公开(公告)号：US06225106B1

公开(公告)日：2001-05-01

申请号：US09479409

申请日：2000-01-06

申请人： Gijsbert Gerritse ,  Wilhelmus J. Quax

发明人： Gijsbert Gerritse ,  Wilhelmus J. Quax

IPC分类号： C12N1578

CPC分类号： C07K14/21 ,  C12N9/20 ,  C12N15/78

摘要： A new expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

摘要翻译： 提供了一种新的表达系统，其包含脂肪酶调节级联的组分。 如本文所公开的脂肪酶调节级联包括激酶，DNA结合调节剂，聚合酶，启动子，上游激活序列和分泌因子。 还提供质粒和转化细胞以及使用质粒转化宿主细胞的方法。 此外，提供了可以调节蛋白质的表达的激酶，可调节蛋白质表达的DNA结合调节剂，产碱假单胞菌聚合酶，产碱假马杆菌54启动子，产碱假单胞菌（Pseudomonas alcaligenes）上游激活序列，假单胞菌 产碱分泌因子XcpP，XcpQ，XcpR，XcpS，XcpT，XcpU，XcpV，XcpW，XcpX，XcpY，XcpZ和xcp调节剂OrfV OrfX。

公开(公告)号：US06313283B1

公开(公告)日：2001-11-06

申请号：US09479453

申请日：2000-01-06

申请人： Gijsbert Gerritse ,  Wilhelmus J. Quax

发明人： Gijsbert Gerritse ,  Wilhelmus J. Quax

IPC分类号： C12N1531

CPC分类号： C07K14/21 ,  C12N9/20 ,  C12N15/78

摘要： A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

摘要翻译： 提供了一种新的表达系统，其包含脂肪酶调节级联的组分。 如本文所公开的脂肪酶调节级联包括激酶，DNA结合调节剂，聚合酶，启动子，上游激活序列和分泌因子。 还提供质粒和转化细胞以及使用质粒转化宿主细胞的方法。 此外，提供了可以调节蛋白质的表达的激酶，可调节蛋白质表达的DNA结合调节剂，产碱假单胞菌聚合酶，产碱假马杆菌54启动子，产碱假单胞菌（Pseudomonas alcaligenes）上游激活序列，假单胞菌 产碱分泌因子XcpP，XcpQ，XcpR，XcpS，XcpT，XcpU，XcpV，XcpW，XcpX，XcpY，XcpZ和xcp调节剂OrfV OrfX。

公开(公告)号：US6048710A

公开(公告)日：2000-04-11

申请号：US911853

申请日：1997-08-15

申请人： Gijsbert Gerritse ,  Wilhelmus J. Quax

发明人： Gijsbert Gerritse ,  Wilhelmus J. Quax

IPC分类号： C07K14/21 ,  C12N9/12 ,  C12N9/20 ,  C12N15/00 ,  C12N15/31 ,  C12N15/54 ,  C12N15/78 ,  C12P21/00 ,  C12N9/00 ,  C12N15/63

CPC分类号： C07K14/21 ,  C12N15/78 ,  C12N9/20

摘要： A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

摘要翻译： 提供了一种新的表达系统，其包含脂肪酶调节级联的组分。 如本文所公开的脂肪酶调节级联包括激酶，DNA结合调节剂，聚合酶，启动子，上游激活序列和分泌因子。 还提供质粒和转化细胞以及使用质粒转化宿主细胞的方法。 此外，提供了可以调节蛋白质的表达的激酶，可调节蛋白质表达的DNA结合调节剂，产碱假单胞菌聚合酶，产碱假马杆菌54启动子，产碱假单胞菌（Pseudomonas alcaligenes）上游激活序列，假单胞菌 产碱分泌因子XcpP，XcpQ，XcpR，XcpS，XcpT，XcpU，XcpV，XcpW，XcpX，XcpY，XcpZ和xcp调节剂OrfV OrfX。

公开(公告)号：US5246838A

公开(公告)日：1993-09-21

申请号：US662005

申请日：1991-02-28

申请人： Jan M. Van Dijl ,  Hilde E. Smith ,  Sierd Bron ,  Wilhelmus J. Quax ,  Gerard Venema

发明人： Jan M. Van Dijl ,  Hilde E. Smith ,  Sierd Bron ,  Wilhelmus J. Quax ,  Gerard Venema

