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公开(公告)号：US06406913B1
公开(公告)日：2002-06-18
申请号：US08471130
申请日：1995-06-06
IPC分类号： G01T1161
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。 还公开了组合物和试剂盒。
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公开(公告)号：US06251581B1
公开(公告)日：2001-06-26
申请号：US07704569
申请日：1991-05-22
IPC分类号： C12Q100
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。 还公开了组合物和试剂盒。
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公开(公告)号：US5340716A
公开(公告)日：1994-08-23
申请号：US718490
申请日：1991-06-20
IPC分类号： G01N33/569 , G01N33/58 , C12Q1/00
CPC分类号： G01N33/582 , G01N33/56977 , G01N33/58 , G01N33/585
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises providing (1) a medium suspected of containing the analyte, (2) a label reagent comprising a first specific binding pair (sbp) member associated with a photochemically activatable chemiluminescent compound wherein the first sbp member is capable of binding to the analyte or to a second sbp member to form a complex related to the presence of the analyte. The method further comprises photochemically activating the chemiluminescent compound. The amount of luminescence generated by the chemiluminescent compound is detected. The amount thereof is related to the amount of analyte in the medium. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括提供（1）怀疑含有分析物的培养基，（2）标记试剂，其包含与光化学活化的化学发光化合物相关的第一特异性结合对（sbp）成员，其中第一sbp成员能够结合分析物 或第二sbp成员以形成与分析物的存在有关的复合物。 该方法还包括光化学活化化学发光化合物。 检测由化学发光化合物产生的发光量。 其量与培养基中分析物的量有关。 还公开了组合物和试剂盒。
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公开(公告)号：US5618732A
公开(公告)日：1997-04-08
申请号：US434617
申请日：1995-05-04
IPC分类号： G01N33/532 , G01N21/78 , G01N33/58 , G01N21/64 , G01N21/76
CPC分类号： G01N33/582 , Y10S435/81 , Y10S436/829 , Y10S436/905 , Y10T436/10
摘要： Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
摘要翻译： 公开了标记材料的方法。 所述方法包括与材料（a）结合，所述光敏剂能够在产生单线态氧的照射时和（b）能够被单线态氧活化的化学发光化合物，其中光敏剂和化学发光化合物掺入颗粒基质或非均相中， 颗粒状固体基质。 颗粒基质可以是固体或流体。 这些方法允许产生延迟发光，这可以在照射基质时实现。 该方法可用于确定怀疑含有分析物的介质中的分析物。 一种方法包括使怀疑含有分析物的培养基相对于分析物的存在形成特异性结合对（sbp）成员的复合物的条件，以及通过使用标记a来确定sbp成员复合物是否形成 具有化学发光和光敏剂性质的单一组合物。 激活光敏剂时，产生单线态氧并激活化学发光性能。 还公开了组合物和试剂盒。
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公开(公告)号：US5709994A
公开(公告)日：1998-01-20
申请号：US470862
申请日：1995-06-06
CPC分类号： G01N33/582 , Y10S435/81 , Y10S436/829 , Y10S436/905 , Y10T436/10
摘要： Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
摘要翻译： 公开了标记材料的方法。 所述方法包括与材料（a）结合，所述光敏剂能够在产生单线态氧的照射时和（b）能够被单线态氧活化的化学发光化合物，其中光敏剂和化学发光化合物掺入颗粒基质或非均相中， 颗粒状固体基质。 颗粒基质可以是固体或流体。 这些方法允许产生延迟发光，这可以在照射基质时实现。 该方法可用于确定怀疑含有分析物的介质中的分析物。 一种方法包括使怀疑含有分析物的培养基相对于分析物的存在形成特异性结合对（sbp）成员的复合物的条件，以及通过使用标记a来确定sbp成员复合物是否形成 具有化学发光和光敏剂性质的单一组合物。 激活光敏剂时，产生单线态氧并激活化学发光性能。 还公开了组合物和试剂盒。
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公开(公告)号：US06284546B1
公开(公告)日：2001-09-04
申请号：US08469578
申请日：1995-06-06
IPC分类号： G01N2176
CPC分类号： G01N21/8806 , B01F5/0085 , B01F11/00 , B01F13/0001 , B01F13/0013 , B01F13/0809 , B01F15/0201 , B01F2215/0037 , B01L3/5085 , B01L2400/0415 , B01L2400/0433 , G01N21/8483 , G01N2001/386 , G01N2035/00237 , G01N2035/00554 , Y10T436/110833 , Y10T436/25
