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公开(公告)号：US20100273659A1
公开(公告)日：2010-10-28
申请号：US12529340
申请日：2008-02-29
申请人： Grace DeSantis , Jennifer Chaplin , Ellen Chi , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden , Dan Robertson
发明人： Grace DeSantis , Jennifer Chaplin , Ellen Chi , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden , Dan Robertson
IPC分类号： C40B20/00 , C12N15/55 , C12N15/63 , C12N9/78 , C12P7/40 , C12P7/42 , C12P13/00 , C12P7/54 , C12P7/56 , C12P19/34 , C12Q1/68 , C12P1/00 , C12Q1/34
CPC分类号： C12P7/42 , C12N9/78 , C12P13/002 , C12P13/004 , C12P13/005 , C12P13/04 , C12P41/007
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
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公开(公告)号：US08334125B2
公开(公告)日：2012-12-18
申请号：US13169853
申请日：2011-06-27
申请人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden, legal representative , Mark Burk , Dan Robertson
发明人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Mark Burk , Dan Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C12Q1/68 , A61K38/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US07993901B2
公开(公告)日：2011-08-09
申请号：US12629818
申请日：2009-12-02
申请人： Grace Desantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Marcy Madden, legal representative , Mark Burk , Dan Robertson
发明人： Grace Desantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Mark Burk , Dan Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C12Q1/68 , A61K38/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US20090176976A1
公开(公告)日：2009-07-09
申请号：US12397204
申请日：2009-03-03
申请人： Grace DESANTIS , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson , Darcy Madden
发明人： Grace DESANTIS , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson , Darcy Madden
IPC分类号： C12N15/11
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US09217164B2
公开(公告)日：2015-12-22
申请号：US12529340
申请日：2008-02-29
申请人： Jennifer A. Chaplin , Ellen Chi , Grace DeSantis , Mark Madden , Darcy Madden , Aileen Milan , Dan E. Robertson , Jay M. Short , David P. Weiner
发明人： Jennifer A. Chaplin , Ellen Chi , Grace DeSantis , Mark Madden , Aileen Milan , Dan E. Robertson , Jay M. Short , David P. Weiner
CPC分类号： C12P7/42 , C12N9/78 , C12P13/002 , C12P13/004 , C12P13/005 , C12P13/04 , C12P41/007
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
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公开(公告)号：US07651849B2
公开(公告)日：2010-01-26
申请号：US12397204
申请日：2009-03-03
申请人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay M. Short , David Paul Weiner , Mark Madden , Darcy Madden, legal representative , Mark J. Burk , Dan E. Robertson
发明人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay M. Short , David Paul Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , A61K38/00 , C07H21/04 , C12Q1/68 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US07521216B2
公开(公告)日：2009-04-21
申请号：US10146772
申请日：2002-05-15
申请人： Grace DeSantis , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Darcy Madden, legal representative , Mark J. Burk , Dan E. Robertson
发明人： Mark Madden
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , A61K38/00 , C07H21/04 , C12Q1/68 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US20120009633A1
公开(公告)日：2012-01-12
申请号：US13238227
申请日：2011-09-21
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶及其制备和使用方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US08034595B2
公开(公告)日：2011-10-11
申请号：US11868909
申请日：2007-10-08
申请人： Mark Madden , David Weiner , Jennifer Ann Chaplin
发明人： Mark Madden , David Weiner , Jennifer Ann Chaplin
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶及其制备和使用方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US08501451B2
公开(公告)日：2013-08-06
申请号：US13238227
申请日：2011-09-21
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶和制备和使用它们的方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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