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公开(公告)号:US20190300961A1
公开(公告)日:2019-10-03
申请号:US15944725
申请日:2018-04-03
申请人: GUIZHOU MEDICAL UNIVERSITY , MATERNAL AND CHILD HEALTH HOSPITAL OF GUIYANG CITY , GUIZHOU ENTRY-EXIT INSPECTION AND QUARANTINE BUREAU
发明人: WENFENG YU , SHI ZHOU , ZHI HUANG , BI WANG , RUI GAO , DAI WEI ZHAO , HAI ZHUANG , YA LING LIANG
IPC分类号: C12Q1/6886 , C12Q1/6869 , C12Q1/686 , C07D227/02
摘要: A kit for determining ctDNA concentration and a method for determining ctDNA concentration is disclosed, wherein the method comprising dissolving extracted ctDNA in Tris-HCl buffer with pH>7.0 and adding into the buffer 2,7-bis(1-methyl-4-vinylpyridine)-9-ethylcarbazole iodised salt as a molecular probe, calculating the concentration of ctDNA in the solution by measuring the Fluorescence intensity of the solution. It has extremely high sensitivity in the determination of 0 μg/ml˜50 μg/ml ctDNA solution.