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公开(公告)号：US20080201801A1
公开(公告)日：2008-08-21
申请号：US12033178
申请日：2008-02-19
申请人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
发明人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.
摘要翻译： 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。
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公开(公告)号：US08410334B2
公开(公告)日：2013-04-02
申请号：US12033178
申请日：2008-02-19
申请人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
发明人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest, recombinant DNA constructs for expression of at least one invertebrate miRNA in planta and methods of use thereof, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention.
摘要翻译： 本发明提供了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫有关的共生体的靶基因，用于将至少一种无脊椎动物miRNA表达的重组DNA构建体 植物及其使用方法，含有本发明的非天然转基因植物细胞的非天然转基因植物，从本发明的非天然转基因植物细胞生长的非天然转基因植物和非天然转基因种子 由非天然转基因植物产生，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。
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公开(公告)号：US08946511B2
公开(公告)日：2015-02-03
申请号：US11974469
申请日：2007-10-12
申请人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
发明人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
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4.发明申请公开(公告)号：US20110035838A1
公开(公告)日：2011-02-10
申请号：US12832938
申请日：2010-07-08
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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5.发明申请公开(公告)号：US20090013434A1
公开(公告)日：2009-01-08
申请号：US11544953
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael H. Luethy , Philip W. Miller , Larry A. Gilbertson , Edwards Allen , Sara E. Heisel , David K. Kovalic , James K. Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael H. Luethy , Philip W. Miller , Larry A. Gilbertson , Edwards Allen , Sara E. Heisel , David K. Kovalic , James K. Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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6.发明申请公开(公告)号：US20080229456A1
公开(公告)日：2008-09-18
申请号：US11545075
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael H. Luethy , Philip W. Miller , Larry A. Gilbertson , Edwards Allen , Sara E. Heisel , David K. Kovalic , James K. Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael H. Luethy , Philip W. Miller , Larry A. Gilbertson , Edwards Allen , Sara E. Heisel , David K. Kovalic , James K. Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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7.发明授权Engineered miRNA precursors for suppressing target gene expression in plants 有权用于抑制植物中靶基因表达的工程miRNA前体公开(公告)号：US07723568B2
公开(公告)日：2010-05-25
申请号：US11545106
申请日：2006-10-10
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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公开(公告)号：US20110035839A1
公开(公告)日：2011-02-10
申请号：US12832942
申请日：2010-07-08
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Leuthy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Leuthy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20090070898A1
公开(公告)日：2009-03-12
申请号：US11974469
申请日：2007-10-12
申请人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
发明人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
摘要翻译： 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。
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公开(公告)号：US20120192317A1
公开(公告)日：2012-07-26
申请号：US13305688
申请日：2011-11-28
申请人： GREGORY R. HECK , Tichafa R. I. Munyikwa , Jean C. Goley , James K. Roberts , Scott C. Johnson , Ty T. Vaughn
发明人： GREGORY R. HECK , Tichafa R. I. Munyikwa , Jean C. Goley , James K. Roberts , Scott C. Johnson , Ty T. Vaughn
CPC分类号： C12N15/113 , C12N15/111 , C12N15/8218 , C12N15/8286 , C12N2310/14 , C12N2320/10 , Y02A40/162
摘要： The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.
摘要翻译： 本发明提供了选择在目标生物体中产生dsRNA介导的基因抑制并使其能够被靶生物吸收的核苷酸序列的方法。 本发明进一步提供赋予转基因宿主细胞中此类序列稳定表达的表达构建体及其使用方法。 还提供了通过本发明的方法制备的生物，细胞和组织。
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