摘要： The present invention provides methods and apparatus for mixing two or more liquids and detecting light emitted by the mixture. The method comprises forming a liquid droplet containing two or more liquids on a substantially planar surface in containerless containment on the surface and causing the droplet to deform in an essentially zero air flow environment thereby mixing the liquids and surrounding the planar surface with a reflective housing and photodetector. An apparatus of the invention comprises (a) a substantially planar support, (b) means for dispensing liquids onto the support to form a droplet, and (c) non-evaporative means for causing the droplet to deform without deforming the support thereby mixing the liquids and a two part reflective housing and photodetector which is moveable. The drop can be deformed by, for example, application of acoustic energy or a variable electrostatic field. The methods and apparatus have particular application to the determination of an analyte.
摘要翻译： 本发明提供了混合两种或多种液体并检测由混合物发射的光的方法和装置。 该方法包括在表面上的无容器容纳物中的基本上平坦的表面上形成含有两种或更多种液体的液滴，并使液滴在基本为零的空气流动环境中变形，从而混合液体并围绕平面表面与反射壳体 光电探测器 本发明的装置包括（a）基本上平面的支撑件，（b）用于将液体分配到支撑件上以形成液滴的装置，和（c）非蒸发装置，用于使液滴变形而不使支架变形， 液体和可移动的两部分反射壳体和光电检测器。 可以通过例如施加声能或可变静电场来使液滴变形。 所述方法和装置特别适用于分析物的测定。
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公开(公告)号：US4891324A
公开(公告)日：1990-01-02
申请号：US925
申请日：1987-01-07
申请人： John Pease , Litai Weng , Hrair Kirakossian , Edwin F. Ullman
发明人： John Pease , Litai Weng , Hrair Kirakossian , Edwin F. Ullman
IPC分类号： G01N33/543 , C09B23/01 , C09B57/00 , G01N33/544 , G01N33/58
CPC分类号： C09B23/0066 , C09B57/007 , G01N33/582 , G01N33/585 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10S436/809 , Y10S436/821 , Y10S436/823 , Y10S436/829 , Y10T428/2982
摘要： Assay methods are provided for determining an analyte in a sample suspected of containing the analyte. The method is carried out using a composition that includes a conjugate of a first sbp member with a particle. A luminescer is reversibly associated with a nonaqueous phase of the particle. Where the first sbp member is not complementary to the analyte, a second sbp member that is capable of binding to the first sbp member is employed. Unbound conjugate is separated from conjugate that is bound to the analyte or to the second sbp member. A reagent for enhancing the detectability of the luminescer is added and the light emission of the luminescer acted on by the reagent is measured.
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公开(公告)号：US07179660B1
公开(公告)日：2007-02-20
申请号：US10220623
申请日：2000-03-06
申请人： Hrair Kirakossian , John S. Pease , Carsten Schelp , Marcel R. Pirio , Uwe Stöhr , Andreas Wiegand
发明人： Hrair Kirakossian , John S. Pease , Carsten Schelp , Marcel R. Pirio , Uwe Stöhr , Andreas Wiegand
IPC分类号： G01N33/548
CPC分类号： G01N33/548 , Y10S435/969 , Y10S436/827 , Y10T428/2991
摘要： A polysaccharide coated carrier having a coating of at least two successive layers of polysaccharide. The first polysaccharide layer spontaneously associates with a second polysaccharide layer and, optionally, the carrier. Each successive layer of polysaccharide spontaneously associates with a preceding layer. Spontaneous association occurs due to the presence of oppositely charged functional groups on each layer of polysaccharide or due to a spontaneous reaction between the functional groups the layers. The carrier may be any surface such as a tube, microtitration plate, bead, particle or the like and is suitable for use in diagnostic or therapeutic methods.
