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公开(公告)号：US20160340663A1
公开(公告)日：2016-11-24
申请号：US15156759
申请日：2016-05-17
发明人： Migiwa SUDA , Jiro OKUMA , Asuka YAMAGUCHI , Yoshitsugu HIROSE , Yasuhiro KONDO , Masaru SATO , Daisuke SHIBATA
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/12 , C12P19/14 , C12Y302/01004
摘要： A thermostable glycoside hydrolase having a glycoside hydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (D) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 51 to 485 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA at least under conditions of 65° C. and pH 4.0, and a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; or (E) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from position 51 to position 485 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of PSA at least under conditions of 65° C. and pH 4.0, and a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0.
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公开(公告)号：US20160053246A1
公开(公告)日：2016-02-25
申请号：US14759740
申请日：2014-03-27
发明人： Migiwa SUDA , Jiro OHKUMA , Asuka YAMAGUCHI , Yoshitsugu HIROSE , Yasuhiro KONDO , Tomohiko KATO , Daisuke SHIBATA
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： The thermostable cellobiohydrolase of the present invention is a polypeptide which has cellobiohydrolase activity at least under conditions of a temperature of 75° C. and a pH of 5.5, and which includes a polypeptide including an amino acid sequence represented by SEQ ID NO: 1, 3, 5, or 7, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in an amino acid sequence represented by SEQ ID NO: 1, 3, 5, or 7, or a polypeptide including an amino acid sequence having 80% or greater but less than 100% sequence identity with an amino acid sequence represented by SEQ ID NO: 1, 3, 5, or 7.
摘要翻译： 本发明的热稳定纤维二糖水解酶是至少在温度75℃，pH5.5的条件下具有纤维二糖水解酶活性的多肽，其含有包含SEQ ID NO：1所示的氨基酸序列的多肽， 3，5或7所述的多肽，所述多肽包括氨基酸序列，其中一个或几个氨基酸被缺失，取代或添加在由SEQ ID NO：1,3,5或7表示的氨基酸序列中，或 多肽，其包含与SEQ ID NO：1,3,5或7所示的氨基酸序列具有80％以上但小于100％的序列同一性的氨基酸序列。
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公开(公告)号：US20160032265A1
公开(公告)日：2016-02-04
申请号：US14813894
申请日：2015-07-30
发明人： Yasuhiro KONDO , Yoshitsugu HIROSE , Asuka YAMAGUCHI , Migiwa SUDA , Jiro OKUMA , Tomohiko KATO , Daisuke SHIBATA
CPC分类号： C12N9/248 , C07K2319/00 , C07K2319/02 , C12N9/2445 , C12Y302/01037
摘要： A thermostable β-xylosidase including a β-xylosidase catalytic domain, the β-xylosidase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-xylopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 6.0; or (C) a polypeptide including an amino acid sequence having at least 80% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-xylopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 6.0.
摘要翻译： 包含α-脱氧核糖酶催化结构域的热稳定性 - 木糖苷酶，所述β-氧化酶催化结构域包括：（A）包含由SEQ ID NO：1表示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸缺失，取代或添加在SEQ ID NO：1所示的氨基酸序列中，并且具有使用对硝基苯基-β 至少在温度为85℃，pH为6.0的条件下作为底物， 或（C）包含与SEQ ID NO：1所示的氨基酸序列具有至少80％序列同一性的氨基酸序列的多肽，并且使用对硝基苯基-β-吡喃木糖作为底物具有水解活性 至少在温度为85℃，pH为6.0的条件下。
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公开(公告)号：US20170275606A1
公开(公告)日：2017-09-28
申请号：US15467542
申请日：2017-03-23
发明人： Jiro OKUMA , Yoshitsugu HIROSE , Migiwa SUDA , Asuka YAMAGUCHI , Yasuhiro KONDO , Masaru SATO , Daisuke SHIBATA
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/12 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： A thermostable cellobiohydrolase, having a cellulose-binding motif domain including (A1) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1, (B1) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, or (C1) a polypeptide including an amino acid sequence having 70% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, and also having a cellobiohydrolase catalytic domain, wherein the thermostable cellobiohydrolase exhibits hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 95° C. and pH 5.5.
