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    Expression cloning methods in filamentous fungi
    3.
    发明申请
    Expression cloning methods in filamentous fungi 审中-公开
    丝状真菌中的表达克隆方法

    公开(公告)号：US20050170350A1

    公开(公告)日：2005-08-04

    申请号：US10504646

    申请日：2003-02-18

    申请人： Mary Stringer ,  Kirk Schnorr ,  Jesper Vind

    发明人： Mary Stringer ,  Kirk Schnorr ,  Jesper Vind

    IPC分类号： C12N15/65 ,  C12N15/80 ,  C12Q1/68 ,  C12N1/16 ,  C12N15/74 ,  G01N33/53

    CPC分类号： C12N15/65 ,  C12N15/80

    摘要： Methods for screening a polynucleotide library for a polypeptide with a property of interest in a filamentous fungal host cell, in a manner which allows quick and easy subsequent characterization of the polypeptide, using an expression cloning vector comprising at least a polynucleotide encoding a selectable marker in which the translation initiation start site of the marker-encoding sequence comprises a crippled consensus Kozak sequence, a fungal replication initiation sequence, and a promoter with a cloning-site into which the library is cloned, and a transcription terminator.

    摘要翻译： 使用包含至少编码可选择标记的多核苷酸的表达克隆载体以允许快速且容易地随后表征多肽的方式筛选丝状真菌宿主细胞中具有感兴趣性质的多肽的多核苷酸文库的方法 其标记编码序列的翻译起始起始位点包含残基共有的Kozak序列，真菌复制起始序列和具有克隆文库的克隆位点的启动子和转录终止子。

    Signal sequence trapping
    6.
    发明申请
    Signal sequence trapping 审中-公开
    信号序列陷阱

    公开(公告)号：US20080058214A1

    公开(公告)日：2008-03-06

    申请号：US11341754

    申请日：2006-01-27

    申请人： Fiona Duffner ,  Reinhard Wilting ,  Kirk Schnorr

    发明人： Fiona Duffner ,  Reinhard Wilting ,  Kirk Schnorr

    IPC分类号： C40B20/00

    CPC分类号： C12N15/1034

    摘要： The present invention allows the screening of previously established genebanks or libraries by proxy for genes encoding secreted, partially secreted, or cell surface-displayed polypeptides of industrial interest, such as enzymes, receptors, cytokines, peptide hormones etc. that would not likely have been isolated using conventional screening assays. A method for isolating genes encoding secreted, partially secreted, or cell surface displayed polypeptides from existing gene libraries is described in which the endogenous secretion signal sequences are detected using an in vitro polynucleotide insertion reaction where the inserted polynucleotide comprises a promoter-less and secretion signal-less secretion reporter.

    摘要翻译： 本发明允许通过代理方法筛选先前建立的基因库或文库，用于编码分泌的，部分分泌的或表面活性的细胞表达多肽，例如酶，受体，细胞因子，肽荷尔蒙等不可能已经被 使用常规筛选测定分离。 描述了从现有基因文库分离编码分泌的，部分分泌的或细胞表面展示的多肽的基因的方法，其中使用体外多核苷酸插入反应检测内源性分泌信号序列，其中插入的多核苷酸包含无启动子和分泌信号 无分泌记者。

    Signal sequence trapping
    10.
    发明授权
    Signal sequence trapping 失效

    公开(公告)号：US07029842B2

    公开(公告)日：2006-04-18

    申请号：US09823825

    申请日：2001-03-30

    申请人： Fiona Duffner ,  Reinhard Wilting ,  Kirk Schnorr

    发明人： Fiona Duffner ,  Reinhard Wilting ,  Kirk Schnorr

    IPC分类号： C12Q1/00 ,  C12P19/34 ,  C12N15/00 ,  C07H21/02

    CPC分类号： C12N15/1034

    摘要： The present invention allows the screening of previously established genebanks or libraries by proxy for genes encoding secreted, partially secreted, or cell surface-displayed polypeptides of industrial interest, such as enzymes, receptors, cytokines, peptide hormones etc. that would not likely have been isolated using conventional screening assays. A method for isolating genes encoding secreted, partially secreted, or cell surface displayed polypeptides from existing gene libraries is described in which the endogenous secretion signal sequences are detected using an in vitro polynucleotide insertion reaction where the inserted polynucleotide comprises a promoter-less and secretion signal-less secretion reporter.