酸步骤。

公开(公告)号：US08263330B1

公开(公告)日：2012-09-11

申请号：US11075825

申请日：2005-03-08

申请人： Maurice Exner

发明人： Maurice Exner

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

CPC分类号： C12Q1/689

摘要： Disclosed is a method for determining the presence of Mycobacterium tuberculosis complex nucleic acids in a test sample. In particular, regions of the IS6110 preferential locus (ipl) 3′-flanking region of the Mycobacterium tuberculosis complex genome are amplified and detected. In addition, oligonucleotides that can be used as primers to amplify the ipl 3′-flanking region and probe oligonucleotides are described.

摘要翻译： 公开了一种用于确定测试样品中结核分枝杆菌复合核酸的存在的方法。 特别地，扩增和检测结核分枝杆菌复合物基因组的IS6110优先位点（ipl）3'-侧翼区域。 此外，描述了可用作引物以扩增ipl 3'侧翼区和探针寡核苷酸的寡核苷酸。

公开(公告)号：US20100291583A1

公开(公告)日：2010-11-18

申请号：US12845465

申请日：2010-07-28

申请人： Maurice Exner ,  Amy Rogers

发明人： Maurice Exner ,  Amy Rogers

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q2563/173 ,  C12Q2527/107

摘要： Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.

摘要翻译： 公开了用于鉴定样品中核酸的方法。 在一个实例中，该方法包括：（a）使样品中的核酸与样品中核酸特异的寡核苷酸接触，并用至少第一荧光染料标记; （b）使样品中的核酸与不同于第一荧光染料的第二荧光染料接触，使得第二荧光染料与核酸相互作用; （c）如果存在于样品中则扩增核酸; 以及（d）通过在寡核苷酸与扩增的核酸杂交后通过观察来自第一荧光染料的荧光检测样品中的核酸，并通过测量第二荧光染料的荧光来测定扩增的核酸的解链温度。 第二荧光染料可以包括荧光嵌入剂。