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公开(公告)号：US20160122791A1
公开(公告)日：2016-05-05
申请号：US14816603
申请日：2015-08-03
发明人： Asuka YAMAGUCHI , Jiro OKUMA , Yoshitsugu HIROSE , Migiwa SUDA , Yasuhiro KONDO , Tomohiko KATO , Daisuke SHIBATA
CPC分类号： C12P19/14 , C12N9/2437 , C12P19/02 , C12Y302/01004
摘要： A hyperthermostable endoglucanase including an endoglucanase catalytic domain, the endoglucanase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-cellobioside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5; or (C) a polypeptide including an amino acid sequence having at least 70% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-cellobioside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5.
摘要翻译： 包含内切葡聚糖酶催化结构域的超热稳定内切葡聚糖酶，所述内切葡聚糖酶催化结构域包括：（A）包含由SEQ ID NO：1表示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸缺失，取代或添加在SEQ ID NO：1所示的氨基酸序列中，并且具有使用对硝基苯基-β 至少在温度为100℃，pH为5.5的条件下，作为底物的纤维二糖苷; 或（C）多肽，其包含与SEQ ID NO：1所示的氨基酸序列具有至少70％序列同一性的氨基酸序列，并且使用对硝基苯基-β-D-纤维二糖苷作为底物具有水解活性 至少在温度为100℃，pH为5.5的条件下。
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公开(公告)号：US20160076017A1
公开(公告)日：2016-03-17
申请号：US14854562
申请日：2015-09-15
发明人： Yasuhiro KONDO , Jiro OKUMA , Yoshitsugu HIROSE , Asuka YAMAGUCHI , Migiwa SUDA , Tomohiko KATO , Daisuke SHIBATA
CPC分类号： C12N9/2477 , C07K14/435 , C12N9/2445 , C12N9/2485 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01037
摘要： A thermostable β-xylosidase, having a β-xylosidase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0.
摘要翻译： 一种具有β-脱氧核苷酸酶催化结构域的热稳定性 - 木糖苷酶，包括：（A）包含SEQ ID NO：1,3或5所示氨基酸序列的多肽，（B）包含氨基酸序列的多肽 其中至少一个氨基酸已被缺失，取代或添加在SEQ ID NO：1,3或5所示的氨基酸序列中，并且具有对对硝基苯基-β-吡喃木糖苷底物的水解活性 至少在80℃和pH4.0的条件下，或（C）包含与SEQ ID NO：1,3或5所示氨基酸序列具有80％或更高的氨基酸序列的氨基酸序列的多肽，并具有水解 至少在80℃和pH4.0的条件下，对对硝基苯基-β-D-吡喃木糖苷的底物的活性。
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公开(公告)号：US20150017692A1
公开(公告)日：2015-01-15
申请号：US14324340
申请日：2014-07-07
发明人： Maiko TANAKA , Shigenobu MITSUZAWA , Satoru SHINKAWA , Daisuke SHIBATA , Takeshi ARA , Migiwa TAKEDA
CPC分类号： C12N9/2445 , C12N9/2437 , C12N9/244 , C12N9/2482 , C12N9/2485 , C12P19/02 , C12P19/14 , C12Y302/01021 , D21C9/005
摘要： The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.
