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    Method for preparation of a recombinant DNA phage
    1.
    发明授权
    Method for preparation of a recombinant DNA phage 失效
    重组DNA噬菌体的制备方法

    公开(公告)号：US4348478A

    公开(公告)日：1982-09-07

    申请号：US87167

    申请日：1979-10-22

    申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

    发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

    IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12R1/91

    CPC分类号： C12N15/00

    摘要： A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.

    摘要翻译： 一种制备新的重组DNA的方法，其包括（1）用核酸内切酶切割温和的噬菌体DNA，所述温带噬菌体DNA具有参与噬菌体DNA复制的DNA片段中不具有内切核酸酶敏感区域，并将噬菌体DNA整合到 宿主染色体，但至少在DNA片段中携带外壳蛋白生产的遗传信息和携带预期遗传信息的另一个DNA，（2）向裂解DNA的混合物中加入DNA连接酶，和（3）从混合物中回收噬菌体 通过用携带预期信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段，缺失其外壳蛋白产生能力的DNA。

    Method for preparing a recombinant DNA phage
    2.
    发明授权
    Method for preparing a recombinant DNA phage 失效
    制备重组DNA噬菌体的方法

    公开(公告)号：US4348477A

    公开(公告)日：1982-09-07

    申请号：US87166

    申请日：1979-10-22

    申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

    发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

    IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12R1/91

    CPC分类号： C12N15/00

    摘要： A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.

    摘要翻译： 一种制备新型重组DNA的方法，其包括（1）用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA，其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中，但在其他DNA （2）将由所述切割产生的所有片段混合在一起，（3）将所述切割产生的所有片段混合在一起，其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA， 向混合物中加入DNA连接酶，（4）从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA，通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。

    Novel bacteriophage and method for preparing same
    3.
    发明授权
    Novel bacteriophage and method for preparing same 失效
    新型噬菌体及其制备方法

    公开(公告)号：US4332897A

    公开(公告)日：1982-06-01

    申请号：US895602

    申请日：1978-04-12

    申请人： Eiichi Nakano ,  Narimasa Saito ,  Danji Fukushima

    发明人： Eiichi Nakano ,  Narimasa Saito ,  Danji Fukushima

    IPC分类号： C12N15/09 ,  C12N7/00 ,  C12N15/00 ,  C12R1/91

    CPC分类号： C12N15/00

    摘要： A novel bacteriophage whose DNA molecule has endonuclease-sensitivity only in the DNA region carrying genetic information for the production of phage coat proteins can be obtained by isolating an endonuclease-resistant mutant from one of the lambdoid bacteriophages and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins.

    摘要翻译： 一种新的噬菌体，其DNA分子仅在携带用于产生噬菌体外壳蛋白的遗传信息的DNA区域中具有核酸内切酶敏感性，可以通过从其中一个羊瘟噬菌体中分离内切核酸酶抗性突变体并将所得噬菌体与羊瘟噬菌体 在携带用于产生外壳蛋白的遗传信息的DNA区域中具有核酸内切酶敏感性。

    Purified luciferase from luciola lateralis
    4.
    发明授权
    Purified luciferase from luciola lateralis 失效
    来自luciola lateralis的纯化萤光素酶

    公开(公告)号：US5352598A

    公开(公告)日：1994-10-04

    申请号：US759814

    申请日：1991-08-29

    申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N1/00

    CPC分类号： C12N9/0069 ,  C12Y113/12007 ,  Y10S435/815 ,  Y10S435/816

    摘要： A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

    摘要翻译： 公开了一种来自Luciola lateralis的纯化的萤光素酶。 该酶的特征在于具有以下特性：最佳pH范围为7.5至9.5，最适温度范围为0℃至50℃，并且该酶不作用于ADP，CTP，UTP和GTP。 通过使用包括凝胶过滤色谱法，羟基磷灰石柱色谱法和三（羟基）氨基甲烷 - 氢氯酸缓冲液的方法纯化酶。

    Novel bacteriophage and method for breeding thereof
    7.
    发明授权
    Novel bacteriophage and method for breeding thereof 失效
    新型噬菌体及其育种方法

    公开(公告)号：US4865979A

    公开(公告)日：1989-09-12

    申请号：US498802

    申请日：1983-05-27

    申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Yasuji Koyama ,  Satoshi Kitao

    发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Yasuji Koyama ,  Satoshi Kitao

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N7/00 ,  C12N15/00 ,  C12N15/70 ,  C12N15/73 ,  C12R1/91

    CPC分类号： C12N15/70 ,  C12N15/73

    摘要： A bacteriophage which is characterized by the presence of one or more restriction enzyme sites downstream from the late promoter up to the cohesive end, and none or a permissible number of restriction enzyme sites upstream from the late promoter to the cohesive end is useful as an expression vector. The vector can be used to obtain high expression of DNA inserted at the restriction enzyme site downstream from the late promoter up to the cohesive end.