IPC分类号： C12N1/21 ,  C12N9/28 ,  C12N9/52 ,  C12N9/56 ,  C12N9/86 ,  C12N15/09 ,  C12N15/57 ,  C12N15/62 ,  C12N15/67 ,  C12N15/70 ,  C12N15/75 ,  C12R1/125 ,  C12R1/19 ,  C12R1/42

CPC分类号： C12N9/2417 ,  C12N15/625 ,  C12N15/67 ,  C12N15/70 ,  C12N15/75 ,  C12N9/52 ,  C12N9/86 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/61

摘要： The subject invention describes the cloning and overexpression of leader peptidase genes. A method for isolating a leader peptidase gene is disclosed. Overexpression of the signal peptidase in a suitable host species leads to an enhanced rate of protein processing.

公开(公告)号：US5037751A

公开(公告)日：1991-08-06

申请号：US405553

申请日：1989-09-11

申请人： Mauro A. Bertola ,  Arthur F. Marx ,  Hein S. Koger ,  Wilhelmus J. Quax ,  Cornelis J. van der Laken ,  Gareth T. Phillips ,  Brian W. Robertson ,  Peter D. Watts

发明人： Mauro A. Bertola ,  Arthur F. Marx ,  Hein S. Koger ,  Wilhelmus J. Quax ,  Cornelis J. van der Laken ,  Gareth T. Phillips ,  Brian W. Robertson ,  Peter D. Watts

IPC分类号： C12N15/09 ,  A61K31/19 ,  A61K31/33 ,  A61P25/04 ,  A61P29/00 ,  C07C57/30 ,  C07D209/46 ,  C07H21/04 ,  C12N1/20 ,  C12N1/21 ,  C12N9/18 ,  C12N15/00 ,  C12P7/40 ,  C12P17/00 ,  C12P41/00 ,  C12R1/06 ,  C12R1/10 ,  C12R1/125 ,  C12R1/19 ,  C12R1/38 ,  C12R1/385 ,  C12R1/39 ,  C12R1/40 ,  C12R1/465 ,  C12R1/785

CPC分类号： C12P41/005 ,  C12N9/18 ,  C12P17/00 ,  C12P7/40 ,  Y10S435/822 ,  Y10S435/83 ,  Y10S435/836 ,  Y10S435/839 ,  Y10S435/849 ,  Y10S435/874 ,  Y10S435/886

摘要： A process for the preparation of a pharmaceutically active compound in a stereospecific form of the formula ##STR1## or a pharmaceutically acceptable salt or ester thereof, like an alkali metal salt or an alkaline earth metal salt or a pivaloyl ester, wherein R.sub.1 represents an optionally substituted aryl group such as a phenyl or naphthyl group optionally included in a heterocyclic ring system, which is optionally substituted, or represents a heteroaromatic ring system containing in addition to carbon atoms one or more atoms selected from nitrogen, sulphur and oxygen, this ring system being optionally substituted, which comprises subjecting a compound of the formula ##STR2## wherein R.sub.2 is an ester residue and preferably an alkyl group optionally substituted, to the action of a micro-organism having the ability for stereoselective hydrolysis of compound (II) into compound (I), having at least 80% by weight the S-configuration, and if desired converting compound (I) into the pharmaceutically acceptable salt or ester thereof.

公开(公告)号：US06630328B2

公开(公告)日：2003-10-07

申请号：US09899482

申请日：2001-07-05

申请人： Wilhelmus J. Quax

发明人： Wilhelmus J. Quax

IPC分类号： C12P2104

CPC分类号： C07K14/32 ,  C07K2319/00 ,  C12N15/75 ,  C12P21/02

摘要： The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nucleic acid amino acid sequences for the Bacillus subtilis secretion factors SecDF. The present invention also provides improved methods for the secretion of heterologous or homologous proteins in gram-positive microorganisms.

摘要翻译： 本发明涉及革兰氏阳性微生物中的分泌。 本发明提供枯草芽孢杆菌分泌因子SecDF的核酸氨基酸序列。 本发明还提供了用于在革兰氏阳性微生物中分泌异源或同源蛋白质的改进方法。

公开(公告)号：US6033823A

公开(公告)日：2000-03-07

申请号：US285957

申请日：1999-04-05

申请人： Jan M. Van Der Laan ,  Adriana M. Riemens ,  Wilhelmus J. Quax

发明人： Jan M. Van Der Laan ,  Adriana M. Riemens ,  Wilhelmus J. Quax

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/84 ,  C12N15/00 ,  C12N15/55 ,  C12P35/02 ,  C12P37/06 ,  C12R1/05 ,  C12R1/745 ,  C12R1/80 ,  C12N15/11 ,  C12N15/70

CPC分类号： C12P35/02 ,  C12N9/84 ,  C12P37/06

摘要： New mutant .beta.-lactam Penicillin G acylases are provided, exhibiting altered substrate specificities. These Penicillin G acylases are obtained by expression of a gene encoding said Penicillin G acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type Penicillin G acylase.

