Process for coupling antibodies or antibody fragments to liposomes
    10.
    发明授权
    Process for coupling antibodies or antibody fragments to liposomes 失效
    将抗体或抗体片段与脂质体偶联的方法

    公开(公告)号:US5210040A

    公开(公告)日:1993-05-11

    申请号:US485395

    申请日:1990-02-22

    摘要: The present invention provides for novel homogeneous immunoassay systems involving complement-mediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. These systems do not require the separation of unbound antigens and/or antibody conjugates yet provide highly sensitive procedures for analyte detection. Liposomes containing a marker, are coupled to antibody fragments in a way which confers the liposomes with immunological specificity yet avoids sensitizing the liposomes to complement mediated lysis in the absence of analyte. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analyte-containing sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab' antibody fragments for coupling to lipid vesicles.

    摘要翻译: 本发明提供了用于检测流体样品中分析物的补体介导的用于标记包封的脂质囊泡(脂质体)的裂解的均相免疫测定系统。 这些系统不需要分离未结合的抗原和/或抗体缀合物,同时为分析物检测提供高度敏感的程序。 含有标记物的脂质体以使得脂质体具有免疫特异性的方式与抗体片段偶联,而避免在不存在分析物的情况下使脂质体对致敏介导的裂解进行敏化。 将抗体致敏脂质体(第一试剂)与含分析物的样品和任选的“虚拟”脂质体(其不包含包封标记物),第二抗体(第二试剂),最后用补体来源例如血浆 。 补体由脂质体 - 抗体 - 抗原 - 第二抗体复合物激活,引起脂质体溶解和伴随释放标记物。 还提供了在能够形成可逆凝胶的多糖存在下制备抗体致敏脂质体的方法以及用于制备用于偶联至脂质囊泡的衍生化Fab'抗体片段的方法。