摘要翻译： 本发明涉及具有β-葡糖苷酶活性的多肽，其包含SEQ ID NO：1所示的氨基酸序列，包括其中一个或几个氨基酸被缺失，取代或 加入由SEQ ID NO：1表示的氨基酸序列，或包含与SEQ ID NO：1所示的氨基酸序列具有92％以上序列同一性的氨基酸序列的多肽。根据本发明， 衍生自解淀粉解淀粉的葡糖苷酶，编码葡萄糖苷酶的多核苷酸，用于表达葡糖苷酶的表达载体，与该表达载体结合的转化体，以及使用＆bgr生产纤维素降解产物的方法 ; - 葡糖苷酶。
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公开(公告)号：US20150017691A1
公开(公告)日：2015-01-15
申请号：US14324300
申请日：2014-07-07
发明人： Maiko TANAKA , Shigenobu MITSUZAWA , Satoru SHINKAWA , Daisuke SHIBATA , Takeshi ARA , Migiwa TAKEDA
CPC分类号： C12N9/2445 , C12N9/2437 , C12N9/248 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.
摘要翻译： 本发明涉及具有β-葡糖苷酶活性的多肽，其包含SEQ ID NO：1所示的氨基酸序列，包括其中一个或几个氨基酸被缺失，取代或 加入由SEQ ID NO：1表示的氨基酸序列，或包含与SEQ ID NO：1所示的氨基酸序列具有92％以上序列同一性的氨基酸序列的多肽。根据本发明， 衍生自解淀粉解淀粉的葡糖苷酶，编码葡萄糖苷酶的多核苷酸，用于表达葡糖苷酶的表达载体，与该表达载体结合的转化体，以及使用＆bgr生产纤维素降解产物的方法 ; - 葡糖苷酶。
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公开(公告)号：US20150017690A1
公开(公告)日：2015-01-15
申请号：US14324281
申请日：2014-07-07
发明人： Maiko TANAKA , Shigenobu MITSUZAWA , Satoru SHINKAWA , Daisuke SHIBATA , Takeshi ARA , Migiwa TAKEDA
CPC分类号： C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021 , D21C5/005 , Y02P20/52
摘要： The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.
摘要翻译： 本发明涉及具有β-葡糖苷酶活性的多肽，其包含SEQ ID NO：1所示的氨基酸序列，包括其中一个或几个氨基酸被缺失，取代或 加入由SEQ ID NO：1表示的氨基酸序列，或包含与SEQ ID NO：1所示的氨基酸序列具有91％以上的氨基酸序列的氨基酸序列的多肽。根据本发明， 衍生自解淀粉解淀粉的葡萄糖苷酶，编码葡萄糖苷酶的多核苷酸，用于表达葡萄糖苷酶的表达载体，与该表达载体结合的转化体，以及使用＆bgr生产纤维素降解产物的方法 ; - 葡糖苷酶。
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公开(公告)号：US20160168553A1
公开(公告)日：2016-06-16
申请号：US14964216
申请日：2015-12-09
发明人： Jiro OKUMA , Migiwa SUDA , Asuka YAMAGUCHI , Yoshitsugu HIROSE , Yasuhiro KONDO , Masaru SATO , Daisuke SHIBATA
CPC分类号： C12N9/2445 , C12N9/2471 , C12N9/248 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01023 , C12Y302/01037 , Y02E50/16
摘要： A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1 or 2; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5; or (C) a polypeptide including an amino acid sequence having at least 80% sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5.
摘要翻译： 包含葡糖苷酶催化结构域的热稳定性 - 葡萄糖苷酶，所述β-葡糖苷酶催化结构域包括：（A）包含由SEQ ID NO：1或2表示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸已被缺失，取代或加入到由SEQ ID NO：1或2表示的氨基酸序列中，并且使用对硝基苯基-bgr具有水解活性 ; D-吡喃葡萄糖苷作为底物，至少在温度为100℃，pH为5.5的条件下; 或（C）包含与SEQ ID NO：1或2表示的氨基酸序列具有至少80％序列同一性的氨基酸序列的多肽，并且使用对硝基苯基-β-D-吡喃葡萄糖苷作为水解活性具有水解活性 至少在温度为100℃，pH为5.5的条件下进行。
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