摘要翻译： 提供新的突变体β-内酰胺青霉素G酰基转移酶，显示改变的底物特异性。 这些青霉素G酰基转移酶是通过表达编码所述青霉素G酰基转移酶的基因并具有至少在一个氨基酸中与野生型青霉素G酰基转移酶不同的氨基酸序列获得的。

公开(公告)号：US5364782A

公开(公告)日：1994-11-15

申请号：US623953

申请日：1990-11-29

申请人： Wilhelmus J. Quax ,  Yves Laroche ,  Adrianus W. H. Vollebregt ,  Patrick Stanssens ,  Marc Lauwereys

发明人： Wilhelmus J. Quax ,  Yves Laroche ,  Adrianus W. H. Vollebregt ,  Patrick Stanssens ,  Marc Lauwereys

IPC分类号： C12P19/14 ,  C12N20060101 ,  C12N1/21 ,  C12N9/26 ,  C12N9/28 ,  C12N15/09 ,  C12N15/56 ,  C12N15/70 ,  C12N15/75 ,  C12R1/07 ,  C12R1/10 ,  C12R1/125 ,  C12R1/19 ,  C12S3/12 ,  D06L1/14 ,  D06M16/00

CPC分类号： C12N15/75 ,  C12N15/70 ,  C12N9/2414 ,  C12N9/2417

摘要： Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.

摘要翻译： PCT No.PCT / EP90 / 01042 Sec。 371 1990年12月2日第 102（e）1990年12月2日日期PCT提交1990年6月27日PCT公布。 公开号WO91 / 00353 日期：1991年1月10日。作为从微生物分离的遗传工程化的α-淀粉酶基因的表达产物，优选和酸稳定的α-淀粉酶是优选属于杆菌类的表达产物。 化学和酶诱变方法都是例如。 亚硫酸氢盐法和间接异源双链DNA上的酶学错配。 突变体α-淀粉酶具有优异的性质，例如 在广泛的pH范围内提高热稳定性，用于淀粉加工和纺织品退浆中的工业应用。

公开(公告)号：US06521421B1

公开(公告)日：2003-02-18

申请号：US09462842

申请日：2000-03-22

申请人： Wilhelmus J. Quax ,  Robert M. Caldwell

发明人： Wilhelmus J. Quax ,  Robert M. Caldwell

IPC分类号： C12P2106

CPC分类号： C12N9/90 ,  C12Y503/04001

摘要： The present invention relates to nucleic acid sequences and amino acid sequence for Bacillus subtills disulfide bond isomerases, Dsb1 and Dsb2 and methods for increasing the secretion of heterologous and homologous proteins in gram-positive microorganisms.

摘要翻译： 本发明涉及用于枯草芽孢杆菌二硫键异构酶，Dsb1和Dsb2的核酸序列和氨基酸序列以及增加革兰氏阳性微生物中异源和同源蛋白质分泌的方法。

公开(公告)号：US5457032A

公开(公告)日：1995-10-10

申请号：US731157

申请日：1991-05-09

申请人： Wilhelmus J. Quax ,  Onno Misset ,  Jan M. Van Der Laan ,  Herman B. M. Lenting

发明人： Wilhelmus J. Quax ,  Onno Misset ,  Jan M. Van Der Laan ,  Herman B. M. Lenting

IPC分类号： C12N1/15 ,  C12N1/21 ,  C12N9/84 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12R1/05 ,  C12R1/19 ,  C12R1/38 ,  C12P37/00

CPC分类号： C12N9/84 ,  C12Y305/01011

摘要： New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.

摘要翻译： 提供了新的突变体β-内酰基酰基转移酶，其表现出改变的底物特异性。 这些β-内酰胺酰基转移酶通过表达编码所述β-内酰胺酰基转移酶的基因并具有至少在一个氨基酸中与野生型β-内酰胺酰基转移酶不同的氨基酸序列获得。