摘要:
This invention provides a system for performing PCR, and real time PCR in particular, with great speed and specificity. The system employs a heat block containing a liquid composition to rapidly transfer heat to and from reaction vessels. The system makes use of the reflective properties of the liquid metal to reflect signal from the PCR into the vessel and out the top. In this way, the signal can be measured by an optical assembly in real time without removing the vessels from the heat block.
摘要:
This invention provides an apparatus for providing heating and cooling of samples in sample vessels having uniform temperature profiles. The apparatus can be used for performing PCR, and real time PCR in particular, with control and uniformity. The system employs a heat block containing a liquid composition to efficiently transfer heat to and from reaction vessels.
摘要:
Methods for fabrication of microfluidic systems on printed circuit boards (PCB) are described. The PCB contains layers of insulating material and a layer or layers of metal buried within layers of insulating material. The metal layers are etched away, leaving fully enclosed microfluidic channels buried within the layers of insulating material.
摘要:
A printed circuit structure containing a fluidic chamber configured to receive an aqueous solution containing a sample to be analyzed and fluorophore for polymerase chain reaction analysis. The printed circuit structure also contains a heating element that provides for temperature cycling of the fluidic chamber to support polymerase chain reaction analysis.