公开(公告)号：US09725766B1

公开(公告)日：2017-08-08

申请号：US14588319

申请日：2014-12-31

Applicant: Flagship Biosciences, LLC

Inventor： Steven J. Potts

IPC: G06K9/00 ,  C12Q1/68 ,  G01N33/569

CPC classification number: G01N33/56966 ,  G01N33/5044 ,  G01N33/5064 ,  G01N33/56972 ,  G01N2800/7014 ,  G01N2800/7038 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30101

Abstract: The instant disclosure describes a method for measuring and reporting vascularity in a biological tissue sample. The method generally includes: within a digital image of a tissue section, (i) identifying endothelial cells, lymphatic cells, or a combination thereof; (ii) mapping one or more proximity regions, each of the proximity regions defining an area between detected vessels and a first distance outwardly therefrom; and (iii) calculating one or more of: a vessel proximity score or a hypoxia score, wherein the vessel proximity score relates a composition of objects within the proximity regions, and wherein the hypoxia score relates a composition of tissue within or outside of the proximity regions, respectively.