公开(公告)号：US20150011422A1

公开(公告)日：2015-01-08

申请号：US14327960

申请日：2014-07-10

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： C12Q1/42

CPC分类号： G01N33/6848 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/569 ,  G01N33/58 ,  G01N33/6842 ,  G01N2333/91205 ,  G01N2333/916 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2560/00 ,  Y10S435/975

摘要： Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes

摘要翻译： 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶enyzmes）方便和准确地分析底物的翻译后修饰

公开(公告)号：US09874567B2

公开(公告)日：2018-01-23

申请号：US15013880

申请日：2016-02-02

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： C12Q1/42 ,  G01N33/68 ,  C12Q1/48 ,  G01N33/569 ,  G01N33/58

CPC分类号： G01N33/6848 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/569 ,  G01N33/58 ,  G01N33/6842 ,  G01N2333/91205 ,  G01N2333/916 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2560/00 ,  Y10S435/975

摘要： Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enzymes. Kits may include an element tag for directly tagging an affinity product that recognizes phosphorylated substrates, as well as a non-phosphorylated substrate or an expression plasmid.

公开(公告)号：US20170023583A1

公开(公告)日：2017-01-26

申请号：US15039797

申请日：2014-11-26

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： G01N33/68 ,  G01N33/58 ,  C07K1/13 ,  C12Q1/37

CPC分类号： G01N33/6848 ,  C07K1/13 ,  C12Q1/37 ,  G01N33/58 ,  G01N2333/95 ,  G01N2458/15

摘要： The present invention generally relates to methods for the detection of enzymes using elemental analysis.

摘要翻译： 本发明一般涉及使用元素分析检测酶的方法。

公开(公告)号：US20160231327A1

公开(公告)日：2016-08-11

申请号：US15013880

申请日：2016-02-02

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/569 ,  G01N33/58 ,  G01N33/6842 ,  G01N2333/91205 ,  G01N2333/916 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2560/00 ,  Y10S435/975

摘要： Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes

公开(公告)号：US09284598B2

公开(公告)日：2016-03-15

申请号：US14327960

申请日：2014-07-10

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： C12Q1/42 ,  C12Q1/48 ,  G01N33/569 ,  G01N33/58 ,  G01N33/68

CPC分类号： G01N33/6848 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/569 ,  G01N33/58 ,  G01N33/6842 ,  G01N2333/91205 ,  G01N2333/916 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2560/00 ,  Y10S435/975

摘要： Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.

摘要翻译： 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶酶）方便和准确地分析底物的翻译后修饰。

公开(公告)号：US10745743B2

公开(公告)日：2020-08-18

申请号：US16270904

申请日：2019-02-08

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/58 ,  H01J49/00

摘要： The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.

公开(公告)号：US10577648B2

公开(公告)日：2020-03-03

申请号：US14823980

申请日：2015-08-11

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： G01N33/00 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  H01J49/00

摘要： The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.

公开(公告)号：US20140323339A1

公开(公告)日：2014-10-30

申请号：US14327948

申请日：2014-07-10

申请人： FLUIDIGM CANADA INC.

发明人： Olga Ornatsky

IPC分类号： C12Q1/48 ,  G01N33/68 ,  G01N33/58 ,  C12Q1/42

CPC分类号： G01N33/6848 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/569 ,  G01N33/58 ,  G01N33/6842 ,  G01N2333/91205 ,  G01N2333/916 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2560/00 ,  Y10S435/975

摘要： Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes

摘要翻译： 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶enyzmes）方便和准确地分析底物的翻译后修饰

公开(公告)号：US20190161790A1

公开(公告)日：2019-05-30

申请号：US16270904

申请日：2019-02-08

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： C12Q1/6825 ,  G01N33/58 ,  C12Q1/6816 ,  G01N33/543 ,  C12Q1/6841 ,  G01N33/68 ,  H01J49/00

摘要： The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.

公开(公告)号：US20180188264A1

公开(公告)日：2018-07-05

申请号：US15740527

申请日：2016-06-29

申请人： Fluidigm Canada Inc.

发明人： Olga Ornatsky

IPC分类号： G01N33/68 ,  C12Q1/6872 ,  C12Q1/6818

CPC分类号： G01N33/6851 ,  C07H21/00 ,  C07H21/02 ,  C12N2310/3145 ,  C12N2310/315 ,  C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6872 ,  C12Q2565/627 ,  C12Q2600/16

摘要： In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described.