    摘要翻译： 一种噬菌体，其特征在于存在一个或多个限制酶位点，从晚期启动子下游到粘性末端，并且没有或允许数量的限制酶位点从后期启动子上游到粘性末端可用作表达 向量。 该载体可用于获得插入在晚期启动子下游的限制酶位点直至粘性末端的DNA的高表达。

    Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex
    8.
    发明授权
    Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex 失效
    丙酮酸脱氢酶复合物的变体E1蛋白基因和丙酮酸脱氢酶复合体的变体E1蛋白

    公开(公告)号：US5432071A

    公开(公告)日：1995-07-11

    申请号：US215709

    申请日：1994-03-22

    申请人： Toshio Ichikawa ,  Yasuji Koyama ,  Hideko Otake ,  Eiichi Nakano

    发明人： Toshio Ichikawa ,  Yasuji Koyama ,  Hideko Otake ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N15/09 ,  C12R1/19 ,  C12N9/04 ,  C12N15/53 ,  C12N15/70

    CPC分类号： C12N9/0008 ,  C12Y102/04001 ,  C12Y102/05001

    摘要： The present invention relates to variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of pyruvate dehydrogenase complex, a gene coding for said variant E.sub.1 protein, a novel recombinant DNA comprising said variant E.sub.1 protein-encoding gene inserted in a vector DNA, and a process for producing variant E.sub.1 protein by said recombinant DNA. The present invention provides the variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity.

    摘要翻译： 本发明涉及高活性的丙酮酸脱氢酶复合物的变体E1蛋白，其中146位的精氨酸被丙酮酸脱氢酶复合物的野生型E1蛋白的氨基酸序列中的脯氨酸替代，编码所述变体E1的基因 蛋白质，包含插入载体DNA中的所述变体E1蛋白质编码基因的新型重组DNA，以及由所述重组DNA产生变体E1蛋白质的方法。 本发明提供了具有高活性的丙酮酸脱氢酶复合物的变体E1蛋白。

    Method of controlling electrocoating bath and apparatus therefor
    9.
    发明授权
    Method of controlling electrocoating bath and apparatus therefor 失效
    电泳浴的控制方法及其设备

    公开(公告)号：US4501649A

    公开(公告)日：1985-02-26

    申请号：US548139

    申请日：1983-11-02

    申请人： Nobuo Furuno ,  Yoshio Ota ,  Masahiro Itai ,  Akio Tokuyama ,  Eiichi Nakano

    发明人： Nobuo Furuno ,  Yoshio Ota ,  Masahiro Itai ,  Akio Tokuyama ,  Eiichi Nakano

    IPC分类号： C25D13/24

    CPC分类号： C25D13/24

    摘要： The present invention provides a method of controlling an electrocoating bath using an ion exchange resin. The ion exchange resin is maintained in a porous container in a suspended state in the electrocoating bath, and is used in a quantity of not more than the chemical equivalent of excess counter-ion to be removed from the bath. Bath control is easily achieved without any coagulation trouble as often observed in conventional column methods.

    摘要翻译： 本发明提供一种使用离子交换树脂控制电泳浴的方法。 离子交换树脂在电镀浴中以悬浮状态保持在多孔容器中，并且使用的量不超过要从浴中除去的过量抗衡离子的化学当量。 在常规柱方法中经常观察到的浴控制容易实现而没有任何凝结问题。

    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter
    10.
    发明授权
    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter 失效
    含有鞣酸酶基因的DNA片段，重组质粒，鞣酸酶的制备方法和启动子

    公开(公告)号：US5665584A

    公开(公告)日：1997-09-09

    申请号：US460860

    申请日：1995-06-05

    申请人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    发明人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N9/18 ,  C12N15/00 ,  C12R1/69

    CPC分类号： C12N9/18

    摘要： The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.

    摘要翻译： 本发明涉及含有编码鞣酸酶的基因并衍生自属于曲霉属的微生物的3,563个碱基对的DNA片段，其具有以下限制酶图：B：Bam HI，H：Hind III，K ：Kpn I，S：Sal I，X：Xba I; 含有编码（SEQ ID NO：4）的氨基酸序列的鞣酸酶基因的DNA片段; 包含插入质粒载体中的含有鞣酸酶基因的DNA片段的重组质粒; 一种鞣酸酶的制造方法，其特征在于，在培养基中培养能够在培养基中生产鞣酸酶的微生物属于属于曲霉属的微生物，从培养物中回收鞣酸酶; 和由（SEQ ID NO：1）的核苷酸序列表示的启动子。 根据本发明可以有效地制备鞣酸酶。