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公开(公告)号：US06406913B1
公开(公告)日：2002-06-18
申请号：US08471130
申请日：1995-06-06
IPC分类号： G01T1161
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。 还公开了组合物和试剂盒。
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公开(公告)号：US06251581B1
公开(公告)日：2001-06-26
申请号：US07704569
申请日：1991-05-22
IPC分类号： C12Q100
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。 还公开了组合物和试剂盒。
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公开(公告)号：US5340716A
公开(公告)日：1994-08-23
申请号：US718490
申请日：1991-06-20
IPC分类号： G01N33/569 , G01N33/58 , C12Q1/00
CPC分类号： G01N33/582 , G01N33/56977 , G01N33/58 , G01N33/585
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises providing (1) a medium suspected of containing the analyte, (2) a label reagent comprising a first specific binding pair (sbp) member associated with a photochemically activatable chemiluminescent compound wherein the first sbp member is capable of binding to the analyte or to a second sbp member to form a complex related to the presence of the analyte. The method further comprises photochemically activating the chemiluminescent compound. The amount of luminescence generated by the chemiluminescent compound is detected. The amount thereof is related to the amount of analyte in the medium. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括提供（1）怀疑含有分析物的培养基，（2）标记试剂，其包含与光化学活化的化学发光化合物相关的第一特异性结合对（sbp）成员，其中第一sbp成员能够结合分析物 或第二sbp成员以形成与分析物的存在有关的复合物。 该方法还包括光化学活化化学发光化合物。 检测由化学发光化合物产生的发光量。 其量与培养基中分析物的量有关。 还公开了组合物和试剂盒。
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公开(公告)号：US5350855A
公开(公告)日：1994-09-27
申请号：US954790
申请日：1992-09-30
IPC分类号： C07D213/34 , C07D213/65 , C07D213/55 , C07D401/12
CPC分类号： C07D213/34 , C07D213/65 , Y10S436/819
摘要： O-acylated pyridinium compounds in which the 3-hydroxy moiety derivatized specifically at its aliphatic hydroxyl group by a selected acyl group is disclosed. In various embodiments, the composition may be used as a standard for HPLC or immunoassay of pyridinoline, a pyridinoline immunogen for producing anti-pyridinoline antibodies, and a solid-phase reagent for use in an immunoassay kit. Also disclosed are methods for making and using the composition.
摘要翻译： 公开了其中通过选择的酰基特异性地在其脂族羟基衍生的3-羟基部分的O-酰化的吡啶鎓化合物。 在各种实施方案中，组合物可以用作HPLC或免疫测定吡啶啉的标准品，用于产生抗吡啶啉啉抗体的吡啶啉免疫原和用于免疫测定试剂盒的固相试剂。 还公开了制备和使用组合物的方法。
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公开(公告)号：US5502197A
公开(公告)日：1996-03-26
申请号：US234068
申请日：1994-04-28
IPC分类号： C07D213/34 , C07D213/65 , C07D401/12 , C07D213/55 , C07D213/71
CPC分类号： C07D213/34 , C07D213/65 , Y10S436/819
摘要： A pyridinoline composition in which pyridinoline is derivatized specifically at its aliphatic hydroxyl group by a selected chemical group is disclosed. In various embodiments, the composition may be used as a standard for HPLC or immunoassay of pyridinoline, a pyridinoline immunogen for producing anti-pyridinoline antibodies, and a solid-phase reagent for use in an immunoassay kit. Also disclosed are methods for making and using the composition.
摘要翻译： 公开了吡啶啉组合物，其中吡啶啉通过选定的化学基团在其脂族羟基上特异性衍生。 在各种实施方案中，组合物可以用作HPLC或免疫测定吡啶啉的标准品，用于产生抗吡啶啉啉抗体的吡啶啉免疫原和用于免疫测定试剂盒的固相试剂。 还公开了制备和使用组合物的方法。
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公开(公告)号：US5756679A
公开(公告)日：1998-05-26
申请号：US620418
申请日：1996-03-22
IPC分类号： C07D213/34 , C07D213/65 , C07K14/765 , C07K14/805 , G01N33/68
CPC分类号： C07D213/34 , C07D213/65 , Y10S436/819
摘要： A pyridinoline composition in which pyridinoline is derivatized specifically at its aliphatic hydroxyl group by a selected chemical group is disclosed. In various embodiments, the composition may be used as a standard for HPLC or immunoassay of pyridinoline, a pyridinoline immunogen for producing anti-pyridinoline antibodies, and a solid-phase reagent for use in an immunoassay kit. Also disclosed are methods for making and using the composition.
摘要翻译： 公开了吡啶啉组合物，其中吡啶啉通过选定的化学基团在其脂族羟基上特异性衍生。 在各种实施方案中，组合物可以用作HPLC或免疫测定吡啶啉的标准品，用于产生抗吡啶啉啉抗体的吡啶啉免疫原和用于免疫测定试剂盒的固相试剂。 还公开了制作和使用组合物的方法。
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公开(公告)号：US5527715A
公开(公告)日：1996-06-18
申请号：US208871
申请日：1994-03-10
申请人： Viola T. Kung , Mary J. Cerelli , Yuri Daniloff , Robert F. Zuk
发明人： Viola T. Kung , Mary J. Cerelli , Yuri Daniloff , Robert F. Zuk
CPC分类号： G01N33/6887 , C07K16/44 , G01N33/5308 , Y10S435/962
摘要： A method of assaying a human urine sample, by measuring a concentration of pyridinium crosslinks from which the concentration of total hydrolysed pyridinoline in the sample can be determined, is disclosed. In the method, a urine sample is reacted with an anti-Pyd antibody reagent which preferably has a ratio of reactivity toward native free pyridinoline and urinary pyridinoline peptides larger than 1,000 daltons in molecular weight, of greater than 10:1. By measuring the extent of immunocomplex formed by the reaction, the concentration of total hydrolysed pyridinoline in the sample can be calculated. Also disclosed are an antibody reagent and kit which can be used in the method.
摘要翻译： 公开了一种通过测量可以确定样品中总水解吡啶啉的浓度的吡啶鎓交联的浓度来测定人类尿样的方法。 在该方法中，尿样与抗Pyd抗体试剂反应，其优选对天然游离吡啶啉的反应性与分子量大于1,000道尔顿的尿吡啶啉肽的比例大于10:1。 通过测量由反应形成的免疫复合物的程度，可以计算样品中总水解的吡啶啉的浓度。 还公开了可用于该方法的抗体试剂和试剂盒。
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