摘要翻译： 具有至少两层连续多层多糖的涂层的多糖涂覆的载体。 第一多糖层自发地与第二多糖层和任选的载体结合。 每个连续的多糖多层自发地与前一层相关联。 由于在多层多层上存在相反电荷的官能团，或由于各层之间的官能团之间的自发反应，发生自发缔合。 载体可以是诸如管，微量滴定板，珠粒，颗粒等的任何表面，并且适用于诊断或治疗方法。
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9.发明授权Methods and compositions for cytometric detection of rare target cells in a sample 有权样品中稀有靶细胞的细胞检测方法和组成公开(公告)号：US09535065B2
公开(公告)日：2017-01-03
申请号：US14237856
申请日：2012-09-06
IPC分类号： G01N33/533 , G01N33/569 , G01N33/574 , G01N15/14
CPC分类号： G01N33/56966 , G01N15/1459 , G01N33/533 , G01N33/56972 , G01N33/57484 , G01N33/57492 , G01N33/57496 , G01N2015/1402 , G01N2015/1477
摘要： The present disclosure provides cytometric methods for the detection of rare target cells in a sample. In certain aspects, the methods and compositions may facilitate the detection of rare target cells, such as circulating tumor cells (CTCs), in a biological sample such as blood. Aspects of the methods include contacting the sample with first and second binding members that specifically bind to a marker of the rare target cell, and cytometrically assaying the sample for the presence of cells comprising bound first and second binding members to detect the rare target cell in the sample. Also provided are systems, compositions, and kits for practicing the subject methods.
摘要翻译： 本公开提供了用于检测样品中罕见靶细胞的细胞计数方法。 在某些方面，所述方法和组合物可有助于在诸如血液的生物样品中检测稀有靶细胞，例如循环肿瘤细胞（CTC）。 方法的方面包括将样品与特异性结合罕见靶细胞标记物的第一和第二结合成员接触，以及细胞测定样品中含有结合的第一和第二结合成员的细胞的存在以检测罕见的靶细胞 例子。 还提供了用于实践主题方法的系统，组合物和试剂盒。
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公开(公告)号：US07537938B2
公开(公告)日：2009-05-26
申请号：US10765773
申请日：2004-01-26
申请人： Hrair Kirakossian , Thomas Klopack , Sharat Singh
发明人： Hrair Kirakossian , Thomas Klopack , Sharat Singh
IPC分类号： G01N33/553 , G01N33/53
CPC分类号： G01N33/54326 , G01N33/5091 , G01N33/566 , G01N33/574 , G01N33/6845
摘要： A method is provided for detecting biomarkers in rare circulating cells by forming an enriched population of cells immunomagnetically followed by biomarker detection using binding compounds having releasable molecular tags. Preferably, biomarkers comprising one or more protein-protein complexes are detected in circulating cancer cells metastasized from a solid tumor. In the presence of biomarkers, the molecular tags of the binding compounds are released and separated from the assay mixture for detection and/or quantification.
摘要翻译： 提供了一种用于通过使用具有可释放分子标签的结合化合物免疫磁性地形成富集的细胞群而进行生物标志物检测来检测稀有循环细胞中的生物标志物的方法。 优选地，在从实体瘤转移的循环癌细胞中检测到包含一种或多种蛋白质 - 蛋白质复合物的生物标志物。 在存在生物标志物的情况下，将结合化合物的分子标签从测定混合物中释放并分离出来用于检测和/或定量。
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