摘要:
A protein having amino acid sequence in SEQ ID No.:1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID No.:1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译:衍生自人MP52的序列表的SEQ ID No.1中具有氨基酸序列的蛋白质及其二聚体蛋白质。 上述同型二聚体蛋白可以通过在N末端构建含有序列表SEQ ID No.1的DNA编码氨基酸序列的质粒与甲硫氨酸,将质粒导入大肠杆菌进行转化,溶解包合物 通过培养转化体获得的物质,从溶解的溶液中纯化单体蛋白质,将单体蛋白质重折叠成二聚体蛋白质并进行纯化。 上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。
摘要:
A protein having amino acid sequence in SEQ ID NO: 1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID NO: 1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译:衍生自人MP52的序列表的SEQ ID NO:1中具有氨基酸序列的蛋白质及其二聚体蛋白质。 可以通过在N末端构建含有序列表的SEQ ID NO:1的DNA编码氨基酸序列的质粒与甲硫氨酸在N末端引入,将质粒导入大肠杆菌进行转化,从而增溶包涵体 通过培养转化体获得,从溶解的溶液中纯化单体蛋白质,将单体蛋白重折叠成二聚体蛋白质并进行纯化。 上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。
摘要:
A protein consisting of the amino acid sequence in SEQ ID No: 1 derived from human MP52 and a homodimer protein thereof useful for treating cartilage and/or bone diseases and a process for its preparation.
摘要翻译:由源自人MP52的SEQ ID No:1的氨基酸序列和用于治疗软骨和/或骨疾病的同二聚体蛋白质组成的蛋白质及其制备方法。
摘要:
Disclosed are sugar free confectionery products, methods of making, and multilayer structures prepared from the confectionery products and chewing gum.
摘要:
Disclosed herein are polyol particles treated to modify the properties of the polyol. Polyol particles are coated with a crunch material coating composition to provide a particulate crunch material. Alternatively, hygroscopic polyol particles are coated with an inorganic material to provide a polyol having a reduced capacity for moisture absorption. Also disclosed herein is the incorporation of such pretreated polyol particle compositions in chewing gum.
摘要:
Disclosed are sugar free confectionery products, methods of making, and multilayer structures prepared from the confectionery products and chewing gum.
摘要:
Disclosed are sugar free confectionery products, methods of making, and multilayer structures prepared from the confectionery products and chewing gum.
摘要:
Disclosed are sugar free confectionery products, methods of making, and multilayer structures prepared from the confectionery products and chewing gum.
摘要:
Disclosed are sugar free confectionery products, methods of making, and multilayer structures prepared from the confectionery products and chewing gum.
摘要:
Disclosed are sugar free confectionery products, methods of making, and multilayer structures prepared from the confectionery products and chewing gum.