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公开(公告)号：US20110256589A1
公开(公告)日：2011-10-20
申请号：US13069514
申请日：2011-03-23
申请人： Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
发明人： Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
CPC分类号： C12N9/1247 , C12P19/34 , C12Y207/07006
摘要： The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.
摘要翻译： 本发明通过引入导致酶的热稳定性的新突变来提供T7RNA聚合酶的改进变体。 根据本发明，位置Val426，Ser633，Val650，Thr654，Ala702，Val795及其组合的氨基酸取代是有利的。
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公开(公告)号：US09193959B2
公开(公告)日：2015-11-24
申请号：US13069514
申请日：2011-03-23
申请人： Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
发明人： Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
CPC分类号： C12N9/1247 , C12P19/34 , C12Y207/07006
摘要： The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.
摘要翻译： 本发明通过引入导致酶的热稳定性的新突变来提供T7RNA聚合酶的改进变体。 根据本发明，位置Val426，Ser633，Val650，Thr654，Ala702，Val795及其组合的氨基酸取代是有利的。
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3.发明申请公开(公告)号：US20120252071A1
公开(公告)日：2012-10-04
申请号：US13436110
申请日：2012-03-30
CPC分类号： C12N9/1247 , C12Y207/07006
摘要： The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.
摘要翻译： 本公开提供了T7RNA聚合酶的新变体。 根据本发明的T7变体的实施方案包括T7多肽的氨基酸序列的位置723上的半胱氨酸 - 丝氨酸取代。 根据本发明的T7变体的实施方案具有DNA依赖性RNA聚合酶酶活性，并且通过氧化硫醇基形成分子内同型二聚体的趋势降低。 本文公开的T7变体内的氨基酸取代最小程度地影响T7多肽的RNA聚合酶活性。 此外，所公开的实施方案的突变可以任选地与与野生型参考物相比提供增强的热稳定性的突变组合。
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4.发明授权Stabilized aqueous alpha-galactosidase composition and methods relating thereto 有权稳定的α-半乳糖苷水溶液组合物及其相关方法公开(公告)号：US08476053B2
公开(公告)日：2013-07-02
申请号：US12762465
申请日：2010-04-19
CPC分类号： C07K14/435
摘要： The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase.
摘要翻译： 本发明提供含有具有α-半乳糖苷酶的酶活性的蛋白质的含水组合物。 本发明还提供了一种稳定包含具有α-半乳糖苷酶的酶活性的蛋白质的水性组合物的方法，以及制备包含具有α-半乳糖苷酶的酶活性的蛋白质的纯化含水组合物的方法。
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5.发明申请STABILIZED AQUEOUS ALPHA-GALACTOSIDASE COMPOSITION AND METHODS RELATING THERETO 有权稳定的水性阿尔法胶质糖苷组合物及其相关方法公开(公告)号：US20100273235A1
公开(公告)日：2010-10-28
申请号：US12762465
申请日：2010-04-19
IPC分类号： C12N9/40
CPC分类号： C07K14/435
摘要： The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase.
摘要翻译： 本发明提供含有具有α-半乳糖苷酶的酶活性的蛋白质的含水组合物。 本发明还提供了一种稳定包含具有α-半乳糖苷酶的酶活性的蛋白质的水性组合物的方法，以及制备包含具有α-半乳糖苷酶的酶活性的蛋白质的纯化含水组合物的方法。
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6.发明授权Method for producing an active heterodimeric AMV-RT in prokaryotic cells 有权用于在原核细胞中产生活性异二聚体AMV-RT的方法公开(公告)号：US06902920B2
公开(公告)日：2005-06-07
申请号：US09960428
申请日：2001-09-21
申请人： Harald Sobek , Rainer Mueller , Manfred Schmidt , Bruno Frey , Bernhard Suppmann , Rainer Schmuck , Johann-Peter Thalhofer , Peter Pallua , Markus Pajatsch
发明人： Harald Sobek , Rainer Mueller , Manfred Schmidt , Bruno Frey , Bernhard Suppmann , Rainer Schmuck , Johann-Peter Thalhofer , Peter Pallua , Markus Pajatsch
CPC分类号： C12N9/1276
摘要： The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.
