公开(公告)号：US09725746B2

公开(公告)日：2017-08-08

申请号：US14649414

申请日：2013-12-18

申请人： Evonik Degussa GmbH

发明人： Steffen Schaffer ,  Jasmin Corthals ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Michael Volland ,  Martin Roos

IPC分类号： C12P13/00 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N15/52

CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/1288 ,  C12N15/52 ,  C12Y102/01042 ,  C12Y104/01001 ,  C12Y113/11 ,  C12Y207/08007 ,  Y02P20/582

摘要： The invention relates to a whole-cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an α-dioxygenase and contacting the product with a transaminase.