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    METHODS AND COMPOSITIONS FOR LONG TERM HEMATOPOIETIC REPOPULATION
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR LONG TERM HEMATOPOIETIC REPOPULATION 审中-公开
    长期HEMATOPOIETIC REPOPULATION的方法和组合

    公开(公告)号：US20120114614A1

    公开(公告)日：2012-05-10

    申请号：US13129359

    申请日：2009-11-16

    申请人： Janina Ratajczak ,  Ewa K. Zuba-Surma ,  Mariusz Ratajczak

    发明人： Janina Ratajczak ,  Ewa K. Zuba-Surma ,  Mariusz Ratajczak

    IPC分类号： A61K35/12 ,  A61N5/00 ,  C12N5/02 ,  C12N5/078 ,  C12N5/07

    CPC分类号： A61K35/28 ,  A61K35/51 ,  A61K2035/124 ,  C12N5/0647 ,  C12N5/0665 ,  C12N2502/1335 ,  C12N2502/1394

    摘要： Methods for isolating a CD133+/CD45neg/GlyAneg subpopulation of umbilical cord blood cells are disclosed. In some embodiments, the methods include providing an initial population of umbilical cord blood cells; contacting the initial population of cells with a first antibody that is specific for CD133, a second antibody that is specific for CD45, and a third antibody that is specific for Glycophorin A (GIyA) under conditions sufficient to allow binding of each antibody to its target, if present, on each cell of the initial population of cells; and isolating a subpopulation of cells that are CD133+, CD45neg, and GlyAneg. Also provided are isolated populations of CD133+/GlyAneg/CD45neg stem cells isolated from cord blood, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for inducing hematopoietic competency in CD133+/GlyAneg/CD45neg stem cells, and cell culture systems that include CD133+/GIyAneg/CD45neg stem cells.

    摘要翻译： 公开了分离脐带血细胞的CD133 + / CD45neg / GlyAneg亚群的方法。 在一些实施方案中，所述方法包括提供脐带血细胞的初始群体; 使初始细胞群与第一抗体CD133特异性接触，第二抗体是CD45特异性的第二抗体，以及在足以允许每种抗体与靶标结合的条件下对Glycophorin A（Gly）特异性的第三抗体 如果存在，则在初始细胞群体的每个细胞上; 并分离CD133 +，CD45neg和GlyAneg细胞亚群。 还提供了从脐带血分离的CD133 + / GlyAneg / CD45neg干细胞的分离群体，用于重新培养受试者中的细胞类型的方法，用于骨髓移植的方法，在CD133 + / GlyAneg / CD45neg干细胞中诱导造血能力的方法和细胞培养系统 其包括CD133 + / GlyYneg / CD45neg干细胞。