利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

28 条记录 (耗时 0.04 秒)

    Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
    3.
    发明授权
    Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms 失效
    在真核生物体内操作原卟啉原氧化酶活性

    公开(公告)号：US06177245B1

    公开(公告)日：2001-01-23

    申请号：US09071296

    申请日：1998-05-01

    申请人： Eric R. Ward ,  Sandra Volrath

    发明人： Eric R. Ward ,  Sandra Volrath

    IPC分类号： C12Q168

    CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

    摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants may be bred or engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or through increased levels of expression of the native protox gene, or they may be transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Diagnostic and other uses for the novel eukaryotic protox sequence are also described. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

    摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物可以通过将天然protox基因突变为抗性形式或通过增加天然protox基因的表达水平培育或设计用于抵抗前毒素抑制剂，或者可以用经修饰的真核或原核protox编码序列或野生型 类型的原核生殖毒素序列是耐除草剂。 还描述了用于新型真核毒素序列的诊断和其它用途。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

    Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
    4.
    发明授权
    Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms 失效
    在真核生物体内操作原卟啉原氧化酶活性

    公开(公告)号：US5767373A

    公开(公告)日：1998-06-16

    申请号：US472028

    申请日：1995-06-06

    申请人： Eric R. Ward ,  Sandra Volrath

    发明人： Eric R. Ward ,  Sandra Volrath

    IPC分类号： G01N33/566 ,  A01H1/04 ,  A01H5/00 ,  A01N43/38 ,  A01N43/54 ,  A01N43/56 ,  A01N43/653 ,  A01N43/824 ,  A01N43/836 ,  A01N43/84 ,  A61K38/44 ,  A61K48/00 ,  A61P43/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/04 ,  C12N5/10 ,  C12N5/14 ,  C12N9/02 ,  C12N15/09 ,  C12N15/53 ,  C12N15/82 ,  C12Q1/26 ,  C12Q1/68 ,  C12R1/91 ,  C12N15/00

    CPC分类号： C12Y103/03004 ,  C12N15/8274 ,  C12N5/04 ,  C12N9/001 ,  C12Q1/6895

    摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants may be bred or engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or through increased levels of expression of the native protox gene, or they may be transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Diagnostic and other uses for the novel eukaryotic protox sequence are also described. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

    摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物可以通过将天然protox基因突变为抗性形式或通过增加天然protox基因的表达水平培育或设计用于抵抗前毒素抑制剂，或者可以用经修饰的真核或原核protox编码序列或野生型 类型的原核生殖毒素序列是耐除草剂。 还描述了用于新型真核毒素序列的诊断和其它用途。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

    Herbicide tolerant plants, plant tissue or plant cells having altered protoporphyrinogen oxidase activity
    5.
    发明授权
    Herbicide tolerant plants, plant tissue or plant cells having altered protoporphyrinogen oxidase activity 失效
    除草剂耐受性植物，具有改变的原卟啉原氧化酶活性的植物组织或植物细胞

    公开(公告)号：US06307129B1

    公开(公告)日：2001-10-23

    申请号：US09191998

    申请日：1998-11-12

    申请人： Eric R. Ward ,  Sandra Volrath

    发明人： Eric R. Ward ,  Sandra Volrath

    IPC分类号： A01H500

    CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

    摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants are engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they are transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

    摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物通过将天然protox基因突变为抗性形式而被设计用于抵抗前毒素抑制剂，或者用修饰的真核或原核protox编码序列或耐除草剂的野生型原核protox序列转化。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

    Method for breeding disease resisteance into plants
    9.
    发明授权
    Method for breeding disease resisteance into plants 失效
    将病害抗性育种到植物中的方法

    公开(公告)号：US5792904A

    公开(公告)日：1998-08-11

    申请号：US648949

    申请日：1996-05-16

    申请人： John A. Ryals ,  Scott J. Uknes ,  Terrence Patrick Delaney ,  Eric R. Ward ,  Henry-York Steiner

    发明人： John A. Ryals ,  Scott J. Uknes ,  Terrence Patrick Delaney ,  Eric R. Ward ,  Henry-York Steiner

    IPC分类号： A01H1/00 ,  A01H1/02 ,  A01H1/04 ,  A61K49/00 ,  C07K14/415 ,  C12N9/24 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H1/06

    CPC分类号： C12N15/8281 ,  A01H1/02 ,  A01H1/04 ,  A61K49/0008 ,  C07K14/415 ,  C12N15/8282

    摘要： Methods are provided for selecting parental plants having disease resistance and for using these plants in breeding programs. In one method of the invention, lesion mimic mutants are screened for either resistance to a pathogen of interest or for the expression of systemic acquired resistance (SAR) genes. Such mutants having the desired traits or expressing the desired genes are then used in breeding programs. Parent plants can also be selected based on the constitutive expression of SAR genes. These mutants are phenotypically normal yet exhibit a significant level of disease resistance. Also disclosed are plant mutants that do not express systemic acquired resistance genes even when induced by a pathogen and methods of use for such mutants.

    摘要翻译： 提供了用于选择具有抗病性的亲本植物和在育种程序中使用这些植物的方法。 在本发明的一种方法中，筛选病毒模拟突变体对感兴趣的病原体的抗性或用于表达系统性获得性抗性（SAR）基因。 然后将具有所需性状或表达所需基因的这种突变体用于育种程序中。 也可以根据SAR基因的组成型表达选择亲本植物。 这些突变体在表型上是正常的，但具有显着水平的抗病性。 还公开了即使在由病原体诱导的情况下也不表达系统性获得性抗性基因的植物突变体和用于这种突变体的方法。

    Method of inducing gene transcription in a plant
    10.
    发明授权
    Method of inducing gene transcription in a plant 失效
    在植物中诱导基因转录的方法

    公开(公告)号：US5789214A

    公开(公告)日：1998-08-04

    申请号：US455244

    申请日：1995-05-31

    申请人： John A. Ryals ,  Leslie B. Friedrich ,  Scott J. Uknes ,  Eric R. Ward

    发明人： John A. Ryals ,  Leslie B. Friedrich ,  Scott J. Uknes ,  Eric R. Ward

    IPC分类号： C07D285/14 ,  C07K14/415 ,  C12N9/02 ,  C12N9/08 ,  C12N9/24 ,  C12N9/36 ,  C12N15/62 ,  C12N15/82 ,  C12N15/29 ,  C12N5/04 ,  A01H5/00

    CPC分类号： C07D285/14 ,  C07K14/415 ,  C12N15/625 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8282 ,  C12N15/8286 ,  C12N9/0065 ,  C12N9/0073 ,  C12N9/244 ,  C12N9/2442 ,  C12N9/2462 ,  C12Y114/13001 ,  C12Y302/01006 ,  C12Y302/01014 ,  C12Y302/01039 ,  C12Y302/01058 ,  C12Y302/01073 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/55 ,  C07K2319/61

    摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.

    摘要翻译： 本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。 在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。 本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。 还提供了外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物，因为至少一种内源性信号转导级联失活 其调节植物中的基因，并且在需要表达基因的时候将化学调节剂施用于植物。 还提供了新的信号肽序列和相应的DNA编码序列。 进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。