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    Bone mineralization proteins, DNA, vectors, expression systems
    1.
    发明授权
    Bone mineralization proteins, DNA, vectors, expression systems 失效
    骨矿化蛋白，DNA，载体，表达系统

    公开(公告)号：US06300127B1

    公开(公告)日：2001-10-09

    申请号：US09124238

    申请日：1998-07-29

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C12N500

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。

    LIM mineralization protein splice variants
    2.
    发明授权
    LIM mineralization protein splice variants 有权

    公开(公告)号：US07045614B1

    公开(公告)日：2006-05-16

    申请号：US09959578

    申请日：2000-04-28

    申请人： Scott D. Boden ,  Gregory A. Hair

    发明人： Scott D. Boden ,  Gregory A. Hair

    IPC分类号： C07H21/04

    CPC分类号： C07K14/51 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4702 ,  C07K14/52

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising splice variants of nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding splice variants of LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    Bone mineralization proteins, DNA, vectors, expression systems
    3.
    发明授权
    Bone mineralization proteins, DNA, vectors, expression systems 有权
    骨矿化蛋白，DNA，载体，表达系统

    公开(公告)号：US06521750B2

    公开(公告)日：2003-02-18

    申请号：US09986621

    申请日：2001-11-09

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C07H2100

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。

    Bone mineralization proteins, DNA, vectors, expression systems
    4.
    发明授权
    Bone mineralization proteins, DNA, vectors, expression systems 有权
    骨矿化蛋白，DNA，载体，表达系统

    公开(公告)号：US07825228B2

    公开(公告)日：2010-11-02

    申请号：US10951236

    申请日：2004-09-27

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12N15/00 ,  G01N33/53

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。

    Bone mineralization proteins, DNA, vectors, expression systems
    5.
    发明授权
    Bone mineralization proteins, DNA, vectors, expression systems 有权
    骨矿化蛋白，DNA，载体，表达系统

    公开(公告)号：US06444803B1

    公开(公告)日：2002-09-03

    申请号：US09721975

    申请日：2000-11-27

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C07H2104

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected a osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过将成骨前体细胞与具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染来融合脊柱的方法，将转染的成骨前体细胞与基质混合并将基质与 脊柱。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。

    Novel Bone Mineralization Proteins, DNA, Vectors, Expression Systems
    6.
    发明申请
    Novel Bone Mineralization Proteins, DNA, Vectors, Expression Systems 审中-公开
    新型骨矿化蛋白，DNA，载体，表达系统

    公开(公告)号：US20090247733A1

    公开(公告)日：2009-10-01

    申请号：US11857938

    申请日：2007-09-19

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C07K14/47

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。

    Bone mineralization proteins, DNA, vectors expression systems
    7.
    发明授权
    Bone mineralization proteins, DNA, vectors expression systems 有权
    骨矿化蛋白，DNA，载体表达系统

    公开(公告)号：US06858431B2

    公开(公告)日：2005-02-22

    申请号：US09986625

    申请日：2001-11-09

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C12N15/09 ,  A61K31/711 ,  A61K38/00 ,  A61K48/00 ,  A61P19/00 ,  A61P43/00 ,  C07K14/47 ,  C07K16/18 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N15/12 ,  C12P21/08 ,  C12Q1/68 ,  C12N15/63 ,  C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12N15/86 ,  C12N15/87

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。