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13 条记录 (耗时 0.025 秒)

    Method for directed packaging of molecular substances in protein shells
    2.
    发明授权
    Method for directed packaging of molecular substances in protein shells 失效
    蛋白质壳中分子物质定向包装的方法

    公开(公告)号：US07091028B1

    公开(公告)日：2006-08-15

    申请号：US10129207

    申请日：2000-11-03

    申请人： Gerald Boehm ,  Dirk Esser ,  Ulrich Schmidt

    发明人： Gerald Boehm ,  Dirk Esser ,  Ulrich Schmidt

    IPC分类号： C12N7/02

    CPC分类号： A61K9/5184 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/62 ,  C12N2710/22022 ,  C12N2710/22023 ,  C12N2810/50 ,  Y02A50/467

    摘要： The invention relates to a method for packaging molecular substances in protein shells, comprising the following steps: binding a protein shell fragment, via a first region to a matrix; bringing the protein shell fragment bound to the matrix into contact with the molecular substance, in order to bind the molecular substance to a second region of the protein shell fragment; separating the protein shell fragment with the bonded molecular substance, or the part thereof which contains the bonded molecular substance, from the matrix and assembling the separated protein shell fragments, or the part thereof which contains the bonded molecular substance, with other protein shell fragments to form a protein shell, whereby the separation and assembly can be carried out in any order.

    摘要翻译： 本发明涉及一种在蛋白质壳中包装分子物质的方法，包括以下步骤：将蛋白质壳片段经由第一区域与基质结合; 将与基质结合的蛋白质壳片段与分子物质接触，以便将分子物质结合到蛋白质壳片段的第二区域; 将含有结合分子物质的结合分子物质或其部分的蛋白质壳片段与基质分离，并将分离的蛋白质壳片段或其包含结合分子物质的部分与其它蛋白质壳片段组装到 形成蛋白质壳，由此分离和组装可以以任何顺序进行。

    METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS
    3.
    发明申请
    METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS 失效
    用衍生核酸结合蛋白转移分子物质的方法

    公开(公告)号：US20090155912A1

    公开(公告)日：2009-06-18

    申请号：US12207467

    申请日：2008-09-09

    申请人： Gerald Boehm ,  Dirk Esser

    发明人： Gerald Boehm ,  Dirk Esser

    IPC分类号： C12N15/74

    CPC分类号： C07K19/00 ,  C07K14/005 ,  C12N15/87 ,  C12N2710/10022 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2740/16022

    摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.

    摘要翻译： 本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。 原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。 当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。 原核蛋白凝结并压制核酸。 所述核酸可以在合适的温育后在靶细胞中吸收。

    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins
    4.
    发明授权
    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins 失效
    用原核核酸结合蛋白转移分子物质的方法

    公开(公告)号：US07435595B2

    公开(公告)日：2008-10-14

    申请号：US10954549

    申请日：2004-09-29

    申请人： Gerald Boehm ,  Dirk Esser

    发明人： Gerald Boehm ,  Dirk Esser

    IPC分类号： C12N15/00 ,  C12N15/88

    CPC分类号： C07K19/00 ,  C07K14/005 ,  C12N15/87 ,  C12N2710/10022 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2740/16022

    摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.

    摘要翻译： 本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。 原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。 当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。 原核蛋白凝结并压制核酸。 所述核酸可以在合适的温育后在靶细胞中吸收。

    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins
    5.
    发明授权
    Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins 失效
    用原核核酸结合蛋白转移分子物质的方法

    公开(公告)号：US07981676B2

    公开(公告)日：2011-07-19

    申请号：US12207467

    申请日：2008-09-09

    申请人： Gerald Boehm ,  Dirk Esser

    发明人： Gerald Boehm ,  Dirk Esser

    IPC分类号： C12N15/00

    CPC分类号： C07K19/00 ,  C07K14/005 ,  C12N15/87 ,  C12N2710/10022 ,  C12N2710/16222 ,  C12N2710/16622 ,  C12N2740/16022

    摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.

    摘要翻译： 本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。 原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。 当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。 原核蛋白凝结并压制核酸。 所述核酸可以在合适的温育后在靶细胞中吸收。

    Method for characterizing and separating molecular associates
    9.
    发明授权
    Method for characterizing and separating molecular associates 失效
    表征和分离分子缔合物的方法

    公开(公告)号：US07488605B2

    公开(公告)日：2009-02-10

    申请号：US10950347

    申请日：2004-09-23

    申请人： Gerald Boehm ,  Ulrich Schmidt

    发明人： Gerald Boehm ,  Ulrich Schmidt

    IPC分类号： G01N33/566

    CPC分类号： G01N33/56983 ,  G01N33/537 ,  G01N33/6803 ,  G01N33/6896 ,  G01N2333/4709

    摘要： The present invention relates to a method for characterising and optionally separating molecular associates, especially particles having a size smaller than 300 nm. Partial units of the molecular associates are used as markers, whereby said units are marked with fluorescent dyes. The marked associates and aggregates are characterised by means of a FACS (fluorescence-activated cell sorter).

    摘要翻译： 本发明涉及用于表征和任选分离分子缔合体的方法，特别是尺寸小于300nm的颗粒。 分子缔合物的部分单元用作标记，其中所述单元用荧光染料标记。 标记的缔合物和聚集体通过FACS（荧光激活细胞分选仪）的特征。