摘要翻译： 在原核细胞，特别是大肠杆菌（E.coli）中，来自禽成髓细胞病毒（AMV-RT）的逆转录酶的异源表达在本发明中描述。 本发明还包括简化异二聚体AMV-RT的纯化的某些措施。
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公开(公告)号：US20110250598A1
公开(公告)日：2011-10-13
申请号：US13042628
申请日：2011-03-08
CPC分类号： C12Q1/686 , C12N9/1252 , C12Q2527/137 , C12Q2521/101
摘要： The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step.
摘要翻译： 本发明涉及完全不含洗涤剂的热稳定性DNA聚合酶的制剂及其在实时聚合酶链式反应（PCR）中的特别用途。 如果所选择的纯化方法不需要在任何纯化步骤中添加洗涤剂，则可以获得这样的制剂。
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公开(公告)号：US20070154941A1
公开(公告)日：2007-07-05
申请号：US11712877
申请日：2007-03-01
CPC分类号： C12N9/16
摘要： The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.
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公开(公告)号：US20070099283A1
公开(公告)日：2007-05-03
申请号：US10467532
申请日：2002-02-08
申请人： Rainer Mueller , Johann-Peter Thalhofer , Bernhard Rexer , Rainer Schmuck , Frank Geipel , Stephan Glaser , Helmut Schoen , Thomas Meier , Rainer Rudolph , Hauke Lilie , Bjoern Schott
发明人： Rainer Mueller , Johann-Peter Thalhofer , Bernhard Rexer , Rainer Schmuck , Frank Geipel , Stephan Glaser , Helmut Schoen , Thomas Meier , Rainer Rudolph , Hauke Lilie , Bjoern Schott
IPC分类号： C12N9/58
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention concerns recombinant proteinase K. Furthermore a method for producing recombinant proteinase K is disclosed, which is characterized in that a) a host cell is transformed with a recombinant nucleic acid which codes for the zymogenic precursor of proteinase K, b) the host cell is cultured in such a manner that the zymogenic precursor of proteinase K is formed in the form of inclusion bodies in the host cell, c) the inclusion bodies are isolated and natured under conditions which result in the formation of the protease part of the zymogenic precursor in its natural conformation, d) the natured proteinase K is activated and purified.
摘要翻译： 本发明涉及重组蛋白酶K.此外，公开了一种生产重组蛋白酶K的方法，其特征在于：a）用编码蛋白酶K的酶原前体的重组核酸转化宿主细胞，b）宿主细胞 以这样的方式培养，使得蛋白酶K的酶原前体以宿主细胞中的包涵体的形式形成，c）包涵体在导致酶原前体的蛋白酶部分的形成的条件下被分离和性质 在其天然构象中，d）性质蛋白酶K被活化和纯化。
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公开(公告)号：US20050142633A1
公开(公告)日：2005-06-30
申请号：US11003149
申请日：2004-12-03
申请人： Stephan Glaser , Frank Geipel , Thomas Kirschbaum , Bernhard Rexer , Johann-Peter Thalhofer , Rainer Mueller , Claudia Giessel , Hellmut Eckstein , Elvira Wolf
发明人： Stephan Glaser , Frank Geipel , Thomas Kirschbaum , Bernhard Rexer , Johann-Peter Thalhofer , Rainer Mueller , Claudia Giessel , Hellmut Eckstein , Elvira Wolf
CPC分类号： C12N9/48 , C07K2319/02 , C07K2319/21
摘要： The invention provides a method to produce a protein with carboxypeptidase B activity from a pro-carboxypeptidase B zymogen, derived from a non-animal host organism. Carboxypeptidase B is activated from the zymogen using non-denaturing conditions. Particularly, the activation is performed under conditions that avoid unwanted non-covalent binding of the propeptide to the activated carboxypeptidase B enzyme.
摘要翻译： 本发明提供从源自非动物宿主生物体的原羧肽酶B酶原产生具有羧肽酶B活性的蛋白质的方法。 羧肽酶B使用非变性条件从酶原激活。 特别地，激活是在避免前肽与活化的羧肽酶B酶的不期望的非共价结合的条件下进行的。
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